河北省廊坊市牛主要病毒性腹泻病原感染状况检测及牛冠状病毒演化分析

doi:10.11843/j.issn.0366-6964.2024.02.022

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (2): 649-659.doi: 10.11843/j.issn.0366-6964.2024.02.022

河北省廊坊市牛主要病毒性腹泻病原感染状况检测及牛冠状病毒演化分析

李思远^1,2, 付新成⁵, 袁雪松^2,3, 毛立^2,3, 蔡旭航^1,2, 孙心如², 黄金^2,3, 谢玲玲⁶, 王府⁶, 周华⁷, 张琪^1*, 李基棕^2,3,4*, 李彬^2,3,4*

1. 西北农林科技大学动物医学院, 杨凌 712100;
2. 江苏省农业科学院兽医研究所, 农业农村部兽用生物制品工程技术重点实验室, 南京 210014;
3. 南京农业大学动物医学院, 南京 210095;
4. 江苏大学生命科学学院食品与生物工程学院, 镇江 212013;
5. 河北省廊坊市农业农村局, 廊坊 065000;
6. 贵州省种畜禽种质测定中心, 贵阳 550018;
7. 黔西市动物疫病预防控制中心, 黔西 551500

收稿日期:2023-04-18 出版日期:2024-02-23 发布日期:2024-02-27
通讯作者: 张琪,主要从事牛病学研究,E-mail:273010466@qq.com,李基棕,主要从事动物传染病防治和诊断技术研究,E-mail:lijizong22@sina.com;李彬,主要从事动物病毒学研究,E-mail:libinana@126.com
作者简介:李思远(1999-)男,福建福州人,硕士生,主要从事牛腹泻病原研究,E-mail:lisy990102@163.com;付新成(1978-),男,河北唐山人,本科,高级兽医师,主要从事动物疫病防控研究,E-mail:670322997@qq.com
基金资助:
十四五国家重点研发计划(2021YFD1801101);陕西省重点研发计划一般项目(2022NY-098);贵州省种业发展项目(肉牛遗传改良与高效养殖技术研究);江苏省农业科技自主创新资金项目(CX(21)3137)

Detection of Bovine Viral Diarrhea Pathogens and Evolution Analysis of Bovine Coronavirus in Langfang, Hebei

LI Siyuan^1,2, FU Xincheng⁵, YUAN Xuesong^2,3, MAO Li^2,3, CAI Xuhang^1,2, SUN Xinru², HUANG Jin^2,3, XIE Lingling⁶, WANG Fu⁶, ZHOU Hua⁷, ZHANG Qi^1*, LI Jizong^2,3,4*, LI Bin^2,3,4*

1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;
2. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China;
3. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
4. School of Food and Biological Engineering, School of Life Sciences, Jiangsu University, Zhenjiang 212013, China;
5. Langfang Municipal Bureau of Agriculture and Rural Affairs, Langfang 065000, China;
6. Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, China;
7. Qianxi Animal Disease Control Center, Qianxi 551500, China

Received:2023-04-18 Online:2024-02-23 Published:2024-02-27

摘要/Abstract

摘要： 牛病毒性腹泻病原严重危害畜牧业生产,BVDV、BoAstV、BCoV、MRV、BKoV、BoNeV、BNoV、BRV、BToV被认为是引起牛群腹泻的病原,不仅如此,BVDV、BCoV和BToV除了能引起消化道症状外还能引起呼吸道症状,BVDV不仅能引起消化道和呼吸道症状,还会导致母牛生殖功能异常,这些疾病对畜牧业造成巨大的经济损失。为了解牛主要病毒性腹泻病原流行情况,本次试验通过PCR检测方法对我国河北地区323份腹泻牛粪样品进行常见9种病毒性牛腹泻病原进行检测。结果显示,BVDV阳性检出率1.85% (6/323)、 BCoV阳性检出率14.24% (46/323)、BRV阳性检出率57.89% (187/323)、BoAstV阳性检出率0.31% (1/323)、BoNeV阳性检出率10.84% (35/323)、BNoV阳性检出率4.33%(14/323)、MRV阳性检出率2.48% (8/323)、BToV阳性检出率4.64% (15/323)、BKoV阳性检出率11.76% (38/323)。混合感染共有76份,占比23.53%(76/323)。HBLF2302株的全基因序列、S、HE、N、M和E基因进行同源性和遗传进化分析发现,HBLF2302与BCoV/NMG1/2022基因同源性最高,为GIIb亚型。基于氨基酸序列比对和HBLF2302的S蛋白三级结构预测发现,在S蛋白中157号位突变为157T,854号位点突变为854I。318V为独特突变,改变了与侧链残基的作用力,与631C形成氢键连接。并发现中国株区别于其它地区的GⅡb亚型,位于ORF1a区域有三个独特位点。本研究丰富了牛腹泻病原的流行病学数据,为牛腹泻病的防控奠定基础。

