羊伪结核棒状杆菌的分离鉴定及部分生物学特性分析

doi:10.11843/j.issn.0366-6964.2024.02.025

摘要/Abstract

摘要： 为明确重庆和贵州部分羊场发生体表淋巴结脓肿病料中伪结核棒状杆菌(Corynebacterium pseudotuberculosis,Cp) 感染情况及其生物学特征,对山羊体表脓肿样本进行Cp分离鉴定、致病性试验、药敏试验、毒力/耐药基因检测及系统进化分析。结果:从40份样本中分离到23株Cp,分离率达57.5%。毒力基因检测显示,所有Cp分离菌均携带pld、FagA、FagB、OppB、OppD、OppF、SodC、SpaC、pknG、NanH、sigE和cp40基因。药敏试验显示,所分离Cp对头孢拉定、丁胺卡那和多西环素敏感率最高,达95.65%(22/23),其次为青霉素、氨苄西林、四环素、万古霉素和环丙沙星,敏感率为91.30%(21/23),但对呋喃唑酮100%耐药,对新霉素和卡那霉素耐药率为82.61%(19/23)。Cp分离菌中氨基糖苷类耐药基因aph(3')-IIa和广谱A类β内酰胺酶耐药基因bla_TEM-116的检出率分别为0和4.35%。对所有Cp的fusA进行测序并获得GenBank登录号ON969140-ON969162,系统进化分析显示所分离的Cp均聚类到羊生物型(biotype ovis)。本研究证实Cp是引起重庆和贵州部分羊场发生体表淋巴结脓肿的主要病原,并明确其部分生物学特性,为防控该病原提供了参考资料。

关键词: 伪结核棒状杆菌, 山羊, 毒力基因, 药物敏感性, 系统进化分析

Abstract: To clarify the infection and biological characteristics of Corynebacterium pseudotuberculosis (Cp) in surface lymph node abscesses of goats in Chongqing and Guizhou, Cp isolation and identification, pathogenicity tests, antimicrobial susceptibility tests, virulence or antibiotics resistance gene detection and phylogenetic analysis were carried out in this study. The results showed that 23 strains of Cp were isolated from 40 samples, with a separation rate of 57.5%. All the Cp isolates harbored pld, FagA, FagB, OppB, OppD, OppF, SodC, SpaC, pknG, NanH, sigE, and cp40 genes. The results of antimicrobial susceptibility tests showed that 95.65% (22/23) of Cp was sensitive to cefradine, amikacin and doxycycline, followed by penicillin, ampicillin, tetracycline, vancomycin and ciprofloxacin, with a sensitivity rate of 91.30% (21/23), while 100% of Cp isolates were resistant to furazolidone and 82.61% (19/23) resistant to neomycin and kanamycin. The detection rate of aminoglycoside O-phosphotransferase gene aph(3')-IIa and broad-spectrum class A beta-lactamase gene bla_TEM-116 were 0 and 4.35% respectively. The fusA gene of Cp was sequenced and submitted to GenBank with login numbers ON969140-ON969162, and phylogenetic analysis of fusA showed that all the Cp isolates were clustered into Cp biotype ovis. This study verified that Cp was the major pathogen responsible for surface lymph node abscesses of goats in Chongqing and Guizhou, and clarified its biological characteristics which provided information for this pathogen prevention.

Key words: Corynebacterium pseudotuberculosis, goat, virulence genes, antimicrobial susceptibility, phylogenetic analysis

中图分类号:

S852.61

宋艳, 袁永丰, 钱虹宇, 李鑫灿, 罗洪艳, 王芝英, 周作勇. 羊伪结核棒状杆菌的分离鉴定及部分生物学特性分析[J]. 畜牧兽医学报, 2024, 55(2): 680-687.

SONG Yan, YUAN Yongfeng, QIAN Hongyu, LI Xincan, LUO Hongyan, WANG Zhiying, ZHOU Zuoyong. Identification and Partial Biological Characteristics Analysis of Corynebacterium pseudotuberculosis Isolated from Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 680-687.

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