聚苯乙烯纳米塑料对公鸭睾丸的损伤作用

doi:10.11843/j.issn.0366-6964.2025.02.040

Abstract

Abstract:

In order to reveal the male reproductive toxicity of polystyrene nanoplastics (PS-NPs), this study investigated the effects of PS-NPs on duck testicular tissue using male Shaoxing ducks. In this study, 32 Shaoxing ducks were randomly divided into 4 groups(8 ducks in each group), namely, blank Control group, L-NPs group, M-NPs group, and H-NPs group, and were fed with PS-NPs at dietary concentrations of 0, 1, 10 and 100 mg·kg^-1. After 45 days, the body weight of the ducks were weighed, the testes of the ducks were collected, testis coefficients were calculated, the pathological changes of testis tissues of each group were observed by HE staining. The testicular tissues were tested for malondialdehyde (MDA) content and the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) by kit. The expression levels of oxidative damage-related proteins Nrf2, NQO1 and HO-1 were detected by Western blot. The mRNA levels of inflammation-related genes TNF-α and IL-6 were detected by real-time fluorescent quantitative PCR. The results showed that the testicular coefficient was significantly reduced in the H-NPs group with the increase of PS-NPs treatment concentration compared with the control group (P<0.05). The pathological results showed that the interstices between the convoluted seminiferous tubules were continuously enlarged, the number of interstitial cells was gradually reduced, and the structure of convoluted tubule wall was blurred. The testicular tissues of the M-NPs group and H-NPs group had a significant (P<0.05) and highly significant (P<0.01) increase in the content of MDA. The activity of GSH, SOD and CAT decreased significantly (P<0.05) and very significantly (P<0.01). Western blot results showed that the protein expression levels of Nrf2, NQO1 and HO-1 present a trend of significant (P<0.05) or highly significant decrease (P<0.01) in the M-NPs group and H-NPs group. The real-time fluorescence quantitative PCR results showed that the mRNA transcript levels of TNF-α and IL-6 genes were significantly (P<0.05) and highly significant increased (P<0.01). The results showed that PS-NPs could cause morphological and structural changes, inflammation and oxidative stress in the testicular tissues of Shaoxing ducks. The degree of damage was positively correlated with the dose of PS-NPs exposure. The inflammatory response caused by PS-NPs may be closely related to the Nrf2/HO-1 signaling pathway.

Key words: polystyrene nanoplastics, duck, testicle, oxidative stress, inflammation

CLC Number:

GU Yayi, XIA Sugan, LIU Pengli, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Damaging Effect of Polystyrene Nanoplastics on the Testicles of Male Ducks[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(2): 925-933.

Figures/Tables 6

Table 1

Fig. 1

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References 28

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基因 Genes	引物序列(5′→3′) Primer sequences
TNF-α	ACAGGACAGCCTATGCCAAC
	ACAHHAAHHHCAACACATCT
IL-6	TGGCTTCGACGAGGAGAAA
	AGCTGAATCTGGGATGACCAC
β-Actin	ATGTCGCCCTGGATTTCG
	CACAGGACTCCATACCCAAGAAT

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Damaging Effect of Polystyrene Nanoplastics on the Testicles of Male Ducks

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