血管紧张素转化酶2对Ang Ⅱ诱导的牛乳腺上皮细胞炎症损伤的缓解作用

doi:10.11843/j.issn.0366-6964.2019.07.015

摘要/Abstract

摘要： 以不同浓度血管紧张素Ⅱ（Ang Ⅱ）诱导牛乳腺上皮细胞（MAC-T）炎症损伤，探讨血管紧张素转化酶2（ACE 2）的变化及与Ang Ⅱ的相互作用。研究包括：ELISA检测细胞上清中细胞炎性因子的分泌或释放；Western blot检测细胞ACE 2和ACE的蛋白表达变化，并进行相关性分析；添加ACE 2活性蛋白对Ang Ⅱ诱导损伤的保护作用。结果显示：1） Ang Ⅱ处理MAC-T，细胞上清中促炎因子TNF-α、IL-6、IL-8含量显著（P<0.05）或极显著（P<0.01）增加，抗炎因子IL-10含量显著降低（P<0.05）；2）不同浓度Ang Ⅱ处理细胞后，ACE 2蛋白表达均有降低，10^-6 mol·L^-1 Ang Ⅱ处理后显著降低（P<0.05）；ACE蛋白表达结果相反；ACE 2与Ang Ⅱ浓度之间存在显著负相关；3）添加外源性ACE 2活性蛋白，细胞上清中TNF-α、IL-6和IL-8浓度均有一定程度的下降，IL-10浓度升高。本研究表明ACE 2/ACE轴的失衡是Ang Ⅱ诱导细胞炎性损伤的主要原因。高水平Ang Ⅱ可激活炎症因子促进炎症反应，是促进炎症反应的主要介质，ACE 2可以通过降解Ang Ⅱ，抑制其对炎症反应的上调效应。

Abstract: Inflammatory damage of bovine mammary epithelial cells (MAC-T) was induced by different concentrations of angiotensin Ⅱ (Ang Ⅱ), and the changes of angiotensin-converting enzyme 2 (ACE 2) and the interaction with Ang Ⅱ were explored. The research includes:Detecting the secretion or release of inflammatory factors in cell supernatant by ELISA; Western blot analysis of protein expression changes of ACE 2 and ACE, and correlation analysis; The protective effect of ACE 2 active protein on Ang Ⅱ induced injury. Results were as follows:1) The levels of pro-inflammatory factors TNF-α, IL-6 and IL-8 in the supernatant of bovine MAC-T cells in the Ang Ⅱ treatment were significantly (P<0.05) or very significantly increased (P<0.01), the anti-inflammatory factor IL-10 content was significantly reduced (P<0.05); 2) Treatment with different concentrations of Ang Ⅱ, the expression of ACE 2 protein was decreased, and the 10^-6 mol·L^-1 Ang Ⅱ treatment group was significantly decreased (P<0.05); the expression of ACE protein was reversed. There was a significant negative correlation between ACE 2 and Ang Ⅱ concentrations; 3) After adding exogenous ACE 2 active protein, the results showed that the concentration of TNF-α, IL-6 and IL-8 in the supernatant of the cells decreased to some extent, and the concentration of IL-10 increased. The imbalance of the ACE 2/ACE axis is the main cause of inflammatory injury induced by Ang Ⅱ. The conclusion showed that high level of Ang Ⅱ can activate inflammatory factors to promote inflammatory response and is the main mediator of inflammatory response. ACE 2 can inhibit the up-regulation of inflammatory response by degrading Ang Ⅱ.

中图分类号:

S852.2

朱斌, 刘小倩, 刘颖, 纪晓霞, 张源淑. 血管紧张素转化酶2对Ang Ⅱ诱导的牛乳腺上皮细胞炎症损伤的缓解作用[J]. 畜牧兽医学报, 2019, 50(7): 1458-1465.

ZHU Bin, LIU Xiaoqian, LIU Ying, JI Xiaoxia, ZHANG Yuanshu. Angiotensin-converting Enzyme 2 on Ang Ⅱ-induced Mitigation of Inflammatory Injury in Bovine Mammary Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1458-1465.

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