SD雄鼠3种不同新蛋白构型GnRH去势抗原免疫学及生物学效果对比研究

doi:10.11843/j.issn.0366-6964.2019.04.007

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (4): 738-749.doi: 10.11843/j.issn.0366-6964.2019.04.007

SD雄鼠3种不同新蛋白构型GnRH去势抗原免疫学及生物学效果对比研究

韩兴发¹, 李俊丽¹, 曹晓涵¹, 储明星², 曾宪垠^1*

1. 四川农业大学生命科学学院原子能农业应用研究室, 雅安 625014;
2. 中国农业科学院北京畜牧兽医研究所, 农业部畜禽遗传资源与种质创新重点实验室, 北京 100193

收稿日期:2018-07-05 出版日期:2019-04-23 发布日期:2019-04-23
通讯作者: 曾宪垠,主要从事动物生理与生殖免疫调控研究,E-mail:xyzeng1966@163.com
作者简介:韩兴发(1986-),男,四川凉山人,博士生,主要从事动物生殖免疫调控研究,E-mail:xfhan2012@163.com
基金资助:
四川农业大学优秀博士论文培育基金（YB2014004）；四川农业大学双支计划（00770107）

Immunological and Biological Efficacy of Three-different Types of New GnRH-like Constructs in Male SD Rats

HAN Xingfa¹, LI Junli¹, CAO Xiaohan¹, CHU Mingxing², ZENG Xianyin^1*

1. Isotope Research Laboratory, College of Life Sciences, Sichuan Agricultural University, Ya'an 625014, China;
2. Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2018-07-05 Online:2019-04-23 Published:2019-04-23

摘要/Abstract

摘要：

旨在对比研究SD雄鼠3种不同新蛋白构型GnRH抗原的免疫学及生物学效果。本研究新蛋白构型GnRH抗原设计采用增加GnRH拷贝数和增大抗原分子量方法。以化学合成法合成G6K-GnRH并列体（G6K-GnRH-tandem，G6KT）作为新蛋白GnRH抗原制备的基本单元。将G6KT二聚化，形成抗原Ⅰ（G6KTD），将G6KT和G6KTD分别与卵清蛋白（OVA）偶联，分别形成抗原П（G6KT-OVA）及抗原Ⅲ（G6KTD-OVA）。试验分5组：完整对照组（不做任何处理，intact control）、外科阉割去势组（surgical castrates）及3种新蛋白构型GnRH免疫组（G6KTD、G6KT-OVA及G6KTD-OVA免疫组）。将（G6KTD、G6KT-OVA、G6KTD-OVA）3种不同新蛋白构型GnRH抗原配于Specol佐剂，分别免疫SD雄鼠，6周龄时初免，腿部肌肉注射1 mL乳化抗原（含相应新构型GnRH蛋白100 μg），8周后加免，注射剂量与方法同初次免疫，每试验组12只雄鼠。放免法及酶联免疫法测定血清抗体及生殖激素浓度变化，qPCR检测垂体-睾丸生殖相关基因mRNA表达变化。结果显示：1）SD雄鼠主动免疫3种不同新蛋白构型GnRH抗原后均能产生良好的抗体反应。2）G6KT-OVA及G6KTD-OVA免疫组仅初免就能诱发较高水平的抗体产生，并显著降低雄鼠血清LH、FSH、睾酮（T）及抑制素B（INHB）浓度（P<0.05）；试验结束时，该两种抗原免疫组雄鼠睾丸重量及体积均降低，精子发生完全被终止，垂体GnRHR、LHβ、FSHβ及睾丸LHR、FSHR、INHα、INβA及INHβB mRNA表达显著下调（P<0.05）。3）G6KTD免疫组12只雄鼠中，9只（75%）在加免后血清LH、FSH、T及INHB浓度显著降低（P<0.05），试验结束时睾丸重量及体积均显著降低（P<0.05），精子发生受到明显抑制（P<0.05）。综上表明：新蛋白构型G6KT-OVA和G6KTD-OVA具有极强的免疫原性，初次免疫就能达到抑制生殖激素分泌的目的，在作为单剂量GnRH疫苗方面具良好的开发前景；同时G6KTD也具有较强的免疫原性，可不与载体蛋白偶联单独作为GnRH去势抗原，继而避开GnRH分子与载体蛋白偶联所面临的GnRH抗原损失严重及与载体蛋白偶联的后续纯化等问题。本研究结果为研发高效GnRH去势疫苗及其大规模应用提供了理论参考。

