TXNRD1基因多态性与乌珠穆沁绵羊生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2016.05.007

摘要/Abstract

摘要：

本研究旨在探讨TXNRD1多态性与乌珠穆沁绵羊生长性状之间的关联性，以期找到与乌珠穆沁绵羊生长有关的分子标记。采用DNA混池测序技术筛选TXNRD1基因外显子突变位点，并采用质谱分型技术检测343头乌珠穆沁绵羊群体突变位点的基因型，并对其单个SNP及多个SNPs位点之间的组合基因型与生长性状之间进行关联分析。结果发现：在该基因第1外显子处发现1个C→T的错义突变（RS10）；在第10外显子发现1个A→C同义突变（RS17）；在第12外显子处发现1个T→C同义突变（RS18）。χ²适合性检验表明，3个位点均处于Hardy-Weinberg平衡状态（P>0.05）。连锁不平衡和单倍型分析表明：RS10-RS17，RS10-RS18两个位点之间可能存在强连锁，RS17-RS18之间弱连锁。CAT是优势单倍型，其频率为0.356。关联分析发现，每个位点的3种基因型的个体在4月龄体重和胸围上均有显著差异（P＜0.05）。RS10位点3种基因型的个体在6月龄体重、体高、胸围及胸宽上差异显著（P<0.05），RS17位点3种基因型的个体在6月龄体高上差异显著（P<0.05），RS18位点上3种基因型的个体在6月龄体重、体高、体斜长和胸围上有显著性差异（P＜0.05）。组合基因型关联分析发现，不同多态位点之间的组合基因型在乌珠穆沁绵羊不同生长性状之间差异显著（P＜0.05）。综上所述，可以将TXNRD1基因的多态性位点作为分子标记，在乌珠穆沁绵羊的选育中进行探索和尝试。

Abstract:

The aim of this study was to investigate the association between the TXNRD1 gene polymorphisms and the sheep growth traits，and to find the molecular markers related to the growth traits of Ujumqin sheep.The variations in the exons of TXNRD1 gene were detected using DNA pool technology.Then Sequenom Mass-array technology was used to detect the genotypes at the mutation sites of the TXNRD1 gene in 343 Ujumqin sheep.The polymorphisms of TXNRD1 gene and its association with growth traits of Ujumqin sheep were analyzed by SPSS 22.0 software.The results showed that the C→T missense mutation (RS10) in exon 1，the A→C synonymous mutation（RS17） in exon 10 and the T→C synonymous mutation（RS18） in exon 12 were found，respectively.The x² test showed that all the 3 sites were in Hardy-Weinberg equilibrium(P>0.05).Then linkage disequilibrium and haplotypes were analyzed，the results showed that RS10-RS17，RS10-RS18 were strongly linked，while RS17-RS18 were weakly linked.The dominant haplotype was CAT with a frequency of 0.356.Assocition analysis suggested that 3 genotypes at each mutation site had significant difference in 4-month-age weight and chest girth (P<0.05).The 3 genotypes of RS10 showed significant difference in 6-month-age weight，body height，chest girth and chest width(P<0.05).At RS17 site，the 3 genotypes also had significant difference in 6-month-age body height (P<0.05).At RS18 site，there were significant difference among the 3 genotypes in 6-month-age weight，body height，body length and chest girth(P<0.05).Meanwhile，the different combined genotypes showed significant difference in various Ujumqin growth traits(P<0.05).Therefore，the results suggest that TXNRD1 mutation sites maybe markers related to the Ujumqin sheep growth traits and can be used as candidate markers in Ujumqin sheep molecular breeding.

中图分类号:

S826
S813.3

马晓萌，轩俊丽，王慧华，袁泽湖，吴明明，朱才业，刘瑞凿，魏彩虹，赵福平，张莉，杜立新. TXNRD1基因多态性与乌珠穆沁绵羊生长性状的关联分析[J]. 畜牧兽医学报, 2016, 47(5): 909-921.

MA Xiao-meng，XUAN Jun-li，WANG Hui-hua，YUAN Ze-hu，WU Ming-ming，ZHU Cai-ye，LIU Rui-zao，WEI Cai-hong，ZHAO Fu-ping，ZHANG Li，DU Li-xin. TXNRD1 Gene Polymorphisms and Its Association with Growth Traits in Ujumqin Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 909-921.

