葛根素对饲喂氧化大豆油肉鸡小肠黏膜屏障功能及抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2021.012.018

Abstract

Abstract: The experiment was conducted to investigate the effects of puerarin on mucosal morphology, mRNA expression of tight junction protein and antioxidant capacity of small intestine in yellow-feathered broilers fed oxidized soybean oil. The experiment was designed with 2×2 factors including oil quality (fresh soybean oil and oxidized soybean oil) and puerarin addition levels (0, 750 mg·kg^-1). A total of 360 1-day-old healthy female yellow-feathered broilers were randomly divided into 4 treatment groups with 6 replicates per group and 15 chickens per replicate, which were fresh soybean oil diet group, fresh soybean oil + puerarin (750 mg·kg^-1) diet group, oxidized soybean oil diet group and oxidized soybean oil + puerarin (750 mg·kg^-1) diet group, respectively. At 28 and 56 days of age, one chicken per replicate was randomly selected, and the morphology of small intestinal mucosa, the mRNA expression of tight junction protein and antioxidant indices of small intestine mucosa were determined. The results showed that:1) Consumption of oxidized soybean oil significantly decreased villus height and villus height/crypt depth (V/C) in duodenum of 28-day-old broilers, V/C and mRNA expression levels of claudin-1 in ileum, villus height and V/C in three intestinal segments of 56-day-old broilers (P<0.05). Consumption of oxidized soybean oil significantly increased crypt depth of three intestinal segments at 56 days of age (P<0.05). Dietary supplementation of puerarin significantly increased villus height and V/C in jejunum and Zonula occluden1 (ZO-1) mRNA expression level in ileum of broilers at 28 days of age, and V/C in duodenum, jejunum and ileum and villus height in jejunum and ileum of broilers at 56 days of age (P<0.05), and significantly decreased crypt depth in three intestinal segments (P<0.05). 2) Oxidized soybean oil significantly increased the glutathione (GSH) content in duodenum and activity of glutathion peroxidase (GSH-Px)in jejunum at 28 days of age (P<0.05), and significantly decreased the GSH content, superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) in ileum at 28 days of age (P<0.05). Dietary supplementation of puerarin significantly increased the activity of GSH-Px in duodenum of broilers at 28 days of age (P<0.05). Dietary oxidized soybean oil significantly decreased the activity of SOD and T-AOC in ileum at 56 days of age (P<0.05), dietary supplementation of puerarin significantly increased the activities of SOD in duodenum and ileum, and GSH-Px in ileum (P<0.05), and significantly decreased the content of GSH and the activity of SOD, T-AOC in jejunum and T-AOC in ileum (P<0.05) of broilers at 56 days of age. In conclusion, consumption of oxidized soybean oil damaged intestinal mucosal morphology and decreased the mRNA expression of tight junction protein and antioxidant capacity of small intestinal. Puerarin could increase the mRNA expression of tight junction protein in small intestinal mucosa of broilers, improve the morphological structure of intestinal mucosa under oxidative injury, increase the activity of antioxidant enzymes and enhance the antioxidant capacity of broilers.

Key words: puerarin, oxidized soybean oil, broilers, mucosal morphology, antioxidant capacity, tight junction protein

CLC Number:

S831.5

YU Yingmei, SHI Xuan, OUYANG Jingxin, LIU Sanfeng, LI Guanhong. Effects of Puerarin on Intestinal Barrier Function and Antioxidant Capacity of Broilers Fed with Oxidized Soybean Oil[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3512-3523.

