1株分离自云南省师宗县库蠓的新型西藏环状病毒

doi:10.11843/j.issn.0366-6964.2021.017

Abstract

Abstract: Tibet orbivirus (TIBOV) is a new member of the genus Orbivirus. The virus was first isolated from Anopheles maculatus collected in Motuo County, Tibet, China in 2009. At present, the recognition of this virus is still very limited. This is the first report on the isolation and identification of a new TIBOV strain YNSZ/V290/2019 from Culicoides collected in Shizong County, Yunnan Province, China. YNSZ/V290/2019 can cause an obvious cytopathic effect on C6/36 and BHK-21 cells. Under the electron microscope, the virus particles are spherical, with a diameter of about 55-75 nm, and have typical morphological characteristics of cycloviruses. The results of agarose gel electrophoresis showed that the genome of the virus was a 10 segment double-stranded RNA, showing the characteristics of "3-3-3-1". The sequence analysis of the highly conserved gene (Seg-3) and proteins (T2) showed that YNSZ/V290/2019 was a member of TIBOV, and YNSZ/V290/2019 shared 80.90%-81.70% nucleic acid sequence identity and 97.20%-97.30% amino acid sequence identity with other TIBOV strains. The sequence analysis of the high variable gene (Seg-2) and proteins (OC1) which determine the serotype of the Orbivirus showed that YNSZ/V290/2019 shared 26.90%-30.60% nucleic acid sequence identity and 28.5%-33.2% amino acid sequence identity with the known TIBOV strains, suggested that the strain may represent a new serotype of TIBOV. Serum neutralization tests were carried out on 40 cattle and 68 goat serum samples collected from Shizong pasture. The results showed that the positive rates of new TIBOV of cattle and sheep were 22.50% and 4.41%, respectively. The discovery of a new type of TIOBV in China enriches the research on arbovirus in China and provides a basis for further study on the distribution, pathogenicity, and host range of infection and development of diagnostic reagents of TIBOV in China.

Key words: Culicoides, virus isolation and identification, Tibet orbivirus (TIBOV), sequence analysis

CLC Number:

S852.65

YANG Zhenxing, XIE Jiarui, LI Zhanhong, LI Zhuoran, LIAO Defang, NIU Baosheng, YAO Pingfen, LI Le, YANG Heng. Isolation and Identification of a Novel Type Tibet Orbivirus Strain from Culicoides Collected in Shizong County of Yunnan Province[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 166-176.