关键词: 病毒性腹泻, 牛冠状病毒, 流行病学调查, 腹泻病原

Abstract: Cattle viral diarrhea pathogens have adverse effects on animal husbandry. BVDV, BoAstV, BCoV, MRV, BKoV, BoNeV, BNoV, BRV, BToV were well known as the cause of diarrhea. On the other hand, BVDV, BCoV and BToV also caused respiratory system symptoms. Among them, BVDV not only causes alimentary tract symptoms and respiratory symptoms, but also leads to abnormal reproductive function of cows causing great economic losses. In order to understand the prevalence of the main viral diarrhea pathogens in cattle, 323 fecal samples from diarrheal cow in Hebei province of China were detected by PCR to detect nine common viral diarrhea pathogens. Prevalence of 1.85% (6/323), 14.24% (46/323), 57.89% (187/323), 0.31 (1/323), 10.84% (35/323), 4.33% (14/323), 2.48% (8/323), 4.64% (15/323), 11.76% (38/323), was determined for BVDV, BCoV, BRV, BoAstV, BoNeV, BNoV, MRV, BToV, BKoV, respectively. There were 76 co-infection samples accounting for 23.53% (76/323). Homology and genetic evolution analysis of the complete genomic sequence, S, HE, N, M and E genes of HBLF2302 strain showed that HBLF2302 gene had high homology with BCoV/NMG1/2022 and was GIIb subtype. Based on the amino acid sequence alignment and the tertiary structure prediction of HBLF2302 S protein, it was found that the 157 site was mutated to 157T and the 854 site was mutated to 854I in the S protein. 318V is a unique mutation, which changes the force with the side chain residue and forms a hydrogen bond connection with 631C. It was found that the Chinese strain was different from the GⅡb subtype in other regions, and there were three unique sites in the ORF1a region. This study enriched the epidemiological data about bovine diarrhea pathogens for laying the foundation of bovine pathogens prevention and control.

Key words: viral diarrhea, bovine coronavirus, epidemiology investigation, diarrhea pathogens

中图分类号:

S852.653

李思远, 付新成, 袁雪松, 毛立, 蔡旭航, 孙心如, 黄金, 谢玲玲, 王府, 周华, 张琪, 李基棕, 李彬. 河北省廊坊市牛主要病毒性腹泻病原感染状况检测及牛冠状病毒演化分析[J]. 畜牧兽医学报, 2024, 55(2): 649-659.

LI Siyuan, FU Xincheng, YUAN Xuesong, MAO Li, CAI Xuhang, SUN Xinru, HUANG Jin, XIE Lingling, WANG Fu, ZHOU Hua, ZHANG Qi, LI Jizong, LI Bin. Detection of Bovine Viral Diarrhea Pathogens and Evolution Analysis of Bovine Coronavirus in Langfang, Hebei[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 649-659.