References 26

[1]	QAISRANI S N, RIZWAN M, YASEEN G, et al.Effects of dietary oxidized oil on growth performance, meat quality and biochemical indices in poultry-a review[J].Ann Anim Sci, 2021, 21(1):29-46.
[2]	STAPRÃNS I, RAPP J H, PAN X M, et al.Oxidized lipids in the diet are a source of oxidized lipid in chylomicrons of human serum[J].Arterioscler Thromb, 1994, 14(12):1900-1905.
[3]	HAYAM I, COGAN U, MOKADY S.Dietary oxidized oil and the activity of antioxidant enzymes and lipoprotein peroxidation in rats[J].Nutr Res, 1995, 15(7):1037-1044.
[4]	KELLER U, BRANDSCH C, EDER K.Supplementation of vitamins C and E increases the vitamin E status but does not prevent the formation of oxysterols in the liver of guinea pigs fed an oxidised fat[J].Eur J Nutr, 2004, 43(6):353-359.
[5]	LAMBERT G P.Stress-induced gastrointestinal barrier dysfunction and its inflammatory effects[J].J Anim Sci, 2009, 87(S 14):E101-E108.
[6]	HE S P, AROWOLO M A, MEDRANO R F, et al.Impact of heat stress and nutritional interventions on poultry production[J].World Poult Sci J, 2018, 74(4):647-664.
[7]	LINDBLOM S C, GABLER N K, BOBECK E A, et al.Oil source and peroxidation status interactively affect growth performance and oxidative status in broilers from 4 to 25 d of age[J].Poult Sci, 2019, 98(4):1749-1761.
[8]	LAIKA M, JAHANIAN R.Dietary Supplementation of organic selenium could improve performance, antibody response, and yolk oxidative stability in laying hens fed on diets containing oxidized fat[J].Biol Trace Elem Res, 2015, 165(2):195-205.
[9]	LIU L L, HE J H, XIE H B, et al.Resveratrol induces antioxidant and heat shock protein mRNA expression in response to heat stress in black-boned chickens[J].Poult Sci, 2014, 93(1):54-62.
[10]	BEBREVSKA L, FOUBERT K, HERMANS N, et al.In vivo antioxidative activity of a quantified Pueraria lobata root extract[J].J Ethnopharmacol, 2010, 127(1):112-117.
[11]	YUAN D X, HUSSAIN T, TAN B, et al.The evaluation of antioxidant and anti-inflammatory effects of Eucommia ulmoides flavones using diquat-challenged piglet models[J].Oxid Med Cell Longev, 2017, 2017:8140962.
[12]	RIBEIRO D, FREITAS M, LIMA J L F C, et al.Proinflammatory pathways:The modulation by flavonoids[J].Med Res Rev, 2015, 35(5):877-936.
[13]	孙英新, 张娟, 闫永建, 等.葛根素对百草枯诱导大鼠肺纤维化转化生长因子β1的影响[J].中国中医药咨讯, 2010, 2(29):32-33, 117.SUN Y X, ZHANG J, YAN Y J, et al.Effect of puerarin on transforming growth factor β1of paraquat-induced pulmonary fibrosis in rats The impact of 1[J].Journal of China Traditional Chinese Medicine Information, 2010, 2(29):32-33, 117.(in Chinese)
[14]	梁俊清, 丁春花, 凌亦凌, 等.葛根素对糖尿病致肺损伤的保护作用及其机制的研究[J].中国应用生理学杂志, 2007, 23(3):355-359.LIANG J Q, DING C H, LING Y L, et al.The protective function of puerarin to the injury of the lung and it's mechanisms during diabetes[J].Chinese Journal of Applied Physiology, 2007, 23(3):355-358.(in Chinese)
[15]	LIANG F F, JIANG S Q, MO Y, et al.Consumption of oxidized soybean oil increased intestinal oxidative stress and affected intestinal immune variables in yellow-feathered broilers[J].Asian-Australas J Anim Sci, 2015, 28(8):1194-1201.
[16]	李秋粉.益生菌对热应激肉鸡肠道屏障功能的影响[D].南昌:江西农业大学, 2017.LI Q F.Effect of probiotic on intestinal barrier function of heat-stressed broilers[D].Nanchang:Jiangxi Agricultural University, 2017.(in Chinese)
[17]	MAGNOTTI L J, DEITCH E A.Burns, bacterial translocation, gut barrier function, and failure[J].J Burn Care Rehabil, 2005, 26(5):383-391.
[18]	张志浩.氧化应激对肉鸡肠道粘膜屏障功能的影响以及缓解肠道氧化损伤物质的研究[D].泰安:山东农业大学, 2014.ZHANG Z H.Effects of oxidative Stress on the intestinal barrier function of broiler chickens and the research of substance to relieve the injury[D].Tai'an:Shandong Agricultural University, 2014.(in Chinese)
[19]	ZHANG L, DU S Y, LU Y, et al.Influence of puerarin, paeoniflorin, and menthol on structure and barrier function of tight junctions in MDCK and MDCK-MDR1 Cells[J].J Traditi Chin Med Sci, 2015, 2(2):111-119.
[20]	WANG X W, FAN F G, CAO Q.Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption[J].Mol Med Rep, 2016, 14(2):1173-1179.
[21]	JEON Y D, LEE J H, LEE Y M, et al.Puerarin inhibits inflammation and oxidative stress in dextran sulfate sodium-induced colitis mice model[J].Biomed Pharmacother, 2020, 124:109847.
[22]	TIAN T, WANG Z L, ZHANG J H.Pathomechanisms of oxidative stress in inflammatory bowel disease and potential antioxidant therapies[J].Oxid Med Cell Longev, 2017, 2017:4535194.
[23]	LINDBLOM S C, GABLER N K, BOBECK E A, et al.Oil source and peroxidation status interactively affect growth performance and oxidative status in broilers from 4 to 25 d of age[J].Poult Sci, 2019, 98(4):1749-1761.
[24]	刘忠臣, 陈代文, 余冰, 等.不同脂肪来源对断奶仔猪生长性能和脂类代谢的影响[J].动物营养学报, 2011, 23(9):1466-1474.LIU Z C, CHEN D W, YU B, et al.Effects of different fat sources on growth performance and lipid metabolism of weaned piglets[J].Chinese Journal of Animal Nutrition, 2011, 23(9):1466-1474.(in Chinese)
[25]	LI X Y, YANG Z, GREENWOOD M T.Gα protein dependent and independent effects of human RGS1 expression in yeast[J].Cell Signal, 2004, 16(1):43-49.
[26]	WU Y Y, XUE B, LI X J, et al.Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress[J].Neural Regen Res, 2012, 7(33):2583-2591.