References 33

[1]	FAUQUET C M, MAYO M A, MANILOFF J, et al. Virus taxonomy:eighth report of the International Committee on the taxonomy of viruses[M]. San Diego, CA, USA:Elsevier, 2005:988-992.
[2]	ATTOUI H, MAAN S, ANTHONY S J, et al. Bluetongue virus, other orbiviruses and other reoviruses:their relationships and taxonomy[M]//MELLOR P S, BAYLIS M, MERTENS P P C. Bluetongue. London:Academic Press, 2009:23-52.
[3]	MACLACHLAN N J. Bluetongue:history, global epidemiology, and pathogenesis[J]. Prev Vet Med, 2011, 102(2):107-111.
[4]	CARPENTER S, MELLOR P S, FALL A G, et al. African horse sickness virus:history, transmission, and current status[J]. Annu Rev Entomol, 2017, 62:343-358.
[5]	SAVINI G, AFONSO A, MELLOR P, et al. Epizootic haemorragic disease[J]. Res Vet Sci, 2011, 91(1):1-17.
[6]	CARPENTER S, WILSON A, MELLOR P S. Culicoides and the emergence of bluetongue virus in northern Europe[J]. Trends Microbiol, 2009, 17(4):172-178.
[7]	WILSON W C, RUDER M G, KLEMENT E, et al. Genetic characterization of epizootic hemorrhagic disease virus strains isolated from cattle in Israel[J]. J Gen Virol, 2015, 96(6):1400-1410.
[8]	TEMIZEL E M, YESILBAG K, BATTEN C, et al. Epizootic hemorrhagic disease in cattle, western Turkey[J]. Emerg Infect Dis, 2009, 15(2):317-319.
[9]	杨振兴, 孟锦昕, 肖雷, 等. 流行性出血病病毒(EHDV)血清7型毒株在中国的首次分离与鉴定[J]. 畜牧兽医学报, 2019, 50(3):602-610.YANG Z X, MENG J X, XIAO L, et al. Isolation of epizootic haemorrhagic disease virus serotype 7 strain in China for the first time[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(3):602-610. (in Chinese)
[10]	ROY P. Bluetongue virus structure and assembly[J]. Curr Opin Virol, 2017, 24:115-123.
[11]	BELAGANAHALLI M N, MAAN S, MAAN N S, et al. Full genome sequencing and genetic characterization of eubenangee viruses identify pata virus as a distinct species within the Genus orbivirus[J]. PLoS One, 2012, 7(3):e31911.
[12]	MAAN S, MAAN N S, SAMUEL A R, et al. Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes[J]. J Gen Virol, 2007, 88(2):621-630.
[13]	BUMBAROV V, GOLENDER N, JENCKEL M, et al. Characterization of bluetongue virus serotype 28[J]. Transbound Emerg Dis, 2020, 67(1):171-182.
[14]	SHIRAFUJI H, KATO T, YAMAKAWA M, et al. Characterization of genome segments 2, 3 and 6 of epizootic hemorrhagic disease virus strains isolated in Japan in 1985-2013:identification of their serotypes and geographical genetic types[J]. Infect, Genet Evol, 2017, 53:38-46.
[15]	WELLBY M P, BAYLIS M, RAWLINGS P, et al. Effect of temperature on survival and rate of virogenesis of African horse sickness virus in Culicoides variipennis sonorensis (Diptera:Ceratopogonidae) and its significance in relation to the epidemiology of the disease[J]. Bull Entomol Res, 1996, 86(6):715-720.
[16]	KING A M Q, ADAMS M J. Virus Taxonomy:Ninth Report of the International Committee on Taxonomy of Viruses[M]. San Diego, CA, USA:Elsevier, 2012:1221-1234
[17]	FAGRE A C, LEE J S, KITYO R M, et al. Discovery and characterization of bukakata orbivirus (Reoviridae:Orbivirus), a novel virus from a ugandan bat[J]. Viruses, 2019, 11(3):209.
[18]	COOPER E, ANBALAGAN S, KLUMPER P, et al. Mobuck virus genome sequence and phylogenetic analysis:identification of a novel Orbivirus isolated from a white-tailed deer in Missouri, USA[J]. J Gen Virol, 2014, 95(1):110-116.
[19]	TANGUDU C S, CHARLES J, HURT S L, et al. Skunk River virus, a novel orbivirus isolated from Aedes trivittatus in the United States[J]. J Gen Virol, 2019, 100(2):295-300.
[20]	ATTOUI H, JAAFAR F M, BELHOUCHET M, et al. Yunnan orbivirus, a new orbivirus species isolated from Culex tritaeniorhynchus mosquitoes in China[J]. J Gen Virol, 2005, 86(12):3409-3417.
[21]	杨恒, 李占鸿, 张怡轩, 等. 牛血液中一株新型环状病毒的分离与全基因组序列分析[J]. 病毒学报, 2018, 34(1):75-84.YANG H, LI Z H, ZHANG Y X, et al. Isolation and genomic characterization of a novel orbivirus strain from the blood of cattle[J]. Chinese Journal of Virology, 2018, 34(1):75-84. (in Chinese)
[22]	LI M H, ZHENG Y Y, ZHAO G Y, et al. Tibet Orbivirus, a novel Orbivirus species isolated from Anopheles maculatus mosquitoes in Tibet, China[J]. PLoS One, 2014, 9(2):e88738.
[23]	范娜, 曹玉玺, 付士红, 等. 三带喙库蚊中分离的西藏环状病毒全基因序列测定及分析[J]. 病毒学报, 2018, 34(5):625-637.FAN N, CAO Y X, FU S H, et al. Genome sequencing and analyses of the Tibet orbivirus isolated from Culex tritaeniorhynchus[J]. Chinese Journal of Virology, 2018, 34(5):625-637. (in Chinese)
[24]	WANG J L, LI H C, HE Y W, et al. Isolation of Tibet orbivirus from Culicoides and associated infections in livestock in Yunnan, China[J]. Virol J, 2017, 14(1):105.
[25]	LEI W W, GUO X F, FU S H, et al. Isolation of Tibet orbivirus, TIBOV, from Culicoides collected in Yunnan, China[J]. PLoS One, 2015, 10(8):e0136257.
[26]	ATTOUI H, STIRLING J M, MUNDERLOH U G, et al. Complete sequence characterization of the genome of the St Croix River virus, a new orbivirus isolated from cells of Ixodes scapularis[J]. J Gen Virol, 2001, 82(4):795-804.
[27]	MAAN S, RAO S J, MAAN N S, et al. Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses[J]. J Virol Methods, 2007, 143(2):132-139.
[28]	KATOH K, ASIMENOS G, TOH H. Multiple alignment of DNA sequences with MAFFT[M]//POSADA D. Bioinformatics for DNA Sequence Analysis. New York:Humana Press, 2009:39-64.
[29]	TAMURA K, STECHER G, PETERSON D, et al. MEGA6:molecular evolutionary genetics analysis version 6. 0[J]. Mol Biol Evol, 2013, 30(12):2725-2729.
[30]	张念祖, 张开礼, 李志华, 等. 绵羊蓝舌病的调查研究报告[J]. 云南畜牧兽医,1989(4):3-13.ZHANG N Z, ZHANG K L, LI Z H, et al. Investigation report on bluetongue disease of sheep[J]. Yunnan Animal Husbandry and Veterinary Medicine, 1989(4):3-13.
[31]	YANG H, XIAO L, WANG J, et al. Phylogenetic characterization genome segment 2 of bluetongue virus strains belonging to serotypes 5, 7 and 24 isolated for the first time in China during 2012 to 2014[J]. Transbound Emerg Dis, 2017, 64(4):1317-1321.
[32]	杨恒, 肖雷, 李占鸿, 等. 2012-2016年中国南方地区帕利亚姆血清群病毒的分离与序列特征分析[J]. 畜牧兽医学报, 2018, 49(4):761-770.YANG H, XIAO L, LI Z H, et al. Sequence analysis of Palyam serogroup virus isolated in South China from 2012 to 2016[J]. Acta Veterinaria et Zootechnica Sinica, 2018, 49(4):761-770. (in Chinese)
[33]	孟锦昕, 李楠, 肖雷, 等. 云南黄牛血液中阿卡斑病毒的分离鉴定及S基因序列分析[J]. 中国兽医学报, 2019, 39(9):1703-1709.MENG J X, LI N, XIAO L, et al. Sequence analysis of S gene of two strain of Akabane virus isolated from Yunnan Province[J]. Chinese Journal of Veterinary Science, 2019, 39(9):1703-1709. (in Chinese)

Isolation and Identification of a Novel Type Tibet Orbivirus Strain from Culicoides Collected in Shizong County of Yunnan Province

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