参考文献

[1] CLARK M A. Bovine coronavirus[J]. Br Vet J, 1993, 149(1):51-70.
[2] TSUNEMITSU H, SAIF L J. Antigenic and biological comparisons of bovine coronaviruses derived from neonatal calf diarrhea and winter dysentery of adult cattle[J]. Arch Virol, 1995, 140(7):1303-1311.
[3] RAHE M C, MAGSTADT D R, GROELTZ-THRUSH J, et al. Bovine coronavirus in the lower respiratory tract of cattle with respiratory disease[J]. J Vet Diagn Invest, 2022, 34(3):482-488.
[4] SOULES K R, RAHE M C, PURTLE L, et al. Bovine coronavirus infects the respiratory tract of cattle challenged intranasally[J]. Front Vet Sci, 2022, 9:878240.
[5] COLLINS A R. Human coronavirus OC43 interacts with major histocompatibility complex class I molecules at the cell surface to establish infection[J]. Immunol Invest, 1994, 23(4-5):313-321.
[6] PENG G Q, XU L Q, LIN Y L, et al. Crystal structure of bovine coronavirus spike protein lectin domain[J]. J Biol Chem, 2012, 287(50):41931-41938.
[7] COLLINS A R. HLA class I antigen serves as a receptor for human coronavirus OC43[J]. Immunol Invest, 1993, 22(2):95-103.
[8] SZCZEPANSKI A, OWCZAREK K, BZOWSKA M, et al. Canine respiratory coronavirus, bovine coronavirus, and human coronavirus OC43:receptors and attachment factors[J]. Viruses, 2019, 11(4):328.
[9] ERLES K, TOOMEY C, BROOKS H W, et al. Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease[J]. Virology, 2003, 310(2):216-223.
[10] VLASOVA A N, SAIF L J. Bovine coronavirus and the associated diseases[J]. Front Vet Sci, 2021, 8:643220.
[11] ZHANG X M, HERBST W, KOUSOULAS K G, et al. Biological and genetic characterization of a hemagglutinating coronavirus isolated from a diarrhoeic child[J]. J Med Virol, 1994, 44(2):152-161.
[12] AMER H M. Bovine-like coronaviruses in domestic and wild ruminants[J]. Anim Health Res Rev, 2018, 19(2):113-124.
[13] DEREGT D, TESSARO S V, BAXI M K, et al. Isolation of bovine viral diarrhoea viruses from bison[J]. Vet Record, 2005, 157(15):448-450.
[14] BROCK K V. The many faces of bovine viral diarrhea virus[J]. Vet Clin North Am:Food Anim Pract, 2004, 20(1):1-3.
[15] CAFFARENA R D, CASAUX M L, SCHILD C O, et al. Causes of neonatal calf diarrhea and mortality in pasture-based dairy herds in Uruguay:a farm-matched case-control study[J]. Braz J Microbiol, 2021, 52(2):977-988.
[16] ZHU J, QI M P, JIANG C W, et al. Prevalence of bovine astroviruses and their genotypes in sampled Chinese calves with and without diarrhoea[J]. J Gen Virol, 2021, 102(8):001640.
[17] PAPP H, LÁSZLÓ B, JAKAB F, et al. Review of group A rotavirus strains reported in swine and cattle[J]. Vet Microbiol, 2013, 165(3-4):190-199.
[18] BÁNYAI K, ESONA M D, MIJATOVIC S, et al. Zoonotic bovine rotavirus strain in a diarrheic child, Nicaragua[J]. J Clin Virol, 2009, 46(4):391-393.
[19] DRAKER R, ROPER R L, PETRIC M, et al. The complete sequence of the bovine torovirus genome[J]. Virus Res, 2006, 115(1):56-68.
[20] HOET A E, CHO K O, CHANG K O, et al. Enteric and nasal shedding of bovine torovirus (Breda virus) in feedlot cattle[J]. Am J Vet Res, 2002, 63(3):342-348.
[21] ITO T, OKADA N, OKAWA M, et al. Detection and characterization of bovine torovirus from the respiratory tract in Japanese cattle[J]. Vet Microbiol, 2009, 136(3-4):366-371.
[22] SMILEY J R, CHANG K O, HAYES J, et al. Characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus[J]. J Virol, 2002, 76(20):10089-10098.
[23] CHO E H, SOLIMAN M, ALFAJARO M M, et al. Bovine nebovirus interacts with a wide spectrum of histo-blood group antigens[J]. J Virol, 2018, 92(9):e02160-17.