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https://www.xmsyxb.com/CN/Y2016/V47/I5/909

参考文献

［1］MUSTACICH D，POWIS G.Thioredoxin reductase［J］.Biochem J，2000，346 (Pt 1)：1-8.
［2］ZHANG R，AL-LAMKI R，BAI L，et al.Thioredoxin-2 inhibits mitochondria-located ASK1-mediated apoptosis in a JNK-independent manner［J］.Circ Res，2004，94(11)：1483-1491.
［3］KABUYAMA Y，KITAMURA T，YAMAKI J，et al.Involvement of thioredoxin reductase 1 in the regulation of redox balance and viability of rheumatoid synovial cells［J］.Biochem Biophys Res Commun，2008，367(2)：491-496.
［4］MATSUI M，OSHIMA M，OSHIMA H，et al.Early embryonic lethality caused by targeted disruption of the mouse thioredoxin gene［J］.Dev Biol，1996，178(1)：179-185.
［5］SMART D K，ORTIZ K L，MATTSON D，et al.Thioredoxin reductase as a potential molecular target for anticancer agents that induce oxidative stress［J］.Cancer Res，2004，64(18)：6716-6724.
［6］JIN R，GAO Y，ZHANG S，et al.Trx1/TrxR1 system regulates post-selected DP thymocytes survival by modulating ASK1-JNK/p38 MAPK activities［J］.Immunol Cell Biol，2015，93(8)：744-752.
［7］TSUJI P A，CARLSON B A，YOO M H，et al.The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways［J］.PLoS One，2015，10(4)：e0124487.
［8］ANESTÅL K，ARNÉR E S.Rapid induction of cell death by selenium-compromised thioredoxin reductase 1 but not by the fully active enzyme containing selenocysteine［J］.J Biol Chem，2003，278(18)：15966-15972.
［9］BIAGLOW J E，MILLER R A.The thioredoxin reductase/thioredoxin system：novel redox targets for cancer therapy［J］.Cancer Biol Ther，2005，4(1)：6-13.
［10］CEBULA M，SCHMIDT E E，ARNÉR E S.TrxR1 as a potent regulator of the Nrf2-Keap1 response system［J］.Antioxid Redox Signal，2015，23(10)：823-853.
［11］DAS S K，SHARMA N K，HASSTEDT S J，et al.An integrative genomics approach identifies activation of thioredoxin/thioredoxin reductase-1-mediated oxidative stress defense pathway and inhibition of angiogenesis in obese nondiabetic human subjects［J］.J Clin Endocrinol Metab，2011，96(8)：E1308-1313.
［12］BRENNAN K M，TERRY E N，MICHAL J J，et al.Body weight loss in beef cows：II.Increased antioxidant messenger ribonucleic acid levels in skeletal muscle but not erythrocyte antioxidant activity［J］.J Anim Sci，2009，87(9)：2867-2873.
［13］MITCHELL J，MORRIS A，DE BELLEROCHE J.Thioredoxin reductase 1 haplotypes modify familial amyotrophic lateral sclerosis onset［J］.Free Radic Biol Med，2009，46(2)：202-211.
［14］SOERENSEN J，JAKUPOGLU C，BECK H，et al.The role of thioredoxin reductases in brain development［J］.PLoS One，2008，3(3)：e1813.
［15］SUMIDA Y，NAKASHIMA T，YOH T，et al.Serum thioredoxin elucidates the significance of serum ferritin as a marker of oxidative stress in chronic liver diseases［J］.Liver，2001，21(5)：295-299.
［16］张成琰.硫氧还蛋白还原酶表达上调治疗非酒精性脂肪性肝病的实验研究［D］.太原：山西医科大学，2009.
ZHANG C Y.The experimental study of the treatment of non-alcoholic fatty liver disease through thioredoxin reductase up-regulated［D］.Taiyuan：Shanxi Medical University，2009.(in Chinese)
［17］DEKKERS J C，HOSPITAL F.The use of molecular genetics in the improvement of agricultural populations［J］.Nat Rev Genet，2002，3(1)：22-32.
［18］YAJIMA H，MOTOHASHI N，ONO Y，et al.Six family genes control the proliferation and differentiation of muscle satellite cells［J］.Exp Cell Res，2010，316(17)：2932-2944.
［19］LIU Y，CHU A，CHAKROUN I，et al.Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation［J］.Nucleic Acids Res，2010，38(20)：6857-6871.
［20］周明亮，张翔宇，綦松智，等.绵羊IGF-Ⅰ基因的表达及其SNPs与生长性状的相关分析研究［J］.畜牧兽医学报，2013，44(9)：1363-1370.
ZHOU M L，ZHANG X Y，QI S Z，et al.Expression and association between SNPs and growth traits of IGF-I gene in sheep［J］.Acta Veterinaria et Zootechnica Sinica，2013，44(9)：1363-1370.(in Chinese)
［21］LI Z，ZHANG Z，HE Z，et al.A partition-ligation-combination-subdivision EM algorithm for haplotype inference with multiallelic markers：update of the SHEsis (http：//analysis.bio-x.cn)［J］.Cell Res，2009，19(4)：519-523.
［22］HOWARD T D，WHITTAKER P A，ZAIMAN A L，et al.Identification and association of polymorphisms in the interleukin-13 gene with asthma and atopy in a Dutch population［J］.Am J Respir Cell Mol Biol，2001，25(3)：377-384.
［23］OROZCO G，HINKS A，EYRE S，et al.Combined effects of three independent SNPs greatly increase the risk estimate for RA at 6q23［J］.Hum Mol Genet，2009，18(14)：2693-2699.

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