冬季不同饲养模式下哈萨克马肠道菌群多样性研究

doi:10.11843/j.issn.0366-6964.2022.10.021

Abstract

Abstract: There are few reports on the effects of different feeding modes on the intestinal flora of Kazakh horses. This study compared the composition and biomarker of the fecal flora of Kazakh horses under free grazing and captive rearing, thus to reveal the fluctuation of the intestinal flora of Kazakh horses under different feeding modes. Fresh feces were collected from 5 Kazakh horses under free grazing and 5 Kazakh horses in captivity. The composition and functional pathways of the intestinal flora of Kazakh horses were analyzed by high-throughput sequencing technology. The results showed that the diversity of intestinal flora in the grazing group was significantly higher than that in the captive group (Shannon index, P<0.05; Simpson index, P<0.01); The biomarker species of the grazing group were significantly more than that of the captive group. At the phylum level, the abundance of Firmicutes (76.10%; 78.08%) and Bacteroidetes (17.16%; 15.72%) in grazing group and captive group was higher, which accounting for more than 93% of the total flora. At the family level, cellulose decomposing/fermenting bacteria (Ruminococcaceae, 30.44%; Lachnospiraceae, 21.96%;[Mogibacteriaceae], 4.86%) were dominant in the grazing group. While the cellulose decomposition/fermentation bacteria Ruminococcaceae (28.73%) and Lachnospiraceae (14.51%), as well as the starchcarbohydrates digesting bacteria Streptococcaceae (15.08%) were dominant in the captive group. In addition, the abundance of Streptococcaceae significantly higher than that of the grazing group (P<0.05). Annotation for the functional gene sequences showed that, 77.44% of the sequences were annotated as "metabolism". The results indicated that, the colony diversity of the free grazing group was higher than that of the captive group, and the digestion type of flora was more unified. There were overall differences in the composition of the intestinal flora between the two groups, which laid a theoretical foundation for the further study of the intestinal flora of Kazakh horses.

Key words: Kazakh horse, gut microbiota, feeding mode, diversity

CLC Number:

S821.4

GAO Xueli, LI Mengmeng, Jiaerheng·BIEJIANHAN, QI·Aladaer. The Diversity of Gut Microbiota of Kazakh Horses under Different Feeding Modes in Winter[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3490-3499.