Abstract:

The study was aimed to study the immunological and biological efficacy of 3-different types of GnRH-like constructs in male Sprague Dawley (SD) rats. The designing strategies for the new GnRH-like peptide constructs are based on:1) increasing the number of GnRH copy; 2) increasing the molecular weight of GnRH antigen. The new GnRH-like peptide constructs were designed based on a GnRH-tandem peptide, which was modified by relacing Gly at position 6 of the decapeptide with D-Lys (G6KT) to allow the conjugation of the GnRH peptide to ovalbumin (OVA). The first new GnRH-like construct was prepared by the dimerization of G6KT (G6KTD); the second and third were prepared by conjugating G6KT and G6KTD to OVA, respectively (G6KT-OVA and G6KTD-OVA). Sixty male SD rats at the age of 6 weeks, were randomly allocated to 5 groups (n=12):intact control (no treatment), surgically castrated or vaccinated against 100 μg peptide equivalent of G6KTD, G6KT-OVA and G6KTD-OVA in Specol adjuvant, respectively, at 6 weeks of age (with a booster 8 weeks later). Serum anti-GnRH antibody titers and reproductive hormone concentrations were assayed by RIA and ELISA, respectively. mRNA expressions of reproduction-related genes in pituitary-testicular axis were quantified using qPCR.The results shown that:1) active immunization against all of the 3 different new GnRH-like constructs could intrigue a good antibody responses in male SD rats; 2) Only with the first injection, active immunization against both G6KT-OVA and G6KTD-OVA intrigued a high antibody generation(P<0.05) and reduced serum concentrations of LH, FSH, testosterone and inhibin B(P<0.05), and further caused antrophy of testes with completely disrupted spermatogenesis and downregulated mRNA expressions of pituitary GnRHR, LHβ, FSHβ and testicular LHR, FSHR, INHα, INβA and INHβB (P<0.05) with the booster injection; 3) in contrast, the significant decrease of serum concentrations of LH, FSH, testosterone and inhibin B in rats actively immunized against G6KTD only occurred after the booster injection(P<0.05), but which still caused antrophy of testes with obviously disrupted spermatogenesis at decapitation (P<0.05) in 9 of 12 (75%) rats. In conclusion,based on those results, we concluded that both G6KT-OVA and G6KTD-OVA were highly efficacious GnRH antigen, which could be used as one-shot immunocastration vaccine in the furthure, whereas G6KTD itself was also effective, which might be used as GnRH antigen so as to avoid the drawbacks derived from the conjugation and purification processes of GnRH-carrier conjugates. Our findings promoted the further development and large scale commercial applications of GnRH vaccine.

中图分类号:

S814.1

韩兴发, 李俊丽, 曹晓涵, 储明星, 曾宪垠. SD雄鼠3种不同新蛋白构型GnRH去势抗原免疫学及生物学效果对比研究[J]. 畜牧兽医学报, 2019, 50(4): 738-749.

HAN Xingfa, LI Junli, CAO Xiaohan, CHU Mingxing, ZENG Xianyin. Immunological and Biological Efficacy of Three-different Types of New GnRH-like Constructs in Male SD Rats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 738-749.

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SD雄鼠3种不同新蛋白构型GnRH去势抗原免疫学及生物学效果对比研究

Immunological and Biological Efficacy of Three-different Types of New GnRH-like Constructs in Male SD Rats

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