不同来源的3株牛病毒性腹泻病毒对小鼠的致病性分析

doi:10.11843/j.issn.0366-6964.2018.10.019

Abstract

Abstract:

This study aims at analyzing the pathogenicity of bovine viral diarrhea virus (BVDV) in mice, BALB/c mice were selected as experimental animals in this study, the challenge group were inoculated with yak-derived BVDV1-DJ2, BVDV1-Z6 and Oregon C₂₄V strains respectively by intraperitoneal injection, and the control group were inoculated with DMEM medium. The blood and tissues of the mice were collected on the 5th, 7th, and 10th day after inoculation, and the pathogenicity of the virus-infected mice were studied by blood routine, histopathology, and fluorescence quantitative PCR. The results showed, after the challenge, the clinical symptoms of the mice in the Z6 group were more severe than those in the DJ2 group. The lymphocyte, leukocyte, and platelet contents of the challenge group decreased significantly. The results of the fluorescence quantitative PCR showed that the BVDV was detected in the lung and liver. The rate of Z6 group was significantly higher than that of DJ2 group. HE staining showed that there were inflammatory cell infiltration, necrosis and shedding of intestinal villus epithelial cells in the liver of the challenge group, and it also showed that there were increased spleen endocytosis and reactive hyperplasia of spleen, alveolar atrophy and hemorrhage and other pathological changes. Compared with the Oregon C₂₄V group, the lesion of Z6 group were the most serious and the lesion of DJ2 group was lighter. Using BALB/c mice as experimental animals and the way of intraperitoneal injection, this study can provide data support and guiding significance for the pathogenicity of BVDV-infected mice and the construction of models.

CLC Number:

S852.659.6

RUAN Wen-qiang, CHEN Xin-nuo, REN Yu-peng, QIN Si-nan, TANG Cheng, ZHANG Bin. Pathogenicity Analysis of Three Bovine Viral Diarrhea Viruses from Different Sources in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2232-2239.