References 49

[1]	赵红艳, 孙泽威.马属动物肠道微生物的研究进展[J].经济动物学报, 2014, 18(1):59-62.ZHAO H Y, SUN Z W.Advances on microorganisms in intestine of equine[J].Journal of Economic Animal, 2014, 18(1):59-62.(in Chinese)
[2]	MAYNARD C L, ELSON C O, HATTON R D, et al.Reciprocal interactions of the intestinal microbiota and immune system[J].Nature, 2012, 489(7415):231-241.
[3]	HUANG W C, CHEN Y H, CHUANG H L, et al.Investigation of the effects of microbiota on exercise physiological adaption, performance, and energy utilization using a gnotobiotic animal model[J].Front Microbiol, 2019, 10:1906.
[4]	赵晗旭.不同野生动物肠道微生物多样性分析及功能初步研究[D].长春:吉林农业大学, 2015.ZHAO H X.Study on the diversity and function of intestinal bacterial flora from different wild animals[D].Changchun:Jilin Agricultural University, 2015.(in Chinese)
[5]	青河县人民政府.青河简介[EB/OL].[2021-06-15].http://www.xjqh.gov.cn/zjqh/003001/20181003/60ad72ab-48ec-46c1-86a4-479dbfd2ac97.html.Qinghe County People's Government.Introduction to Qinghe[EB/OL].[2021-06-15].http://www.xjqh.gov.cn/zjqh/003001/20181003/60ad72ab-48ec-46c1-86a4-479dbfd2ac97.html. (in Chinese)
[6]	喀纳斯景区管理委员会.新疆喀纳斯湖风景名胜区总体规划(2020-2035)[EB/OL].[2020-07-10].http://www.kns.gov.cn/govxxgk/001/2021-07-10/5213dbe4-fc5c-42ee-8bd1-4b1bbfb2ea4c.html.Kanas Scenic Spot Management Committee.Overall planning of Kanas lake scenic resort in Xinjiang (2020-2035)[EB/OL].[2020-07-10].http://www.kns.gov.cn/govxxgk/001/2021-07-10/5213dbe4-fc5c-42ee-8bd1-4b1bbfb2ea4c.html. (in Chinese)
[7]	丁丽媛, 杜拉提·卡衣马尔旦, ИСХАН К, 等.哈萨克马遗传资源特征研究[J].黑龙江畜牧兽医, 2018(3):91-94.DING L Y, DULATI K, ИСХАН К, et al.Study on the characteristics of genetic resources of Kazakh horse[J].Heilongjiang Animal Science and Veterinary Medicine, 2018(3):91-94.(in Chinese)
[8]	赵天佐.哈萨克马[J].新疆农业科学, 1991(5):222-223.ZHAO T Z.Kazakh horses[J].Xinjiang Agricultural Sciences, 1991(5):222-223.(in Chinese)
[9]	邢振存.蒙古马和纯血马肠道真菌多样性初步研究及分析[D].呼和浩特:内蒙古农业大学, 2016.XING Z C.Mongolian horse and thoroughbred intestinal fungal diversity preliminary research and analysis[D].Hohhot:Inner Mongolia Agricultural University, 2016.(in Chinese)
[10]	伊如格勒图.蒙古马肠道细菌多样性的研究[D].呼和浩特:内蒙古农业大学, 2015.YIRUGELETU.Analysis of intestinal bacteria diversity in Mongolian horse[D].Hohhot:Inner Mongolia Agricultural University, 2015.(in Chinese)
[11]	苏少锋.蒙古马胃肠道细菌群落组成及纤维素分解菌的研究[D].呼和浩特:内蒙古农业大学, 2019.SU S F.Characterization of gastrointestinal bacterial community and cellulose-decomposing bacteria in Mongolian horses[D].Hohhot:Inner Mongolia Agricultural University, 2019.(in Chinese)
[12]	白娜.内蒙古锡林郭勒牧区健康家畜肠道菌群多样性研究[D].呼和浩特:内蒙古农业大学, 2014.BAI N.Diversity of intestinal microbiota of healthy pastoral livestock in Inner Mongolia Xilin Gol[D].Hohhot:Inner Mongolia Agricultural University, 2014.(in Chinese)
[13]	李晓斌, 赵国栋, 刘振, 等.3~6月龄伊犁马肠道微生物群落多样性的研究[J].动物营养学报, 2017, 29(5):1535-1544.LI X B, ZHAO G D, LIU Z, et al.A study on intestinal microbiota diversity of 3- to 6-month-old Yili horses[J].Chinese Journal of Animal Nutrition, 2017, 29(5):1535-1544.(in Chinese)
[14]	李晓斌, 李海, 臧长江, 等.断奶前马驹与母马粪便菌群多样性分析[J].中国畜牧兽医, 2020, 47(4):1050-1057.LI X B, LI H, ZANG C J, et al.Diversity analysis of fecal flora on pre-weaning foal and mare[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(4):1050-1057.(in Chinese)
[15]	DE FOMBELLE A, VEIGA L, DROGOUL C, et al.Effect of diet composition and feeding pattern on the prececal digestibility of starches from diverse botanical origins measured with the mobile nylon bag technique in horses[J].J Anim Sci, 2004, 82(12):3625-3634.
