SPF雏鸡感染REV后中枢免疫器官细胞凋亡及相关因子的变化

doi:10.11843/j.issn.0366-6964.2017.010.022

Abstract

Abstract:

The research was conducted to study the effects of reticuloendotheliosis virus (REV) on cell apoptosis, enzyme activities and expression of relevant factors in immune organs of chicks. Seventy one-day old SPF chicks were randomly selected into REV infection groups (I) and control groups (C). The methods of enzyme activities detection kit, RT-PCR, ELISA and TUNEL were applied to detect activities of Caspase-3 and Caspase-9, expression of Bcl-2 and cell apoptosis in central immune organs. The results showed that the detected indexes in thymus of SPF chicks infected with REV were significantly higher than control groups at 7 to 28 days after infection. The indexes in bursa of Fabricius of SPF chicks infected with REV were significantly increased than control groups at 7 to 35 days after infection. These data indicate that cell apoptosis caused by enhancement of Caspase-3 and Caspase-9 activities, up-regulated expression of the Bcl-2 mRNA and protein in central immune organs of SPF chicks infected with REV, are closely related to immune function suppression.

CLC Number:

S858.315.3

FU Li-sheng, HOU Ning, WANG Xiao-yan, GAO Xue-li, LIU Chao-nan, Lü Xiao-ping, ZHENG Shi-min. Changes of Cell Apoptosis and Relevant Factor in Central Immune Organs of SPF Chicks Infected with Reticuloendotheliosis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1983-1989.

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Changes of Cell Apoptosis and Relevant Factor in Central Immune Organs of SPF Chicks Infected with Reticuloendotheliosis Virus

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