基于斜率比法评价断奶仔猪对高温烧结法磷酸三钙的相对生物学利用率

doi:10.11843/j.issn.0366-6964.2025.01.025

Abstract

Abstract:

This study was conducted to determine the relative bioavailability of tricalcium phosphate (TCP), which is produced by high-temperature sintering method, by using dicalcium phosphate (DCP) as the reference, and talking the growth performance, dietary digestibility, serum and bone indexes of weaned piglets as indicators. The experiment conducted a 2×3 factorial arrangement experimental design, DCP and TCP were added into basal diet at 3 phosphorus supplemental levels, which were 0.08%, 0.16% and 0.24%, respectively, and two kinds of phosphates shared one control treatment which fed the basal diet. One hundred and forty Duroc×Large White×Yorkshire weaned piglets with similar body weight were randomly allocated into 7 groups with 4 replicates in each group and 5 piglets in each replicate. The experimental period lasted for 38 days including a 3-day adaptation period and a 35-day normal test period. The results showed that the phosphorus source and supplemental level and their interaction had no significant effects on growth performance of weaned piglets (P>0.05). The level of phosphorus supplementation significantly affected the apparent digestibility of dietary calcium and phosphorus, phosphorus content in tibia and metacarpal bones, and fracture strength of femur and metacarpal bones in piglets and presented a dose effect with the increase of phosphorus (P < 0.05). Compared with DCP, TCP significantly increased the apparent digestible phosphorus intake, femoral fracture strength, and metacarpal phosphorus content of piglets (P < 0.05). Considering the sensitive indicators such as dietary apparent digestibility of calcium and phosphorus, apparent digestible phosphorus, femoral and metacarpal strength, as well as metacarpal calcium and phosphorus contents, the average relative bioavailability of TCP is 109% with a reference to the bioavailability of DCP (100%). In conclusion, the bioavailability of phosphorus in TCP is better than that in DCP, which is beneficial for promoting bone calcium and phosphorus deposition and enhancing the bone strength.

Key words: weaned piglet, tricalcium phosphate, dicalcium phosphate, relative bioavailability, slope ratio

CLC Number:

S816.32

FAN Dingkun, ZHANG Tao, JIAO Shuai, LU Wei, FU Yuze, YANG Hong, TU Yan, SHI Lingyuan, ZHANG Naifeng. Evaluation of Relative Bioavailability of Tricalcium Phosphate Produced by High-temperature Sintering Method in Weaned Piglets Based on Slope Ratio Method[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 269-280.

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References 33

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原料Ingredient	含量Content	营养水平Nutrient level³⁾	含量Content
玉米Corn	60	干物质DM	88.98
豆粕Soybean meal	33.5	代谢能/(MJ·kg^-1) ME	12.46
预混料Premix¹⁾	4	粗蛋白质CP	19.97
矿物质预混剂Mineral premix²⁾	2.5	粗脂肪EE	0.63
合计Total	100	中性洗涤纤维NDF	15.60
		酸性洗涤纤维ADF	8.78
		钙Ca	0.11
		总磷TP	0.35

项目 Item	对照组CON	磷酸氢钙DCP			磷酸三钙TCP
项目 Item	0	0.08%	0.16%	0.24%	0.08%	0.16%	0.24%
原料Ingredient
磷酸三钙TCP	/	/	/	/	17.6	35.2	52.8
磷酸氢钙DCP	/	18	36	54	/	/	/
石粉Limestone	35.6	34	33.2	32.8	29.2	24	19.2
沸石粉Zeolite	64.4	48	30.8	13.2	53.2	40.8	28
合计Total	100	100	100	100	100	100	100
营养成分Nutrient
日粮钙Dietary Ca	0.60	0.64	0.69	0.74	0.64	0.69	0.74
日粮磷Dietary P	0.35	0.43	0.51	0.59	0.43	0.51	0.59

项目 Item	磷水平/% P levels	初重/kg IBW	末重/kg FBW	日增重/(g·d^-1) ADG	采食量/(g·d^-1) DMI	料重比 F/G
对照组CON	0	16.75	32.97	569.83	1 214.53	2.14
磷酸三钙 TCP	0.08	15.65	34.75	606.15	1 225.64	2.03
	0.16	15.88	33.06	549.93	1 184.09	2.20
	0.24	16.36	35.20	624.60	1 283.01	2.07
磷酸氢钙 DCP	0.08	14.25	33.05	553.47	1 177.69	2.15
	0.16	15.54	33.07	550.53	1 233.64	2.26
	0.24	14.72	33.65	566.57	1 172.30	2.08
	SEM	0.82	1.59	37.56	41.53	0.11
磷源 P sources	磷酸三钙TCP	15.96	34.34^a	593.56	1 230.92	2.10
磷源 P sources	磷酸氢钙DCP	14.84	33.26^b	556.85	1 194.54	2.16
SEM	0.51	0.93	34.54	43.73	0.07
磷水平 P levels	0.08	14.95	33.90	579.81	1 201.67	2.09
	0.16	15.71	33.07	550.23	1 208.86	2.23
	0.24	15.54	34.43	595.59	1 227.66	2.07
	SEM	0.62	1.12	23.68	29.98	0.08
P值 P-value	磷源P source	0.132 6	0.037 6	0.080 9	0.192 9	0.250 6
	磷水平P level	0.669 0	0.628 6	0.438 5	0.759 8	0.403 9
	磷源×磷水平 P source× P level	0.737 3	0.449 1	0.487 3	0.147 0	0.856 7