Effects of Puerarin on Intestinal Barrier Function and Antioxidant Capacity of Broilers Fed with Oxidized Soybean Oil

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 26

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Hong, RUAN Hongri, MA Tianwen, LI Yanan, MIAO Xue, YANG Wenyue, GAO Li, WEI Chengwei. The Mechanism of Puerarin Improving Cartilage Degeneration in PTOA Rats by Interfering with Oxidative Stress and Nrf2/HO-1 Pathway of Cartilage [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3951-3963.
[2]	LIU Yankun, LUO Runbo, LIN Yan, ZHU Weiyun. Effects of Phage Cocktail on Growth Performance, Blood Parameters and Fecal Microbiota of Weaned Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1555-1567.
[3]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[4]	QIN Shizhen, YANG Minmin, REN Zhixiong, LI Jinlu, TANG Defu, SHI Zhaoguo. Effects of Bacillus subtilis on Intestinal Immunity, Intestinal Tissue Morphology and Intestinal Barrier of Broilers Challenged with Lipopolysaccharide [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4676-4690.
[5]	WEI Zhangyi, HAN Fei, FAN Tingting, CHEN Zixian, LI Hanmei, YUAN Feifan, CONG Rihua, LI Xian. Regulation of Kushen-cangzhu Granules on Hepatic Cholesterol Metabolism in Broilers Infected with Escherichia coli [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 392-402.
[6]	DING Jiqiang, LI Qinghe, ZHANG Gaomeng, LI Sen, ZHENG Maiqing, WEN Jie, ZHAO Guiping. Comparing the Accuracy of Estimated Breeding Value by Several Algorithms on Laying Traits in Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1364-1372.
[7]	ZHANG Lihuan, JIA Hao, WANG Yanfei, ZHANG Ruonan, LIU Xuan. Effects of Healthy Tribeneficial Bacteria on Lipid Metabolism in Broilers Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1154-1164.
[8]	ZHANG Xueqing, TONG Xishuai, WANG Guoshuai, BIAN Jianchun, LIU Xuezhong, YUAN yan, ZOU Hui, SHEN Xiaolan, LIU Zongping, GU Jianhong. Puerarin Alleviates the Inhibitory Effect of Cadmium on the Femoral and Tibial Osteoblast Differentiation of Rat [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 628-636.
[9]	WANG Ruixiu, CHEN Zhongwei, LIU Qiang, ZHUANG Su. Effects of Synbiotics on Meat Quality, Antioxidant Capacity and Immune Function of Cherry Valley Ducks [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2522-2533.
[10]	LIU Yingsheng, JIAO Hongchao, LIN Hai, WANG Xiaojuan. Effect of Early Heat Stress on the Growth and Development of Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2052-2058.
[11]	WU Zhengke, ZHANG Anrong, CHEN Zhimin, CHANG Wenhuan, LIU Guohua, CAI Huiyi, ZHENG Aijuan. Effects of Bacillus Coagulans on Growth Performance, Serum Biochemical Indices and Tibia Strength Indices of Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1031-1041.
[12]	LIU Ming, ZHANG Lei, AN Xiaopeng, ZHANG Enping, SONG Yuxuan. Effects of Different Ammonia Concentrations on Growth Performance, Immunity and Antioxidant Capacity of Beef Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2757-2764.
[13]	PENG Xiaokun, ZHAO Tian, HUANG Xiaoyu, ZHANG Yu, XING Xiaonan, ZHANG Enping. Effects of Acute Heat Stress on Blood Biochemistry Indices and Expression of HSP70 Family Genes in Blood Lymphocytes in Goats [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1219-1229.
[14]	GAN Shiyi, WANG Jie, LUO Chenglong, QU Hao, SHU Dingming. Association of Genetic Resistance Loci to Subgroup A Avian Leukosis Virus with Important Economic Traits in Broilers [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 721-726.
[15]	FAN Yi-xuan, DENG Kai-ping, TANTAI Wen-jing, HUANG Xin-ai, WANG Feng, CHEN Ming. Effect of Selenium Yeast in Containing PUFAs Dietary on Fatty Acid Composition and Antioxidant Capacity of Hu Lambs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1661-1673.