郏县红牛SREBP-1c基因84 bp缺失突变的检测及其对6个生长性状的影响

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (10): 1225-1231.

郏县红牛SREBP-1c基因84 bp缺失突变的检测及其对6个生长性状的影响

黄永震¹，王居强²，马桂变³，张恩平¹，淮永涛¹，马亮¹，雷初朝¹，牛晖²，肖杰²，陈宏¹*

1. 西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室，杨凌 712100；2. 河南省肉牛工程技术研究中心，郑州 450003；3. 河南省平顶山市畜牧局，平顶山 467000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-10-25 发布日期:2010-10-25
通讯作者: 陈宏

Deletion Mutation of the Bovine SREBP-1c Gene of Jiaxian Red Cattle and Its Affecting on Growth Traits

HUANG Yong-zhen¹, WANG Ju-qiang², MA Gui-bian³, ZHANG En-ping¹, HUAI Yong-tao¹, MA Liang¹, LEI Chu-zhao¹, NIU Hui², XIAO Jie², CHEN Hong¹*

1. Key Laboratory of Agricultural Molecular Biology of Shanxi, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 2. Research Center of Cattle Engineering Technology of Henan, Zhengzhou 450003, China; 3. Pingdingshan Animal Husbandry Bureau, Pingdingshan 467000, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-10-25 Published:2010-10-25

摘要/Abstract

摘要： 旨在研究牛SREBP-1c基因内含子7区域84 bp缺失的遗传多态性及其对生长性状的影响。本试验以中国地方品种郏县红牛共计441头个体为实验动物，通过DNA测序技术和琼脂糖凝胶电泳方法检测该区域缺失突变的多态性。结果发现，在该突变位点检测到2种基因型：WW和WD型，等位基因W和D频率分别为0.865 1和0.134 9。He、Ne和PIC值都很低，说明在本突变位点遗传多态性不够丰富。该突变位点处于Hardy-Weinberg平衡状态（P>0.05）。该位点的多态性与郏县红牛6个生长性状指标关联分析显示，郏县红牛WD基因型个体在12、18、24月龄的体质量和胸围显著高于WW基因型个体（P<0.01或P<0.05），基因型WD个体在18、24月龄的体斜长显著高于WW基因型个体（P<0.05），基因型WD个体在18月龄的平均日增体质量显著高于WW基因型个体（P<0.01）。初步认为WD型是提高黄牛体质量和体尺指标性状的有利基因型。本研究结果表明，黄牛SREBP-1c基因内含子7区域84 bp缺失位点可作为郏县红牛生长性状的潜在分子育种标记，具有很大的研究价值。

关键词: SREBP-1c基因, 缺失突变, 郏县红牛, 生长性状

Abstract: The present study focused on screening the genetic variation within bovine SREBP-1c gene intron 7 and analyzing its effect on growth traits in 441 individuals belonging to Jiaxian Red cattle. In this study, the 84 bp deletion mutation of SREBP-1c gene was detected by using the DNA sequencing and agarose electrophoresis methods. The PCR products of SREBP-1c gene exhibited two genotypes WW and WD, and two alleles were revealed: W and D. Genotype WW was the predominant genotype and W was the predominant allele in the studied population. The frequencies of allelic W and D in Jiaxian Red cattle population were 0.865 1 and 0.134 9. Genetic indices (He, Ne and PIC) in this cattle population were presented low genetic diversity. The χ²test analysis demonstrated that the breed was in agreement with HardyWeinberg equilibrium (P>0.05). The association of the 84 bp deletion mutation of SREBP-1c gene with growth traits of Jiaxian Red cattle breed was analyzed. The animals with genotype WD had significantly higher body weight and heart girth than those with genotype WW (P<0.01 or P<0.05) at 12, 18 and 24 months. The animals with genotype WD had significantly greater body length than those with genotype WW (P<0.05) at 18 and 24 months. The individuals with genotype WD had significantly higher average daily gain than those with genotype WW (P<0.01) at 18 months. These results indicated that the growth stages and the selected traits should be comprehensively considered in the breeding practice of Jiaxian Red cattle. The genotype WD may have positive effect on growth traits. Therefore, these results suggest that the deletion mutation of bovine SREBP-1c gene may be a useful marker for growth traits in future marker-assisted selection programs in Jiaxian Red cattle.

Key words: SREBP-1c gene, deletion mutation, Jiaxian Red cattle, growth traits

黄永震;王居强;马桂变;张恩平;淮永涛;马亮;雷初朝;牛晖;肖杰;陈宏. 郏县红牛SREBP-1c基因84 bp缺失突变的检测及其对6个生长性状的影响[J]. 畜牧兽医学报, 2010, 41(10): 1225-1231.

HUANG Yong-zhen;WANG Ju-qiang;MA Gui-bian;ZHANG En-ping;HUAI Yong-tao;MA Liang;LEI Chu-zhao;NIU Hui;XIAO Jie;CHEN Hong . Deletion Mutation of the Bovine SREBP-1c Gene of Jiaxian Red Cattle and Its Affecting on Growth Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(10): 1225-1231.

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