经密码子优化的猪Ficolin α在昆虫细胞的表达及其体外抗病毒作用初步分析

doi:10.11843/j.issn.0366-6964.2017.12.015

Abstract

Abstract:

To get native porcine Ficolin α, Bac-to-Bac baculovirus expression system was used. Firstly, rBacmid-Ficolin α was constructed, and then transfected into sf9 insect cells to obtain recombinant baculovirus rBV-Ficolin α. The optimized expression conditions were detected by Western blot and analyzed by gray level using Image J software. Followed by purification, antiviral activity of target protein was assessed in vitro. Western blot results showed that the expression level of Ficolin α in transfected sf 9 insect cells was higher at the infection (MOI) of 7.5 when they were harvested at 96 h, and porcine Ficolin α had the antivirus activity against porcine reproductive and respiratory syndrome virus (PRRSV). In conclusion, porcine Ficolin α was successfully expressed in sf9 insect cells and showed strongly anti-PRRSV activity. This study provided a material basis for the study of antiviral activity and its mechanism of porcine Ficolin α.

CLC Number:

S852.42

LI Lan, QIAO Xu-wen, ZHANG Yuan-peng, CHEN Jin, ZHENG Qi-sheng, HOU Ji-bo. Expression of Codon-optimized Porcine Ficolin α in Insect Cell and Its Antiviral Activity in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2358-2364.

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Expression of Codon-optimized Porcine Ficolin α in Insect Cell and Its Antiviral Activity in vitro

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