[24] LI H P, TANG C, YUE H. Molecular detection and genomic characteristics of bovine kobuvirus from dairy calves in China[J]. Infect Genet Evol, 2019, 74:103939.
[25] WANG L Y, FREDRICKSON R, DUNCAN M, et al. Bovine kobuvirus in calves with diarrhea, United States[J]. Emerg Infect Dis, 2020, 26(1):176-178.
[26] WU Q, LI J Z, WANG W, et al. Next-generation sequencing reveals four novel viruses associated with calf diarrhea[J]. Viruses, 2021, 13(10):1907.
[27] LI H, ZHANG B, YUE H, et al. First detection and genomic characteristics of bovine torovirus in dairy calves in China[J]. Arch Virol, 2020, 165(7):1577-1583.
[28] GUO Z J, HE Q F, YUE H, et al. First detection of Nebovirus and Norovirus from cattle in China[J]. Arch Virol, 2018, 163(2):475-478.
[29] 吕珽, 陈虹吟, 汤承, 等. 牦牛源正呼肠孤病毒2型的检测和分离鉴定[J]. 畜牧兽医学报, 2021, 52(8):2361-2368.
LV T, CHEN H Y, TANG C, et al. Detection, isolation and identification of mammalian orthoreovirus serotype 2 in Yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8):2361-2368.(in Chinese)
[30] REUTER G, BOLDIZSÁR Á, PANKOVICS P. Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae[J]. Arch Virol, 2009, 154(1):101-108.
[31] 孙飞雁, 叶京飞, 魏宇, 等. 吉林省肉牛冠状病毒感染状况调查及分析[J]. 畜牧兽医学报, 2023, 54(2):673-82.
SUN F Y, YE J F, WEI Y, et al. Epidemiological investigation and analysis of bovine coronavirus in beef cattle in Jilin province[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2):673-682.(in Chinese)
[32] 张松梅, 王鑫文, 代琦, 等. 云南省部分地区牛冠状病毒检测及其N基因序列分析[J]. 中国动物检疫, 2022, 39(11):35-40.
ZHANG S M, WANG X W, DAI Q, et al. Detection of bovine coronavirus and analysis on its N gene sequence in some areas of Yunnan province[J]. China Animal Health Inspection, 2022, 39(11):35-40.(in Chinese)
[33] 张坤, 剡根强, 王静梅, 等. 新疆北疆部分地区致犊牛腹泻冠状病毒的分子流行病学调查[J]. 中国兽医科学, 2015, 45(12):1270-1276.
ZHANG K, YAN G Q, WANG J M, et al. Molecular epidemiological investigation of calf diarrhea coronavirus in parts of northern Xinjiang[J]. Chinese Veterinary Science, 2015, 45(12):1270-1276.(in Chinese)
[34] 邓飞, 陈弟诗, 陈斌, 等. 2020年四川省部分地区腹泻牛群牛冠状病毒感染情况调查[J]. 中国动物检疫, 2021, 38(9):13-16.
DENG F, CHEN D S, CHEN B, et al. Investigation on infection status of bovine coronavirus in diarrheal cattle in some regions of Sichuan province in 2020[J]. China Animal Health Inspection, 2021, 38(9):13-16.(in Chinese)
[35] 何琪富. 牦牛冠状病毒的分子检测及基因组研究[D]. 成都:西南民族大学, 2019.
HE Q F. Molecular detection and genomic characterization of bovine coronavirus in yak[D]. Chengdu:Southwest Minzu University, 2019.(in Chinese)
[36] COURA F M, FREITAS M D, RIBEIRO J, et al. Longitudinal study of Salmonella spp., diarrheagenic Escherichia coli, Rotavirus, and Coronavirus isolated from healthy and diarrheic calves in a Brazilian dairy herd[J]. Trop Anim Health Prod, 2015, 47(1):3-11.
[37] MAWATARI T, HIRANO K, IKEDA H, et al. Surveillance of diarrhea-causing pathogens in dairy and beef cows in Yamagata Prefecture, Japan from 2002 to 2011[J]. Microbiol Immunol, 2014, 58(9):530-535.
[38] PARK J, HAN D G, KIM S, et al. Prevalence of coronavirus from diarrheic calves in the Republic of Korea[J]. Asian Pac J Trop Biomed, 2018, 8(1):1-6.
[39] SEVINC TEMIZKAN S, ALKAN F. Bovine coronavirus infections in Turkey:molecular analysis of the full-length spike gene sequences of viruses from[LL]digestive and respiratory infections[J]. Arch Virol, 2021, 166(9):2461-2468.
[40] LOTFOLLAHZADEH S, MADADGAR O, MOHEBBI M R, et al. Bovine coronavirus in neonatal calf diarrhoea in Iran[J]. Vet Med Sci, 2020, 6(4):686-694.