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References

[1] HOUE H. Epidemiological features and economical importance of bovine virus diarrhoea virus (BVDV) infections[J]. Vet Microbiol, 1999, 64(2-3):89-107.
[2] BACHOFEN C, VOGT H R, STALDER H, et al. Persistent infections after natural transmission of bovine viral diarrhoea virus from cattle to goats and among goats[J]. Vet Res, 2013, 44(1):32.
[3] KIRCHGESSNER M S, DUBOVI E J, WHIPPS C M. Spatial point pattern analyses of Bovine viral diarrhea virus infection in domestic livestock herds and concomitant seroprevalence in wild white-tailed deer (Odocoileus virginianus) in New York State, USA[J]. J Vet Diagn Invest, 2013, 25(2):226-233.
[4] PASSLER T, DITCHKOFF S S, GIVENS M D, et al. Transmission of bovine viral diarrhea virus among white-tailed deer (Odocoileus virginianus)[J]. Vet Res, 2010, 41(2):20.
[5] NELSON D D, DARK M J, BRADWAY D S, et al. Evidence for persistent Bovine viral diarrhea virus infection in a captive mountain goat (Oreamnos americanus)[J]. J Vet Diagn Invest, 2008, 20(6):752-759.
[6] CARMAN S, CARR N, DELAY J, et al. Bovine viral diarrhea virus in alpaca:abortion and persistent infection[J]. J Vet Diagn Invest, 2005, 17(6):589-593.
[7] SEONG G, OEM J K, CHOI K S. The first finding of the ophthalmosteresis in a Korean native calf with bovine viral diarrhea-case report[J]. Vet Arh, 2015, 85(1):111-116.
[8] DUBOVI E J. Impact of bovine viral diarrhea virus on reproductive performance in cattle[J]. Vet Clin North Am Food Anim Pract, 1994, 10(3):503-514.
[9] BROWNLIE J, CLARKE M C, HOWARD C J. Experimental production of fatal mucosal disease in cattle[J]. Vet Rec, 1984, 114(22):535-536.
[10] CORAPI W V, ELLIOTT R D, FRENCH T W, et al. Thrombocytopenia and hemorrhages in veal calves infected with bovine viral diarrhea virus[J]. J Am Vet Med Assoc, 1990, 196(4):590-596.
[11] SPAGNUOLO M, KENNEDY S, FOSTER J C, et al. Bovine viral diarrhoea virus infection in bone marrow of experimentally infected calves[J]. J Comp Pathol, 1997, 116(1):97-100.
[12] WOOD R D, GOENS S D, CARMAN P S, et al. Effect on hematopoietic tissue of experimental infection of calves with noncytopathic type 2 bovine viral diarrhea virus[J]. Can J Vet Res, 2004, 68(1):42-48.
[13] WALZ P H, BELL T G, STEFICEK B A, et al. Experimental model of type Ⅱ bovine viral diarrhea virus-induced thrombocytopenia in neonatal calves[J]. J Vet Diagn Invest, 1999, 11(6):505-514.
[14] CORAPI W V, FRENCH T W, DUBOVI E J. Severe thrombocytopenia in young calves experimentally infected with noncytopathic bovine viral diarrhea virus[J]. J Virol, 1989, 63(9):3934-3943.
[15] MARSHALL D J, MOXLEY R A, KELLING C L. Distribution of virus and viral antigen in specific pathogen-free calves following inoculation with noncytopathic bovine viral diarrhea virus[J]. Vet Pathol, 1996, 33(3):311-318.
[16] 王雪枝. 牛病毒性腹泻病毒E0蛋白单抗与多抗制备及实验感染小鼠的研究[D]. 北京:中国农业科学院, 2014.
WANG X Z.Preparations of monoclonal antibody and polyclonal antibody against E0protein of bovine viral diarrhea virus and experimental infection of mice with the virus[D]. Beijing:Chinese Academy of Agricultural Sciences, 2014. (in Chinese)
[17] 李彩虹,何文,王怀禹. 牛病毒性腹泻病毒四川株SC的分离鉴定及其生物学特性分析[J]. 畜牧与兽医, 2017, 49(1):75-79.
LI C H, HE W, WANG H Y.Isolation and identification of bovine viral diarrhea virus Sichuan strain SC and analysis of its biological characteristics[J]. Animal Husbandry & Veterinary Medicine, 2017, 49(1):75-79. (in Chinese)
[18] 王炜. 牛主要呼吸道病毒病血清学调查、牛病毒性腹泻病毒分离株鉴定及疫苗研究[D]. 北京:中国农业科学院, 2014.
WANG W.Serosurvey of major bovine resporitary viruses and identification of BVDV isolates and vaccine development[D]. Beijing:Chinese Academy of Agricultural Sciences, 2014. (in Chinese)
[19] 周霞. BVDV在人工感染绵羊体内抗原定位的试验研究[D]. 乌鲁木齐:新疆农业大学, 2001.
ZHOU X. Study on antigen location of BVDV in artifical infected sheep[D]. Urumchi:Xinjiang Agricultural University, 2001. (in Chinese)
[20] BACHOFEN C, GRANT D M, WILLOUGHBY K, et al. Experimental infection of rabbits with bovine viral diarrhoea virus by a natural route of exposure[J]. Vet Res, 2014, 45(1):34.
[21] SEONG G, LEE J S, LEE K H, et al. Experimental infection with cytopathic bovine viral diarrhea virus in mice induces megakaryopoiesis in the spleen and bone marrow[J]. Arch Virol, 2016, 161(2):417-424.
[22] RAYA A I, GOMEZ-VILLAMANDOS J C, BAUTISTA M J. Role of thymic epithelial cells in lymphoid depletion after experimental infection with the noncytopathogenic BVDV1 strain 7443[J]. Vet Pathol, 2015, 52(2):291-294.
[23] SEONG G, LEE J S, LEE K H, et al. Noncytopathic bovine viral diarrhea virus 2 impairs virus control in a mouse model[J]. Arch Virol, 2016, 161(2):395-403.
[24] SEONG G, OEM J K, LEE K H, et al. Experimental infection of mice with bovine viral diarrhea virus[J]. Arch Virol, 2015, 160(6):1565-1571.

Pathogenicity Analysis of Three Bovine Viral Diarrhea Viruses from Different Sources in Mice

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