项目 Item	磷水平/% P level	表观全消化道消化率/% ATTD			表观可消化磷/(g·kg^-1 DMI) ADP
项目 Item	磷水平/% P level	干物质DM	钙Ca	磷P	表观可消化磷/(g·kg^-1 DMI) ADP
对照组CON	0	87.41^a	44.66	39.42	1.61
磷酸三钙 TCP	0.08	87.96^d	47.63	45.32	2.37
磷酸三钙 TCP	0.16	92.55^b	51.39	46.78	2.85
0.24	89.14^c	53.56	50.83	3.96
磷酸氢钙 DCP	0.08	92.18^b	46.60	43.63	2.23
	0.16	92.21^b	50.53	44.65	2.85
	0.24	94.17^a	54.13	50.36	3.59
	SEM	0.30	1.63	1.08	0.09
磷源 P sources	磷酸三钙TCP	89.88	50.86	47.59	3.06^a
	磷酸氢钙DCP	92.85	50.42	46.27	2.88^b
	SEM	0.21	0.93	0.55	0.05
磷水平/% P levels	0.08	90.07	47.11^b	44.52^b	2.29^c
	0.16	92.38	50.96^a	45.69^b	2.85^b
	0.24	91.66	53.84^a	50.58^a	3.77^a
	SEM	0.25	1.12	0.66	0.06
P值 P-value	磷源P source	＜0.000 1	0.969 6	0.075 1	0.015 1
	磷水平P level	＜0.000 1	0.002 5	＜0.000 1	＜0.000 1
	磷源×磷水平 P source× P level	＜0.000 1	0.790 3	0.658 5	0.123 6

项目 Item	磷水平/% P level	碱性磷酸酶/ (U·L^-1) ALP	磷/(mmol·L^-1) P	钙/(mmol·L^-1) Ca	甲状旁腺素/ (pg·mL^-1) PTH	降钙素/ (pmol·L^-1) CT
对照组CON	0	273.73^a	3.33^ab	3.81	23.23	2.17^b
磷酸三钙 TCP	0.08	199.08^c	3.02^ab	4.10	22.06	2.61^a
	0.16	287.31^a	3.32^a	3.33	21.70	2.44^ab
	0.24	197.16^c	2.99^ab	4.35	22.59	2.22^ab
磷酸氢钙 DCP	0.08	271.89^ab	3.38^a	3.06	20.28	1.99^b
	0.16	224.35^bc	2.86^b	2.50	24.02	2.19^ab
	0.24	229.02^bc	3.24^ab	4.37	22.53	2.66^a
	SEM	19.71	0.14	0.75	2.12	0.17
磷源 P source	磷酸三钙TCP	227.85	3.11	3.93	22.12	2.42
	磷酸氢钙DCP	241.75	3.16	3.31	22.28	2.28
	SEM	11.38	0.08	0.43	0.91	0.10
磷水平/% P level	0.08	235.49	3.20	3.58	21.17	2.30
	0.16	255.83	3.09	2.91	22.86	2.31
	0.24	213.09	3.12	4.36	22.56	2.44
	SEM	13.94	0.10	0.53	1.12	0.13
P值 P-value	磷源P source	0.394 6	0.635 1	0.317 9	0.903 1	0.332 5
	磷水平P level	0.112 5	0.695 1	0.170 4	0.531 2	0.673 4
	磷源×磷水平 P source×P level	0.005 4	0.010 9	0.756 2	0.437 4	0.018 7

Evaluation of Relative Bioavailability of Tricalcium Phosphate Produced by High-temperature Sintering Method in Weaned Piglets Based on Slope Ratio Method

