硫酸小檗碱对猪轮状病毒感染的IPEC-J2中TGF-β1、EGFR、EGF、IGF1 mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2018.10.025

Abstract

Abstract:

This study was conducted to investigate the effects of berberine sulfate (BS) on TGF-β₁, EGFR, EGF and IGF₁ expression in porcine intestinal epithelial cell (IPEC-J2) that were infected by rotavirus. We established a model in which IPEC-J2 were infected with rotavirus. We established target and reference gene recombinant plasmid and standard curve. TGF-β₁, EGFR, EGF and IGF₁ mRNA levels were quantified in BS and rotavirus treated IPEC-J2 by real-time polymerase chain reaction. Results were as follows:after the infection of control IPEC-J2 with rotavirus, the cells clustered into poorly-organized groups, shed or died. By contrast, the BS-treated IPEC-J2 that were infected with rotavirus formed highly-organized groups. The difference of TGF-β₁, EGFR, EGF and IGF₁ gene mRNA expression in each experimental group was compared with that of the control group, showing that BS can promote the expression of TGF-β₁ and EGFR. However, BS inhibited the expression of EGF and IGF₁ mRNA. Compared with EGF and IGF₁ gene expression decreased significantly, the expression of TGF-β₁ and EGFR gene were increased significantly, suggesting that the effect of repair factors may not be the same in the process of repairing cells injury. Our results show that BS could ameliorate IPEC-J2 injury by regulating repair factors expression to promote the proliferation and transformation of cells for repairing the intestinal mucosa.

CLC Number:

S853.7

XU Huan-ye, LI Mei-ling, CHEN Li-gong, WANG Li-ye, MENG Li-jia, CUI Huan, ZHANG Cheng, BAI Xiao, WANG Ying-chun, DONG Shi-shan. Berberine Sulfate Affects TGF-β₁, EGFR, EGF and IGF₁ mRNA Expression in Rotavirus-infected IPEC-J2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2283-2291.

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Berberine Sulfate Affects TGF-β₁, EGFR, EGF and IGF₁ mRNA Expression in Rotavirus-infected IPEC-J2

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Berberine Sulfate Affects TGF-β1, EGFR, EGF and IGF1 mRNA Expression in Rotavirus-infected IPEC-J2

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Berberine Sulfate Affects TGF-β₁, EGFR, EGF and IGF₁ mRNA Expression in Rotavirus-infected IPEC-J2