IGF1R和IGF2R在南江黄羊不同组织和肌细胞中的表达模式比较

doi:10.11843/j.issn.0366-6964.2019.04.003

摘要/Abstract

摘要：

为了探讨IGF1R和IGF2R基因在山羊不同组织及肌细胞中mRNA的表达模式，本研究采用qRT-PCR检测了南江黄羊妊娠期3个阶段（E45、E60和E105）的背最长肌及出生后5个时期（B3、B30、B60、B90和B120）的肌肉组织（背最长肌、臂三头肌）和内脏（心、肝、肺）中IGF1R和IGF2R的mRNA水平，以及骨骼肌卫星细胞（skeletal muscle satellite cells，SMSCs）增殖和分化过程中二者的表达模式。结果表明，在胎儿期的背最长肌中，IGF1R与IGF2R均呈现先上升后下降的表达趋势，其中IGF1R在E45和E60的表达量高于IGF2R（P<0.05，P>0.05），在E105的表达量低于IGF2R（P<0.01）。在出生后阶段，IGF1R在肺中表达量最高，肝中最低；相反，IGF2R在肺中表达量最低，在背最长肌中最高。随着羔羊的发育，IGF1R与IGF2R的表达量在心中呈现上升的趋势，在肝中呈现"下降-上升-下降"的表达变化，而在肺中两者的表达趋势相反（B90前）。在背最长肌和臂三头肌中，IGF1R的表达量呈现持续上升模式（B90前），IGF2R则呈现出"下降-上升-下降"的趋势。此外，IGF1R和IGF2R在SMSCs增殖阶段（24、48和72 h）呈现"下降-上升"的表达模式，分化早期（0~3 d）低表达而后期显著增加（分化5 d，P<0.05），随后下降。本研究获得了IGF1R和IGF2R基因在南江黄羊不同组织及SMSCs中的表达模式，初步揭示IGF1R在山羊肺中的高表达与肝中的低表达对器官的生长和功能维持具有关键作用，IGF1R与IGF2R协同促进其肌肉组织发育和肌细胞的增殖分化。

关键词: 南江黄羊, IGF1R, IGF2R, 组织, 卫星细胞, 基因表达

Abstract:

To investigate the mRNA expression patterns of IGF1R and IGF2R genes in different tissues and muscle cells of goats, real-time quantitative PCR was used to detect the mRNA levels of IGF1R and IGF2R in longissimus dorsi muscle at 3 prenatal stages (E45, E60 and E105), in muscle tissues (longissimus dorsi muscle, triceps brachii muscle) and visceral tissues (heart, liver and lung) at 5 postnatal stages (B3, B30, B60, B90 and B120) of Nanjiang Brown goats. Moreover, the expression patterns of IGF1R and IGF2R during skeletal muscle satellite cells (SMSCs) proliferation and differentiation was determined. The results showed that, in longissimus dorsi muscle of prenatal goats, the expression of IGF1R and IGF2R increased, then decreased. And the IGF1R expression level was higher than IGF2R in E45 and E60 (P<0.05, P>0.05), but lower than IGF2R in E105 (P<0.01). At the postnatal stages, the expression level of IGF1R was the highest in lung, and the lowest in liver; In contrast, the expression level of IGF2R was the lowest in lung but the highest in longissimus dorsi muscle. With the development of lambs, IGF1R and IGF2R exhibited ascending expression in heart, fluctuated (down-up-down) in liver, while the trends of IGF1R and IGF2R expression was reverse in lung (before B90). Furthermore, in longissimus dorsi and triceps brachii muscle, IGF1R showed increasing expression tendency(before B90), while IGF2R showed a down-up-down trend. Furthermore, IGF1R and IGF2R showed a up-down expression pattern during the proliferation stages (24, 48 and 72 h) of SMSCs, but was low at the early differentiation stages from 0 to 3 day, followed by significantly increased at 5 day (P<0.05), and then decreased. Expression patterns of IGF1R and IGF2R genes in different tissues and SMSCs of Nanjiang Brown goats were presented in this study. It is preliminarily reveal that high expression of IGF1R in lung and low expression in liver play a key role in organ growth and function maintenance, IGF1R synergizes with IGF2R to promote muscle tissue development and muscle cell proliferation and differentiation.

Key words: Nanjiang Brown goat, IGF1R, IGF2R, tissues, satellite cells, gene expression

中图分类号:

S827.2

占思远, 丁雪, 仲涛, 王林杰, 李利, 张红平. IGF1R和IGF2R在南江黄羊不同组织和肌细胞中的表达模式比较[J]. 畜牧兽医学报, 2019, 50(4): 701-711.

ZHAN Siyuan, DING Xue, ZHONG Tao, WANG Linjie, LI Li, ZHANG Hongping. Comparison of IGF1R and IGF2R Expression Patterns in Different Tissues and Muscle Cells of Nanjiang Brown Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 701-711.

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