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项目Item	磷添加水平/% P level	骨骼断裂强度/kg Bone fracture strength
		股骨Femur	胫骨Tibia	掌骨Metacarpal
		对照组CON	0	56.08	58.18	18.03
磷酸三钙 TCP	0.08	57.92	59.19	16.65
	0.16	60.79	60.58	18.58
	0.24	65.68	60.03	18.23
磷酸氢钙 DCP	0.08	53.70	59.99	17.74
	0.16	56.51	59.68	18.63
	0.24	67.00	59.17	18.36
	SEM	1.50	1.12	0.35
磷源 P source	磷酸三钙TCP	61.46	59.93	17.82
	磷酸氢钙DCP	59.07	59.61	18.25
	SEM	0.86	0.64	0.20
磷水平/% P level	0.08	55.80^b	59.59	17.20^b
	0.16	58.65^b	60.13	18.61^a
	0.24	66.34^a	59.60	18.30^a
	SEM	1.06	0.79	0.25
P值 P-value	磷源P source	0.059 8	0.729 5	0.150 0
	磷水平/% P level	＜0.000 1	0.859 6	0.000 9
	磷源×磷水平P source level	0.116 5	0.699 0	0.275 2

因变量 Dependent variable	截距 Intercept	回归系数 Regression coefficient		R²	P值 P-value	相对生物学利用率(SE)/% RBV(SE)		P值 P-value
因变量 Dependent variable	截距 Intercept	DCP	TCP	R²	P值 P-value	DCP	TCP	P值 P-value
钙表观消化率/% Ca apparent digestibility	36.08	2.377 3	2.256 1	0.533 7	0.001 1	100	96(8.61)	0.000 5
磷表观消化率/% P apparent digestibility	34.53	1.912 0	2.030 0	0.680 8	＜0.000 1	100	107(8.59)	< 0.000 1
表观可消化磷/(g·d^-1) Apparent digestible phosphorus	-0.786 9	0.593 8	0.601 3	0.963 8	＜0.000 1	100	103(1.56)	< 0.000 1
股骨强度/kg Femur strength	35.12	3.872 9	4.066 6	0.555 4	< 0.000 1	100	106(5.82)	< 0.000 1
掌骨强度/kg Metacarpal strength	15.44	0.443 9	0.374 6	0.273 9	0.016 3	100	84(11.9)	0.038 8
掌骨钙/% Metacarpal calcium	1.285 9	0.190 8	0.295 7	0.907 1	< 0.000 1	100	155(10.0)	< 0.000 1
掌骨磷/% Metacarpal phosphorus	0.882 0	0.038 3	0.042 4	0.521 0	0.000 1	100	111(8.43)	< 0.000 1
TCP相对生物学利用率 TCP relative biological availability						100	109(8.40)

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项目 Item	磷水平/% P level	股骨Femur			胫骨Tibia			掌骨Metacarpal
项目 Item	磷水平/% P level	灰分Ash	钙Ca	磷P	灰分Ash	钙Ca	磷P	灰分Ash	钙Ca	磷P
对照组CON	0	39.07	2.72	1.29^b	40.37	3.20	1.27	40.16	2.01^d	1.04
磷酸三钙 TCP	0.08	42.89	2.68	1.41^a	40.04	3.44	1.19	41.05	2.70^c	1.09
	0.16	41.82	3.01	1.35^ab	39.59	3.29	1.30	40.47	3.25^b	1.19
	0.24	40.74	3.28	1.25^b	38.76	3.49	1.21	40.49	3.61^a	1.19
磷酸氢钙 DCP	0.08	40.75	3.03	1.26^b	39.99	3.15	1.23	42.95	2.29^d	1.04
	0.16	39.98	3.23	1.28^b	39.47	3.33	1.31	41.38	2.36^d	1.17
	0.24	41.61	2.74	1.40^a	40.08	2.93	1.33	39.79	2.79^c	1.15
	SEM	1.15	0.29	0.05	0.76	0.18	0.04	0.69	0.11	0.02
磷源 P source	磷酸三钙TCP	41.82	2.99	1.34	39.47	3.41	1.24	40.67	3.18	1.16^a
	磷酸氢钙DCP	40.78	3.00	1.31	39.85	3.14	1.29	41.37	2.48	1.12^b
	SEM	0.64	0.17	0.02	0.41	0.10	0.02	0.36	0.02	0.01
磷水平/% P level	0.08	41.82	2.85	1.34	40.02	3.30	1.21^b	42.00^a	2.49	1.06^b
	0.16	40.90	3.12	1.32	39.53	3.31	1.30^a	40.92^ab	2.80	1.18^a
	0.24	41.17	3.01	1.33	39.42	3.21	1.27^ab	40.14^b	3.20	1.17^a
	SEM	0.78	0.21	0.03	0.50	0.12	0.02	0.44	0.03	0.01
P值 P-value	磷源P source	0.261 3	0.953 6	0.439 4	0.510 6	0.066 2	0.074 3	0.174 4	＜0.000 1	0.042 5
	磷水平/% P level	0.698 6	0.675 5	0.876 0	0.665 8	0.818 0	0.030 3	0.019 2	＜0.000 1	＜0.000 1
	磷源×磷水平 P source×P level	0.342 0	0.296 4	0.003 6	0.526 6	0.236 0	0.253 0	0.125 7	＜0.000 1	0.912 1