不同猪支原体肺炎活疫苗菌株P46和P97基因序列差异性分析

doi:10.11843/j.issn.0366-6964.2017.12.016

Abstract

Abstract:

This experiment was conducted to study the conservation and variation of P46 gene and P97R1R2 gene of three Mycoplasmal pneumonia of swine vaccine strains, e.g.168, RM48 and Z, from domestic production. The DNA of the four vaccine manufacturer products were extracted. The target gene fragments amplified by PCR were sequenced. And the nucleotides sequence and the deduced amino acids sequences were analyzed by DNAStar software, which compared with four international standard strains.The attenuated vaccine strain 168 showed 100% homology of P46 nucleotides sequence with vaccine strain Z and RM48, while 99.1% of that with strain J and 232, 98.9% of that with strain 7422 and 7448. As for P97R1R2 gene, strain Z and RM48 showed completely same identity, and 97.5% of identity with strain 168. The homology among vaccine strains, strain J, 232, 7448 and strain 7422 was lower, only from 90.1% to 93.6%. The major differences of the vaccine strains occurred in the repeat numbers of P97R1 amino acid sequence, which is benefit for differentiation of the attenuated vaccine strains.

CLC Number:

S852.62

WU Yu-zi, XING Xian-ping, WEI Yan-na, XIONG Qi-yan, FENG Zhi-xin, SHAO Guo-qing. Comparison of Nucleotide and Deduced Amino Acid Sequences of P46 and P97 for the Different Live Vaccine Strains of Mycoplasmal Pneumonia of Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2365-2373.

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Comparison of Nucleotide and Deduced Amino Acid Sequences of P46 and P97 for the Different Live Vaccine Strains of Mycoplasmal Pneumonia of Swine

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