H19基因的单核苷酸多态性与猪生长性状的相关分析及其时空表达

doi:10.11843/j.issn.0366-6964.2016.05.003

摘要/Abstract

摘要：

本试验为了了解H19基因在猪骨骼肌生长发育过程中的作用，首先采用实时荧光定量PCR的技术检测了H19的组织分布以及在骨骼肌生长发育中的动态表达。然后通过混合池测序检测了H19 外显子和内含子区的单核苷酸多态位点（SNP），利用质谱在大白猪群体中进行SNPs位点基因型鉴定，并进行标记性状关联分析。H19基因在大白猪的心、肝、脾、肺、肾、小肠、胃、腿肌、背肌中均有表达，且在骨骼肌（腿肌、背肌）和肾中高表达。在骨骼肌发育中，H19主要是在胚胎期和出生后40 d之前有较高表达量，并且在胚胎期105 d达到最大值。对7个SNPs位点进行相关性分析，结果显示，RS15位点与达100 kg体重的校正日龄显著相关（P=0.046 2<0.05），RS20位点与100 kg的校正眼肌面积显著相关(P=0.009 5<0.01)。RS15与RS16紧密连锁，其单倍型与校正背膘厚显著相关（P=0.049 8<0.05）；RS17与RS18单倍型与达100 kg的校正眼肌面积显著相关（P=0.037 1<0.05）。结果表明H19基因参与猪骨骼肌发育调控，是猪产肉性状的候选基因。

Abstract:

An attempt was made to reveal the function of H19 gene in skeletal muscle development in pigs in this study.The distribution of H19 in different tissues and dynamic expression at different developmental stages in skeletal muscle were determined by Quantitative real-time PCR(qRT-PCR).The sites of single nucleotide polymorphisms (SNPs) in its exons and introns were identified by pool DNA sequencing，and individual SNPs were genotyped using mass spectrum in Large White pig population.The trait-marker association analysis was carried out between H19 genotypes and growth performance.The expression profiles showed that H19 RNA was expressed in all tissues detected，including heart，liver，spleen，lung，kidney，intestine，stomach and skeletal muscle (leg muscle and longissimus muscle)，with remarkably high expression in skeletal muscle and kidney.Meanwhile，H19 expression existed only before postnatal 40 days and reached the peak at 105 days in embryo period during the development of skeletal muscle.Correlation analysis between SNPs and traits showed the RS15 was significantly associated with corrected age of animals with body weight of 100 kg(P=0.046 2<0.05)，RS20 was associated with the corrected eye muscle area of animals with body weight of 100 kg(P=0.009 5<0.01)；The correlation analysis between haplotypes and traits shown that RS15 had a close linkage with RS16，and their haplotype was significantly correlated with corrected backfat thickness(P=0.049 8<0.05).RS17 was closely linked to RS18，and their haplotype was also significantly correlated with corrected eye muscle area of animals with body weight of 100 kg (P=0.037 1<0.05).In conclusion，H19 gene is involved in regulation of pig skeletal muscle development and is a candidate gene for meat traits.

中图分类号:

S828
S813.3

孙浩，王子帅，徐银学，唐中林. H19基因的单核苷酸多态性与猪生长性状的相关分析及其时空表达[J]. 畜牧兽医学报, 2016, 47(5): 870-881.

SUN Hao，WANG Zi-shuai，XU Yin-xue，TANG Zhong-lin. The Correlation between H19 Single Nucleotide Polymorphism and Pig Growth Characters and Its Spatio-temporal Expression[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 870-881.

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