基于靶向代谢组学研究环形泰勒虫对宿主细胞能量代谢的影响

doi:10.11843/j.issn.0366-6964.2021.012.020

Abstract

Abstract: The Theileria annulata infected host cells obtained unlimited proliferation as tumor cells, and the phenomenon of parasite-dependent transformation is reversible. The T. annulata transformed cells provide an ideal model for better understanding the molecular mechanism of parasite-host cells interactions. In the present study, the targeted metabolomics method was applied out to investigate the changes of metabolites of the transformed cells related with energy metabolism. Aimed to investigate the difference of cells proliferation, Buparvaquone treatment group (BW720c), DMSO control group and blank control group were set. Cell samples were collected at 72 h, and the energy metabolism-related metabolites of the cell samples were detected by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) targeted metabolomics method. Then, the compound types of the metabolites were identified by the energy database. The differential metabolites were screened by the Partial Least Squares-Discriminant Analysis (PLS-DA).With the results of cells number counting, it was found that there has no significant change between the three groups within 24 h. However, the cell growth rate of the experimental group was significantly lower than the other two control groups at 36 h, and the difference between the two groups was largest at 72 h. PLS-DA analysis showed that metabolites variables could be clustered and distinguished between the drug treatment group and DMSO control, and 21 differential metabolites between two groups were screened out. In the drug treatment group, 8 metabolites were up-regulated and 13 metabolites were down-regulated. After enrichment analysis, it was found that these metabolites were mainly related to energy metabolism, such as glutamine, L-lactic, malic, pyruvic acid, and isocitrate. Meanwhile, it was found that the changes of glutamine, L-actic and pyruvic acid contents in the drug treatment group and the control group were consitent with the results of targeted metabolomics detection by using the corresponding kits. The results indicated that T. annulata infection can significantly affect the energy metabolism of the host cells, which lays a foundation to illustrate the mechanisms of cells transformation by T. annulata.

Key words: Theileria annulata transformed cells, targeted metabolomics, energy metabolism

CLC Number:

S855.9

LI Xia, LI Zhi, CAO Tianxing, YIN Hong, LUO Jianxun, GUAN Guiquan, LIU Junlong, ZHAO Hongxi. Effects of Theileria annulata Infection on Energy Metabolism of Host Cells Based on Targeted Metabolomics[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3535-3545.

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Effects of Theileria annulata Infection on Energy Metabolism of Host Cells Based on Targeted Metabolomics

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