[16]	HALE C, HEMMINGS A, ADJOU K.Effets de la levure vivante Actisaf Sc47 mélangée à l'aliment sur le comportement du cheval[J].La Semaine Vétérinaire, 2014, 1576.
[17]	YODER M, MILLER E, ROOK J, et al.Fiber level and form:Effects on digestibility, digesta flow and incidence of gastrointestinal disorders[C]//Proceedings of the Fifteenth Equine Nutrition & Physiology Symposium.Savoy:Equine Nutrition & Physiology Society, 1997:122-127.
[18]	DE FOMBELLE A, VARLOUD M, GOACHET A G, et al.Characterization of the microbial and biochemical profile of the different segments of the digestive tract in horses given two distinct diets[J].Anim Sci, 2003, 77(2):293-304.
[19]	DE FOMBELLE A, JULLIAND V, DROGOUL C, et al.Feeding and microbial disorders in horses:1-effects of an abrupt incorporation of two levels of barley in a hay diet on microbial profile and activities[J].J Equine Vet Sci, 2001, 21(9):439-445.
[20]	ANTWIS R E, LEA J M D, UNWIN B, et al.Gut microbiome composition is associated with spatial structuring and social interactions in semi-feral Welsh Mountain ponies[J].Microbiome, 2018, 6(1):207.
[21]	REN T T, GRIENEISEN L E, ALBERTS S C, et al.Development, diet and dynamism:Longitudinal and cross-sectional predictors of gut microbial communities in wild baboons[J].Environ Microbiol, 2016, 18(5):1312-1325.
[22]	李莉莉, 于广利.肠道微生物组:见微知著的第三次医学革命[M].北京:科学出版社, 2021.LI L L, YU G L.The gut microbiome:see the third revolution in medicine[M].Beijing:Science Press, 2021.(in Chinese)
[23]	丁丽媛.哈萨克马表型及微卫星遗传多样性研究[D].阿拉尔:塔里木大学, 2017.DING L Y.Study on the diversity of Kazakh horses phenotype and microsatellite inheritance[D].Alaer:Tarim University, 2017.(in Chinese)
[24]	周军.哈萨克马新品系(品种)培育及产品开发利用[J].山东畜牧兽医, 2020, 41(12):24-25.ZHOU J.Breeding and product development and utilization of new Kazakh horse strains(varieties)[J].Shandong Journal of Animal Science and Veterinary Medicine, 2020, 41(12):24-25.(in Chinese)
[25]	DOUGAL K, DA LA FUENTE G, HARRIS P A, et al.Identification of a core bacterial community within the large intestine of the horse[J].PLoS One, 2013, 8(10):e77660.
[26]	JULLIAND V, DE FOMBELLE A, DROGOUL C, et al.Feeding and microbial disorders in horses:Part 3-effects of three hay:Grain ratios on microbial profile and activities[J].J Equine Vet Sci, 2001, 21(11):543-546.
[27]	WILLING B, VÖRÖS A, ROOS S, et al.Changes in faecal bacteria associated with concentrate and forage-only diets fed to horses in training[J].Equine Vet J, 2009, 41(9):908-914.
[28]	SHEPHERD M L, SWECKER W S JR, JENSEN R V, et al.Characterization of the fecal bacteria communities of forage-fed horses by pyrosequencing of 16S rRNA V4 gene amplicons[J].FEMS Microbiol Lett, 2012, 326(1):62-68.
[29]	BRULC J M, ANTONOPOULOS D A, MILLER M E B, et al.Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases[J].Proc Natl Acad Sci USA, 2009, 106(6):1948-1953.
[30]	BERNINI L J, SIMÃO A N C, ALFIERI D F, et al.Beneficial effects of Bifidobacterium lactis on lipid profile and cytokines in patients with metabolic syndrome:A randomized trial.Effects of probiotics on metabolic syndrome[J].Nutrition, 2016, 32(6):716-719.
[31]	BERRY D.The emerging view of Firmicutes as key fibre degraders in the human gut[J].Environ Microbiol, 2016, 18(7):2081-2083.