河北省廊坊市牛主要病毒性腹泻病原感染状况检测及牛冠状病毒演化分析

Detection of Bovine Viral Diarrhea Pathogens and Evolution Analysis of Bovine Coronavirus in Langfang, Hebei

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	喻琦胜, 朱庆, 周群, 宋鑫, 张家祺, 陈涛云, 徐林, 张朝辉, 张斌. 杆状病毒表达系统表达BCoV纤突蛋白及其对小鼠的免疫原性[J]. 畜牧兽医学报, 2024, 55(2): 640-648.
[2]	黄江, 李闯, 崔月琦, 袁雪莹, 赵志诚, 刘宇, 周玉龙, 朱战波, 张泽财. 基于小鼠模型研究肠道菌群紊乱对BVDV易感性的影响[J]. 畜牧兽医学报, 2023, 54(8): 3466-3473.
[3]	张家祺, 甲呷格西, 周群, 宋鑫, 张斌. 宏基因组学技术分析四川省集约化猪场中仔猪腹泻粪便的病毒种群[J]. 畜牧兽医学报, 2023, 54(6): 2478-2486.
[4]	李易聪, 蒲飞洋, 冯茜莉, 汪梦竹, 赵泽阳, 张德荣, 马忠仁, 周建华. 牛病毒性腹泻病毒蛋白的免疫学特性以及相关疫苗研究进展[J]. 畜牧兽医学报, 2023, 54(4): 1381-1391.
[5]	何姝凡, 邹沅彤, 黄智兰, 李倩, 降措翁西, 岳华, 汤承, 刘杰. 牛腺病毒3型感染状况及其fiber轴区基因检测[J]. 畜牧兽医学报, 2023, 54(3): 1333-1340.
[6]	孙飞雁, 叶京飞, 魏宇, 王子贤, 张金玉, 邴丽媛, 孟婷婷, 王帅, 赵立峰, 孙亮, 郭利. 吉林省肉牛冠状病毒感染状况调查及分析[J]. 畜牧兽医学报, 2023, 54(2): 673-682.
[7]	陈俊贞, 权冉, 付强, 葛丽娟, 袁圆圆, 张成远, 李建林, 史慧君. 热休克蛋白HSP90B1影响牛病毒性腹泻病毒复制的研究[J]. 畜牧兽医学报, 2023, 54(2): 683-693.
[8]	王梦娇, 蒋倩, 马学军, 夏瑞阳, 郭雪萍, 孙磊, 钟旗, 马雪连, 姚刚. 新疆地区牛冠状病毒的分子流行病学调查[J]. 畜牧兽医学报, 2023, 54(12): 5125-5133.
[9]	张家祺, 贾浠宁, 周群, 宋鑫, 朱晨曦, 李格格, 张斌. 基于E2蛋白BVDV-1病毒样颗粒的构建及对小鼠的免疫效力评估[J]. 畜牧兽医学报, 2023, 54(12): 5143-5153.
[10]	郑如雯, 黄涛, 吴道义, 禹光美, 李芳芳, 隋鑫, 闵婷玉. 牛病毒性腹泻病毒LAMP-LFD检测方法的建立及初步应用[J]. 畜牧兽医学报, 2023, 54(11): 4745-4753.
[11]	史慧君, 董文丽, 郭妍婷, 袁圆圆, 陈俊贞, 杨莉, 冉多良, 付强. 细胞信号肽酶复合体亚基1与牛病毒性腹泻病毒复制的相关性分析[J]. 畜牧兽医学报, 2022, 53(6): 1870-1876.
[12]	李亚军, 茹毅, 郝荣增, 蒋成辉, 王伟, 张越, 张贵才, 刘华南, 卢炳州, 杨洋, 陶世宇, 杨锐, 宋向东, 陈娇, 余四九, 郑海学. CHO细胞表达牛病毒性腹泻病毒E2蛋白及免疫原性分析[J]. 畜牧兽医学报, 2022, 53(12): 4315-4324.
[13]	孙吉, 岳华, 汤承. 检测牛5种腹泻病毒的多重RT-PCR方法的建立及应用[J]. 畜牧兽医学报, 2022, 53(1): 209-218.
[14]	刘梦瑶, 王展慧, 吴浩, 谷月, 吴文学. 牛星状病毒、牛病毒性腹泻病毒1型、牛冠状病毒和牛轮状病毒四重实时荧光定量RT-PCR检测方法的建立[J]. 畜牧兽医学报, 2021, 52(7): 1942-1952.
[15]	高闪电, 王锦明, 田占成, 独军政, 王建东, 常惠芸, 关贵全, 李有全, 殷宏. 甘肃、青海和宁夏地区牛病毒性腹泻病毒E^rns基因的变异分析[J]. 畜牧兽医学报, 2021, 52(11): 3157-3164.