紫锥菊提取物对大鼠湿热泄泻的疗效及其机制

doi:10.11843/j.issn.0366-6964.2023.01.035

Abstract

Abstract: This study established a rat model of damp-heat diarrhea (DHD) to explore the efficacy of Echinacea purpurea extract (EPE) on DHD, so as to provide scientific basis for EPE in the prevention and treatment of DHD. Rats with DHD were established by “internal and external Dampness-heat+Escherichia coli”, and were treated with 1 and 3 g·kg^-1 doses of EPE, respectively. The DHD model was evaluated by clinical symptom score, the blood routine test, blood biochemistry, serum inflammatory factors, organ index and colon related gene expression level of rats in each group were measured, and the histopathology of rat organs was observed. The results showed that the DHD rats were fluffy, depressed, piled up, lazy, secretions appeared in the eyes, loose stool with yellow and smelly, and perianal dirt was visible. After 6 days of administration, the spirit, diet and appearance of rats returned to normal in the high and low dose EPE groups and the positive drug group. Compared with the control group, the indexes of heart, spleen and lung in the model group increased significantly (P<0.05), and the numbers of red blood cell (RBC), hemoglobin (HGB) and hematocrit (HCT) in blood decreased significantly (P<0.05); the levels of urea and alanine aminotransferase (ALT) in serum decreased significantly (P<0.05), the levels of IL-6, IL-17 and IL-23 increased significantly (P<0.05). The levels of TGF-β, IL-2 and IL-10 decreased significantly (P<0.05); the expression of colon TGF-β1 and Foxp3 mRNA decreased significantly (P<0.05), the expression of RORγt mRNA increased significantly (P<0.05); Massive bleeding and stasis in the heart, edema in the liver, edema and old bleeding in the spleen, inflammatory cell proliferation and deposition of proteinuria in the kidneys. Compared with the model group, the heart, spleen and lung of the high and low dose EPE groups were significantly lower (P<0.05), the level of HCT in blood was significantly higher (P<0.05), the levels of urea, total cholesterol (TC), total protein (TP) and albumin (ALB) in serum were significantly higher (P<0.05), the levels of IL-17 and IL-23 in serum were significantly lower (P<0.05), the level of TGF-β increased significantly (P<0.05); The expression of colon TGF-β1 and Foxp3 mRNA increased significantly (P<0.05), the expression of RORγt mRNA decreased significantly (P<0.05). The organ cell damage in high and low dose EPE groups recovered. The data may be extrapolated to suggest that Echinacea purpurea extract can alleviate DHD in rats by improving organ function, reducing blood lipid and regulating the level of serum inflammatory factors.

Key words: Echinacea purpurea extract, damp-heat diarrhea, rats

CLC Number:

S853.74

CHI Xingzi, YANG Shijing, YANG Bowen, SUN Han, YAO Lili, GU Daxing, GUO Shining, SHI Dayou, WU Li, LIU Cui. Effect and Mechanism of Echinacea purpurea Extract in Rats with Dampness-heat Diarrhea[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 380-391.

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Effect and Mechanism of Echinacea purpurea Extract in Rats with Dampness-heat Diarrhea

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