Abstract: The goal of the present study was to prepare 3 D printed canine tissue engineering meniscus scaffold and evaluate its performance, explore the effect of in vitro chondrogenic induction time on the synthesis of extracellular matrix of canine bone marrow mesenchymal stem cells (BMSCs)-scaffold construction. After preparation of polycaprolactone (PCL) meniscus scaffold using 3D printing technology, the morphology and microstructure of the scaffold were observed by gross and scanning electron microscopy. The compressive modulus of the scaffold was determined by biomechanical test. The compatibility of scaffold and cell was evaluated by CCK-8 cytotoxicity experiment. Canine BMSCs were seeded into scaffold and induced by cartilage in vitro for 7, 14, 21 and 28 d, respectively. The growth of the cells on the scaffold was observed by inverted phase contrast microscopy. The effects of different induction time in vitro on matrix synthesis were analyzed and compared by measuring the glycosaminoglycan (GAG) content and the expression of type Ⅱ collagen at different induction time. The results showed that the PCL scaffold prepared by 3D printing had high degree of reduction of the natural anatomical shape of the meniscus, uniform pores and good inter-well connectivity, certain mechanical compressive properties and slow degradation rate,the number of cells increased. During the in vitro induction of BMSCs-scaffold construction, the cells proliferated continuously. The synthesis of GAG and type Ⅱ collagen increased with the induction time. The synthesis amount at 21 d was significantly higher than other induction time (P<0.05). The results proved that the PCL meniscus scaffold prepared by 3D printing has good physical and chemical properties and cell compatibility. It is expected to be used as a meniscus tissue engineering scaffold, and in vitro induction time has an important effect on the differentiation of BMSCs-scaffold construction into cartilage.