[32]	SPENCE C, WELLS W G, SMITH C J, et al.Characterization of the primary starch utilization operon in the obligate anaerobe Bacteroides fragilis:regulation by carbon source and oxygen[J].J Bacteriol, 2006, 188(13):4663-4672.
[33]	WOOD T M.Cellulase of Ruminococcus albus[J].Methods Enzymol, 1988, 160:216-221.
[34]	EZAKI T.Ruminococcus[M].New York:John Wiley & Sons Ltd., 2015.
[35]	PATRA A K, YU Z T.Essential oils affect populations of some rumen bacteria in vitro as revealed by microarray (RumenBactArray) analysis[J].Front Microbiol, 2015, 6:297.
[36]	ZHAO X H, CHEN Z D, ZHOU S, et al.Effects of daidzein on performance, serum metabolites, nutrient digestibility, and fecal bacterial community in bull calves[J].Anim Feed Sci Technol, 2017, 225:87-96.
[37]	SHINKAI T, UEKI T, KOBAYASHI Y.Detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes[J].Anim Sci J, 2010, 81(1):72-79.
[38]	杨明, 袁悦, 李宪臻, 等.不同环境中纤维素降解菌群多样性差异分析[J].江西农业大学学报, 2020, 42(1):174-186.YANG M, YUAN Y, LI X Z, et al.Differential analysis of diversity in cellulose-decomposing bacterial communities from diverse environments[J].Acta Agriculturae Universitatis Jiangxiensis, 2020, 42(1):174-186.(in Chinese)
[39]	PEDERSEN H K, GUDMUNDSDOTTIR V, NIELSEN H B, et al.Human gut microbes impact host serum metabolome and insulin sensitivity[J].Nature, 2016, 535(7612):376-381.
[40]	SUN B H, WANG X, BERNSTEIN S, et al.Marked variation between winter and spring gut microbiota in free-ranging Tibetan Macaques (Macaca thibetana)[J].Sci Rep, 2016, 6:26035.
[41]	李倩, 黄新新, 张文杰, 等.不同谷物淀粉对断奶马驹盲肠菌群多样性的影响[J].动物营养学报, 2022, 34(1):488-499.LI Q, HUANG X X, ZHANG W J, et al.Effects of cereal starches on cecal microflora diversity of weaned foals.[J].Chinese Journal of Animal Nutrition, 2022, 34(1):488-499.(in Chinese)
[42]	VARLOUD M, FONTY G, ROUSSEL A, et al.Postprandial kinetics of some biotic and abiotic characteristics of the gastric ecosystem of horses fed a pelleted concentrate meal[J].J Anim Sci, 2007, 85(10):2508-2516.
[43]	BANIEL A, AMATO K R, BEEHNER J C, et al.Seasonal shifts in the gut microbiome indicate plastic responses to diet in wild geladas[J].Microbiome, 2021, 9(1):26.
[44]	ARNOLD F F.Prececal, postileal and total tract starch digestion in ponies fed corn, oats, barley or sorghum grain[D].Texas:Texas A&M University, 1982.
[45]	MEDINA B, GIRARD I D, JACOTOT E, et al.Effect of a preparation of Saccharomyces cerevisiae on microbial profiles and fermentation patterns in the large intestine of horses fed a high fiber or a high starch diet[J].J Anim Sci, 2002, 80(10):2600-2609.
[46]	HARLOW B E, LAWRENCE L M, FLYTHE M D.Diarrhea-associated pathogens, Lactobacilli and cellulolytic bacteria in equine feces:Responses to antibiotic challenge[J].Vet Microbiol, 2013, 166(1-2):225-232.
[47]	赵芳芳, 王洪荣, 张营, 等.谷物作为反刍动物能量饲料的营养特性及高谷物饲粮对消化道健康的影响[J].动物营养学报, 2020, 32(6):2476-2482.ZHAO F F, WANG H R, ZHANG Y, et al.Nutritional characteristics of grain as energy feed for ruminants and effects of high-grain diet on gastrointestinal tract health[J].Chinese Journal of Animal Nutrition, 2020, 32(6):2476-2482.(in Chinese)
[48]	KAYA G, SOMMERFELD-STUR I, IBEN C, et al.Risk factors of colic in horses in Austria[J].J Anim Physiol Anim Nutr, 2009, 93(3):339-349.
[49]	GONÇALVES S, JULLIAND V, LEBLOND A.Risk factors associated with colic in horses[J].Vet Res, 2002, 33(6):641-652.

The Diversity of Gut Microbiota of Kazakh Horses under Different Feeding Modes in Winter

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