牦牛源牛冠状病毒部分基因的扩增、序列分析及病毒分离鉴定

doi:10.11843/j.issn.0366-6964.2019.02.012

Abstract

Abstract:

The aim of this study was to investigate molecular prevalence of bovine coronavirus (BCoV) in yak in Qinghai-Tibetan Plateau and to isolate BCoV from clinical samples. RT-PCR assay was used to detect BCoV from 336 diarrhea feces samples of yak from Tibet, Qinghai, Sichuan and Yunnan, and the S, HE, and N gene fragments were further amplified, moreover, positive samples were used to isolate BCoV strain. Results were as follows:Approximate 69.05% (232/336) diarrheic samples were detected BCoV positive by RT-PCR. Sequence and phylogenetic analysis showed that BCoV S1 subunit sequence, HE gene fragment and N gene fragment from 32 clinical samples in this study had unique evolutionary features. Notably, a BCoV strain of yak was successfully isolated firstly, the TCID₅₀ of this strain was 10^-7.17·0.1 mL^-1,and a recombinant event was predicted in S gene of this strain. These results indicate that BCoV widely circulates in yak in the Qinghai-Tibetan Plateau of China and exhibit unique evolution.

CLC Number:

S852.659.6

HE Qifu, TANG Cheng, GUO Zijing, TAN Shuo, ZHANG Bin, YUE Hua. Amplification, Sequence Analysis of Bovine Coronavirus Genes and Isolation of the Viruses from Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 343-353.

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Amplification, Sequence Analysis of Bovine Coronavirus Genes and Isolation of the Viruses from Yak

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