表达乳铁蛋白肽的猪源罗伊氏乳酸杆菌对断乳仔猪抗霍乱沙门菌感染的效果分析

doi:10.11843/j.issn.0366-6964.2021.010.018

Abstract

Abstract: The purpose of this study was to analyze the effect of recombinant Lactobacillus reuteri from piglets expressing bovine lactoferrin peptide (pPG-LFCA-E/LR-CO21) as microecological on the growth promotion of piglets and resistance to Salmonella Choleraesuis CVCC79102 infection. In this experiment, the recombinant bacteria were continuously fed to 28-day-old weaned piglets for 21 days, and empty bacteria group, control group and antibiotic group were set up. On the 21st day, weaned piglets were orally infected with Salmonella Choleraesuis for 3 days, and the observation period was set for 7 days, and the serum, intestinal mucus and intestinal tissue of the experimental groups and the control group were collected after infection. The results showed that the average daily gain, feed gain ratio and immune organ index of piglets in the recombinant bacteria group were significantly increased (P<0.05). After being infected with Salmonella Choleraesuis CVCC79102, in the recombinant bacteria group, application of the recombinant bacteria increased the daily gain of piglets and reduced the rate of diarrhea compared with the control group; it significantly increased the amount of IgG in blood and IL-4 and sIgA in intestinal mucus (P<0.01). and significantly reduced the amount of IL-2, IL-12, IL-6 (P<0.05). It significantly increased the gene transcription level of intestinal tight junction protein ZO-1, Claudin-1 and the gene transcription levels of TLR4, Myd88 and MLCK, and significantly decreased the number of Salmonella Choleraesuis in the intestinal tract of piglets (P<0.01). There was no significant difference between the recombinant bacteria group and the antibiotic group. The above results show that pPG-LFCA-E/LR-CO21 can regulate the growth performance, intestinal morphology and intestinal barrier function of weaned piglets, and can protect piglets from Salmonella Choleraesuis infection to a certain extent, indicating that the recombinant bacteria have a certain application prospect feeding as microecological for weaned piglets.

Key words: bovine lactoferrin peptide, recombinant Lactobacillus reuteri, Salmonella Choleraesuis, weaned piglets

CLC Number:

S859.797

WANG Xueying, GAO Kang, CAI Jiyao, ZHANG Senhao, XIE Weichun, WANG Xiaona, CUI Wen, JIANG Yanping, ZHOU Han, WANG Li, QIAO Xinyuan, XU Yigang, LI Yijing, TANG Lijie. Analysis of Effects of Lactobacillus reuteri from Piglets Secreting Lactoferrin Peptides against Salmonella Choleraesuis Infection of Weaned Piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2874-2886.

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Analysis of Effects of Lactobacillus reuteri from Piglets Secreting Lactoferrin Peptides against Salmonella Choleraesuis Infection of Weaned Piglets

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