Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (3): 1366-1375.doi: 10.11843/j.issn.0366-6964.2025.03.035
• Preventive Veterinary Medicine • Previous Articles Next Articles
YU Xinya1(
), HE Haijian2, WANG Lei1, NI Yuchen1, DU Jing1, ZHOU Yingshan1, DONG Wanyu1,*(), WANG Xiaodu1,*()
Received:2024-04-17
Online:2025-03-23
Published:2025-04-02
Contact:
DONG Wanyu, WANG Xiaodu
E-mail:1131440070@qq.com;wanyudong@zafu.edu.cn;xdwang@zafu.edu.cn
CLC Number:
YU Xinya, HE Haijian, WANG Lei, NI Yuchen, DU Jing, ZHOU Yingshan, DONG Wanyu, WANG Xiaodu. Effect of lncRNA 18850 on Porcine Epidemic Diarrhea Virus Replication[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1366-1375.
Table 1
Sequences of lncRNA real-time fluorescence quantitative PCR primers"
| 基因 Gene | 引物序列(5′→3′) Primer sequences(5′→3′) | 长度/bp Length(bp) |
| lncRNA 18850 | GTCCTGCCTCTTCTTTCCATAAG | 137 |
| GAGTTCCAAGTGGCTATCTACAA | ||
| CCN2 | TGGAAGAGAACATTAAGAAGGGCA | 190 |
| TCAGGGCACTTGAACTCCAC | ||
| TFPI2 | TGCCAACGTGACTCGCTATT | 141 |
| ATTGGCCTCAACCCTTTTGC | ||
| NOTCH3 | GGTGGAAGAGCTTATCGCCA | 166 |
| CAGAGGGGTTTCTTCCTTGCT | ||
| CXCL8 | AAACCACCGGAAGGAACCAT | 113 |
| GGCAAAACTGCACCTTCACA | ||
| PTN | TGTTCACCTAACTGCCGTAGCA | 101 |
| TGGTACTGTTGAGCCTGCAT | ||
| RRAD | GTATGGTGGGAGACGGATGG | 134 |
| GTCTGTCCACGACCATGAGG | ||
| ITGA7 | CAAGTATGAAGTCACGGTC | 133 |
| GTCCACATCCAGGTAGAG | ||
| GAPDH | GGATGCAGGGATGATGTTC | 374 |
| TGCACCACCAACTGCTTAG |
Fig. 1
Changes in lncRNA in Vero-E6 cells infected with PEDV A. lncRNA expression profiles at 24 hours post-PEDV infection; B. Number of differentially expressed lncRNAs at various time points; C. Top ten lncRNAs with significant differential expression; D. Venn plots of significantly changed lncRNAs at different time points; E. qPCR detection of lncRNA 18850 expression at different time points of PEDV infection. **. P < 0.01"
Fig. 2
Effect of lncRNA 18850 overexpression on PEDV replication in Vero-E6 cells A. Expression levels of lncRNA 18850 overexpression at different time points of; B. Effect of lncRNA 18850 overexpression on PEDV-induced CPE; C. indirect immunofluorescence detection of the effect of lncRNA 18850 overexpression on PEDV N protein expression; D. Western blot detection of PEDV N protein expression in cells overexpressing lncRNA 18850; E. Gray scale analysis of PEDV N protein expression in D plot; F. TCID50 determination of viral titers in PEDV-infected cells overexpressing lncRNA 18850; G. qPCR determination of viral RNA copy number in PEDV-infected cells overexpressing lncRNA 18850. *. P < 0.05; ***. P < 0.001"
Fig. 3
lncRNA 18850 regulates multiple signaling pathways and genes A. Differential gene expression profiles in Vero-E6 cells overexpressing lncRNA 18850; B. Heatmap analysis of the number of up-and down-regulated genes in lncRNA 18850 overexpressing Vero-E6 cells (P < 0.05); C. Volcano plot of differentially genes. Red indicates up-regulated significantly differentially expressed genes, blue indicates down-regulated significantly differentially expressed genes, and grey indicates non-significant genes; D. Changes in differentially expressed gene from transcriptome sequencing results; E. qPCR detection of changes in gene shown in D plot after overexpression of lncRNA 18850. NC represents the control group. *. P < 0.05; **. P < 0.01; ***. P < 0.001; ns. No significant difference."
Fig. 4
Signaling pathways and genes regulated by lncRNA 18850 A. KEGG hierarchical bar chart. The horizontal coordinates represents the number of differential genes in each pathway, and the color indicates the KEGG level 1 classification; B. KEGG enrichment bar chart. The vertical coordinate is the pathway name and the horizontal coordinate is the-lg P from GO term enrichment analysis; C. Co-expression and trans relationships plot. Target genes were screened using Pearson correlation test and RIsearch-2.0. Red indicates up-regulated genes, blue indicates down-regulated genes, with darker colors indicating higher significance of differential expression, Grey solid lines indicate co-expression and trans relationship between the gene and lncRNA 18850, while black solid lines indicate protein interaction relationships between genes"
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