O型口蹄疫病毒不同宿主适应毒株P1和3A基因的差异分析

doi:10.11843/j.issn.0366-6964.2017.05.012

Abstract

Abstract:

Difference of P1 and 3A gene sequences of different hosts-adapted strains type O Foot-and mouth disease virus (FMDV) were identified,and the trend of genetic variation were studied to find the genetic mutation of P1 and 3A genes of different hosts-adapted FMDV. FMDV O/XJ/10-11 strain was inoculated into bovine, neonatal rat, swine and BHK-21 cell line to obtain corresponding adapted virus strains. The RNA of FMDV in each adapted virus was taken as template for reverse transcription and amplification of P1(1A, 1B, 1C, 1D)and 3A genes. Fragment was purified and recovered by agarose gel electrophoresis,cloned into the vector pMD19-T. Positive colonies were screened,sequenced, and the gene sequences were analyzed. The results were as follows：no deletions were found in nucleotide and amino acid sequences of different hosts-adapted virus strains,the main antigenic sites were conserved in different hosts；The P1 gene of the bovine,swine,neonatal rat,and BHK-21 cells adapted virus strains had some variations,the variation extent decreased in sequence of VP1,VP2 > VP3> VP4, and VP4 was the most conserved gene region；3A gene was more stable and showed less variation；The most varied host-adapted FMDV was from swine, followed by BHK-21 cells, bovine and neonatal rat. The results showed that there is greater variability in the gene of VP1, however, the main antigenic sites of VP1 were conserved；VP2 mutations were located in its antigenic epitope; as the storage of the original virus of FMDV, neonatal rat-adapted virus is more favorable.

CLC Number:

S852.659.6

YAO Huai-bing, ZHAO Yi, WANG Jin-quan, LIU Meng-li, LIU Hong, REN Fang, HUANG Jiong. Studies on Variations of P1 and 3A Genes of Different Hosts-adapted Strains Type O Foot-and-mouth Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 881-888.

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Studies on Variations of P1 and 3A Genes of Different Hosts-adapted Strains Type O Foot-and-mouth Disease Virus

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