猴头菇多糖对氧化应激状态下仔猪回肠形态结构及通透性的影响

doi:10.11843/j.issn.0366-6964.2018.01.023

Abstract

Abstract:

This study aims to investigate the effects of feeding Hericium erinaceus polysaccharide (HEP) to weaned piglets on ileal morphology and permeability under oxidative stress. Forty-five healthy long-white weaned piglets with similar weight were randomly divided into five groups (every group had 9 replications). Control group and model group were fed with basic diet only, meanwhile the other three test groups were fed with basic diet added with 0.2%, 0.4% or 0.6% HEP. On the 21st day, piglets in model group and three test groups were injected with 100 μg·kg^-1·BW lipopolysaccharide, meanwhile, piglets in control group were injected with equal dose sterilized normal saline. Blood samples were collected to detect antioxidant functions and intestinal mucosal integrity. The piglets were slaughtered and the ileums were collected to observe the morphological changes by HE staining; the expression of tight junction proteins of ileum were detected by qPCR methods and Western blot methods. The results showed that, compared with the model group, HEP could significantly improve the decrease of serum antioxidant indexes caused by LPS (P<0.05), significantly increase glutamine (GLN) content (P<0.05) and reduce diamine oxidase (DAO) activity (P<0.05); and significantly recovery the decrease of tight junction protein expression in ileum epithelial cells induced by LPS stress (P<0.05). Supplementation of HEP can effectively improve the permeability of ileal morphology under oxidative stress.

CLC Number:

S853.7

ZHANG Jian-long, QIU Fu-an, DONG Xing, QIN Tao, MA Yu-fang, HUANG Yi-fan, LI Jian. Effects of Hericium Erinaceus Polysaccharide on Ileal Morphology and Permeability of Piglets under Oxidative Stress[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 203-210.

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Effects of Hericium Erinaceus Polysaccharide on Ileal Morphology and Permeability of Piglets under Oxidative Stress

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