猪HMGA1基因的组织表达及其多态性与背膘厚的关联分析

doi:10.11843/j.issn.0366-6964.2019.07.003

摘要/Abstract

摘要： 旨在探究HMGA1基因在猪不同组织、不同日龄下不同品种中的表达谱，并对该基因编码区及3'UTR区的多态性与猪背膘厚进行关联分析。本研究利用实时荧光定量PCR检测大白猪HMGA1基因在7种组织的表达情况，及其在60、150和210日龄大白猪和民猪背部脂肪中的差异表达。对576头大白猪×民猪F2代资源群体的基因组DNA重测序进行HMGA1序列分析，筛选其编码区及3'UTR区SNPs，并以屠宰体重为协变量与背膘厚（第6~7肋）进行关联分析。结果表明，HMGA1 mRNA在大白猪不同组织中均有表达，且在组织间存在显著差异（P<0.05），其中在肺中的表达量最高，而在心和肌肉中微量表达。在60和150日龄时，HMGA1基因在民猪背部脂肪中的表达量显著高于大白猪（P<0.05）；210日龄时在两个猪种中的表达差异不显著（P>0.05）。通过对F2群体HMGA1序列分析，在编码区检测出13个单核苷酸多态性（single nucleotide polymorphism，SNP）位点，其中包含2个错义突变位点：g.3261G>A和g.5818G>A，且g.3261G>A位点与背膘厚显著关联（P<0.05）。在3'UTR区检测到3个SNPs，且位于小RNA（microRNA，miRNA）结合靶点，其中g.7217G>C和g.7280T>C与背膘厚显著关联（P<0.05）。研究结果表明，HMGA1作用于猪背部脂肪组织，且该基因g.3261G>A、g.7217G>C和g.7280T>C位点可作为猪背膘厚选育的潜在分子标记，为猪的分子育种奠定基础。

Abstract: The aim of this study was to explore the expression profile of HMGA1 gene in different tissues and at different ages in different pig breeds. Additionally, the association between the polymorphisms of the coding region and 3'UTR region and backfat thickness in pigs were analyzed. In this study, real-time quantitative PCR was used to detect the difference in mRNA expression of HMGA1 gene in 7 tissues of Large White, and in backfat of Large White and Minzhu at 60-, 150- and 210-day-old. The SNPs in the coding region and 3'UTR region of HMGA1 were detected by DNA re-sequencing in 576 Large White×Minzhu F2 generation resource populations. Then the association analysis between SNPs and backfat thickness (6-7 ribs) were performed with the slaughter weight as a covariate. The results showed that HMGA1 mRNA was widely expressed in various tissues of Large White, and significant difference was showed among different tissues (P<0.05). The expression of HMGA1 mRNA was the highest in the lung, but it was slightly expressed in the heart and muscle tissues. At 60- and 150-day-old, the expression of HMGA1 gene in backfat of Minzhu was significantly higher than that in the Large White (P<0.05), but the difference was not significant at 210-day-old between pig breeds(P>0.05). Thirteen single nucleotide polymorphism (SNP) sites were detected in the coding region of HMGA1, including 2 missense mutations sites:g.3261G>A and g.5818G>A, and the g.3261G >A was significantly associated with backfat thickness (P<0.05). Three SNPs were detected in the 3'UTR region as targets for the binding of microRNAs (miRNAs), the g.7217G>C and g.7280T>C were significantly associated with the backfat thickness(P<0.05). The above results indicate that HMGA1 gene has an effect on the backfat deposition of pigs, and the g.3261G>A, g.7217G>C and g.7280T>C loci can be used as potential molecular markers for the breeding of backfat thickness in pigs, which lay a foundation for the molecular breeding of pigs.

中图分类号:

S828.2

巩建飞, 岳静伟, 赵玲玲, 刘运鹏, 王立贤, 刘欣, 孙金海. 猪HMGA1基因的组织表达及其多态性与背膘厚的关联分析[J]. 畜牧兽医学报, 2019, 50(7): 1340-1346.

GONG Jianfei, YUE Jingwei, ZHAO Lingling, LIU Yunpeng, WANG Lixian, LIU Xin, SUN Jinhai. Tissue Expression Profiles of HMGA1 Gene in Pigs and Its Association with Backfat Thickness[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1340-1346.

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