不同基因型猫嵌杯状病毒的分离鉴定与致病性比较

doi:10.11843/j.issn.0366-6964.2025.08.048

Abstract

Abstract:

This study aimed to analyze the genetic variation and pathogenic characteristics of feline calicivirus (FCV) and successfully isolated and identified five prevalent FCV strains. A total of 127 clinical samples were tested, yielding 31 nucleic acid-positive samples (positivity rate: 24.4%). Five viral strains were isolated via cell culture. Results were as follows: Among them, four strains (e.g., HN78) were classified as FCV genotype Ⅰ, and one strain (HN77) as genotype Ⅱ. Electron microscopy revealed typical spherical, non-enveloped viral particles, and VP1 gene sequencing indicated 75.4%-75.65% homology among the strains. Animal challenge experiments confirmed that both genotype Ⅰ (HN78) and genotype Ⅱ (HN77) induced typical clinical symptoms, though pathogenic features differed between genotypes. This study provides the first systematic comparison of the biological characteristics of FCV genotypes Ⅰ and Ⅱ in China, offering critical viral strain resources and a theoretical foundation for vaccine development and clinical prevention strategies.

Key words: feline calicivirus (FCV), genetic characteristics, variations, isolated strains, gene sequencing

CLC Number:

S852.659.6

LI Bowen, WANG Shujuan, HU Yufeng, WANG Yibing, GAN Xueqiang, CHEN Yanyan, WANG Dongfang, MA Zhenyuan, ZHAO Xueli, YAN Ruoqian. Isolation and Identification of Feline Calicivirus and Analysis of Important Nucleotide Sequences[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(8): 4112-4119.

Figures/Tables 3

Table 1

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References 9

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序号 No.	毒株 Reference strains	登录号 GenBank ID	来源地 Locality	分离时间 Time
1	SH/2014VSD	KT000003	中国上海(国内已通过应急评价疫苗株)	2014年
2	FCV-255	KM111171	美国疫苗株	2014年
3	F9	M86379	英国疫苗株	1992年
4	XH	KX371572	中国黑龙江	2016年
5	WZ-1	KX371573	中国黑龙江	2016年
6	CH-JL1	KJ495728	中国吉林	2014年
7	CH-JL4	KT206207	中国吉林	2015年
8	SJ17	PP061538	中国上海	2023年
9	SH	KP987265	中国上海	2015年
10	FCV45-BJ-2023	PP554921	中国北京	2023年
11	BJ-231	MW088954	中国北京	2021年
12	SX2	MT759584	中国山西	2020年
13	GX01-13	KT970059	中国广西	2015年
14	HZ-1	MT680938	中国浙江	2020年
15	SMU-B5-2020	MW194990	中国四川	2020年
16	KN	OR472393	中国江苏	2023年
17	5789	JX519210	美国	2013年
18	VS-FCV-Ari	DQ910794	美国	2007年
19	BD	BD338978	美国	2003年
20	GE	DQ424892	德国	2006年

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Isolation and Identification of Feline Calicivirus and Analysis of Important Nucleotide Sequences

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