phoP/Q双组分系统对禽致病性大肠杆菌的毒力调控作用

doi:10.11843/j.issn.0366-6964.2016.01.021

Abstract

Abstract:

As a kind of external environment receptor，phoP/phoQ is involved in regulating bacterial adaptability to external environment.The aim of this study was to investigate the effect of phoP/Q two-component system to biological characteristics of avian pathogenic Escherichia coli (APEC) such as growth，engraftment，and virulence.The phoP-phoQ deletion mutant strain was constructed by homologous recombination assay，and the complement strain was constructed through transformation deletion strain with the plasmid complement.The growth curve determination and motility assay，the adhesion and invasion to chicken embryo fibroblast (CEF)，detection of virulence gene transcription and median lethal dose (LD₅₀) experimental were used to compare biological characteristics of wild strain，deletion strain and complement strain.Compared with the wild strain，the growth characteristics of the mutant strain did not change；the motility of the mutant strain felled by 63% comparing with wild strain；its ability of adhesion and invasion to DEF cells respectively decreased to 69.83% and 62.17%；and LD₅₀ was significantly reduced by 13.3 times.The transcription of polA，tsh respectively increased by 41% and 54%，while the sodA and iss decreased by 64% and 98%.The phoP/Q two-component system is involved in the regulation of the APEC pathogenic，and its deletion can reduce the pathogenicity of APEC.

CLC Number:

S852.612

LI Chun-xiao，ZHANG Yu-xi，QI Ke-zong，HAN Xian-gan，TU Jian，XUE Ting，ZHOU Xiu-hong. The Role of phoP/Q Two-component System in Virulence of Avian Pathogenic Escherichia coli[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 157-164.

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The Role of phoP/Q Two-component System in Virulence of Avian Pathogenic Escherichia coli

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