腺病毒介导的MAT2B过表达促进猪肌内脂肪细胞分化

doi:10.11843/j.issn.0366-6964.2017.05.003

Abstract

Abstract:

The aim of this study was to explore the effect of MAT2B on porcine intramuscular preadipocyte differentiation by constructing overexpression vector in vitro mediated by adenovirus. A pair of exclusive primers was designed according to the GenBank sequence information of MAT2B gene, and MAT2B was amplified by PCR. The obtained PCR products were inserted into a shuttle vector pAdTrack-CMV, then the gene was identified by sequencing. The results showed that shuttle vector (pAdTrack-CMV) and backbone vector (pAdEasy-1) were in homologous recombination, and the adenovirus recombinant vector (pAd-MAT2B) was constructed successfully. Then the recombinant adenovirus DNA was digested by the Pac I, the pAd-MAT2B adenovirus was produced, and we used it to infect the porcine preadipocytes. The expression level of mRNA and protein of MAT2B significantly increased. Oil Red O staining assay showed that overexpression of MAT2B promoted lipid accumulation in intramuscular preadipocyte. Moreover, the expression of adipogenic gene PPARγ and aP2 mRNA were significantly promoted when infected by Ad-MAT2B. The results suggest that MAT2B is overexpressed in expression vector in vitro mediated by adenovirus, and MAT2B play a positive role during porcine intramuscular preadipocyte differentiation.

CLC Number:

S828
S813.1

ZHAO Cun-zhen, LI Huang-kun, MA Yun, YANG Gong-she. MAT2B Overexprssion Mediated by Adenovirus Promoting Porcine Intramuscular Preadipocyte Differentiation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 802-809.

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MAT2B Overexprssion Mediated by Adenovirus Promoting Porcine Intramuscular Preadipocyte Differentiation

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