ADIPOQ基因单体型与不同性别绵羊生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2018.04.021

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (4): 825-832.doi: 10.11843/j.issn.0366-6964.2018.04.021

ADIPOQ基因单体型与不同性别绵羊生长性状的关联分析

安清明^1,2, 周辉通^2,3, 刘秀², 李少斌², 罗玉柱², Jon G. Hickford^2,3*

1. 铜仁学院乌江学院, 铜仁 554300;
2. 甘肃农业大学动物科学技术学院, 甘肃草食动物生物技术重点实验室, 兰州 730070;
3. 林肯大学基因标记实验室, 林肯 7646

收稿日期:2017-10-09 出版日期:2018-04-23 发布日期:2018-04-19
通讯作者: 罗玉柱,教授,博士生导师,Tel:0931-7632483,E-mail:luoyz@gsau.edu.cn;Jon G. Hickford,教授,博士生导师,E-mail:Jon.hickford@lincoln.ac.nz
作者简介:安清明(1987-),男,甘肃景泰人,博士,副教授,主要从事动物遗传育种与繁殖研究,E-mail:anqingming2009@163.com。
基金资助:
甘肃省自然科学基金（1606RJZA074）；甘肃省农业生物技术项目（GNSW-2016-24）；贵州省教育厅自科项目（黔教合KY字[2017]089）；铜仁市科技计划项目（铜市科研[2016]18号-5）；铜仁学院博士科研启动项目（trxyDH1624）；甘肃农业大学自立项目（GSAU-ZL-2015-031）

Effect of Haplotypes of ADIPOQ on Growth Traits in Ovine with Different Genders

AN Qing-ming^1,2, ZHOU Hui-tong^2,3, LIU Xiu², LI Shao-bin², LUO Yu-zhu², JON G. Hickford^2,3*

1. Faculty of Wujiang, Tongren University, Tongren 554300, China;
2. Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
3. Gene-Marker Laboratory, Lincoln University, Lincoln 7646, New Zealand

Received:2017-10-09 Online:2018-04-23 Published:2018-04-19
Supported by:

摘要/Abstract

摘要：

旨在探讨ADIPOQ基因单体型对绵羊生长性状的性别差异，筛选出对绵羊生长性状具有性别差异的单体型，为提高绵羊生产效率及加速遗传改良进程提供依据。本研究以1 185只新西兰罗姆尼羊（公556只，母629只）为研究对象，利用PCR-SSCP方法检测不同性别绵羊中ADIPOQ基因变异及单体型分型情况。利用GLMs模型进行单体型与不同性别绵羊生长性状的关联分析。评估单体型对生长性状的表型影响效应，筛选出对绵羊生长性状具有性别差异的单体型。结果表明，绵羊ADIPOQ基因具有丰富的核苷酸变异及单体型构型，且单体型构型分布频率在不同性别绵羊中存在差异（本试验中单体型D₁-A₂及D₁-C₂只在母羔中检测到）；关联分析结果表明，ADIPOQ基因单体型对绵羊生长性状的影响存在性别特异性。结果显示，存在单体型A₁-A₂的公羔群体具有较低的断尾重（P=0.033）、断奶重（P=0.020）和断奶前生长速度（P=0.026）；其它单体型未发现对公羔生长性状具有显著性别特异性，且所有被检测的单体型对母羔生长性状均无显著影响。结果提示，ADIPOQ基因单体型构型分布频率在绵羊公、母羔中存在性别差异，且其对绵羊生长性状也具有性别特异性，存在单体型A₁-A₂的公羔生产性能较低，可以以此作为提高公羔生产性能的理论指导依据。

关键词: ADIPOQ基因, 单体型, 性别差异, 生长性状

Abstract:

The aims of this study were to analyze the effect of haplotypes of ADIPOQ gene on growth traits in male and female lambs, to filter haplotypes of ADIPOQ gene which had different effects on growth traits of male and female lambs, and to provide basis for improving the efficiency of production and breeding improvement. The mutations and haplotypes of ADIPOQ gene were detected in total 1 185 New Zealand Romney lambs (556 male and 629 female) by using PCR-SSCP method. The relationship between haplotypes and growth traits of male and female lambs was investigated using GLMs model. The effects of haplotypes of ADIPOQ on ovine growth traits were estimated and the haplotypes which had differents effects on growth traits of male and female lambs were identified. The results showed that ovine ADIPOQ gene had rich nucleotide mutations and haplotypes, and the frequencies of haplotypes were different in male and female lambs (haplotypes D₁-A₂ and D₁-C₂ were only detected in female lambs in this study). The associations analysis results showed that haplotypes of ovine ADIPOQ gene had different effects on growth traits of male and female lambs. In male lambs, the presence of A₁-A₂ was associated with decreased tailing weight (P=0.033), weaning weight (P=0.020) and pre-weaning growth rate (P=0.026). No associations were detected between the other haplotypes and growth traits of male lambs. No associations were found between the ADIPOQ haplotypes and growth traits of female lambs. Frequencies of ovine ADIPOQ haplotypes are different, and haplotypes may have different effects on growth traits of male and female lambs. The presence of A₁-A₂ is associated with decreased growth production, and may be used as a theoretical guidance for improving the growth performance of male lambs.

Key words: ADIPOQ gene, haplotype, gender-specific, growth traits

中图分类号:

S826.2

安清明, 周辉通, 刘秀, 李少斌, 罗玉柱, Jon G Hickford. ADIPOQ基因单体型与不同性别绵羊生长性状的关联分析[J]. 畜牧兽医学报, 2018, 49(4): 825-832.

AN Qing-ming, ZHOU Hui-tong, LIU Xiu, LI Shao-bin, LUO Yu-zhu, JON G. Hickford. Effect of Haplotypes of ADIPOQ on Growth Traits in Ovine with Different Genders[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 825-832.

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ADIPOQ基因单体型与不同性别绵羊生长性状的关联分析

Effect of Haplotypes of ADIPOQ on Growth Traits in Ovine with Different Genders

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