基于RNA-Seq筛选ALV-J感染汶上芦花鸡肝差异表达致病相关基因

doi:10.11843/j.issn.0366-6964.2020.08.025

Abstract

Abstract: The aim of this study was to screen the differentially expressed pathogenic genes in liver of Wenshang Barred chicken infected by subgroup J avian leukosis virus(ALV-J). ALV uninfected and infected Wenshang Barred chickens were pre-screened by virus isolation from blood at 42 and 300 days of age. The liver tissue samples from both ALV infected chickens which had the pathological changes and ALV uninfected chickens were identified accurately by PCR detection,then the liver tissues from 3 ALV-positive (G1 group)individuals and 3 ALV-negative (G2 group) individuals were sampled for further study. PCR ampilification products of 3 ALV-positive individuals were confirmed as subgroup J by sequencing and cluster analysis. The liver tissue samples from both 3 ALV-J positive individuals and 3 ALV-negative individuals were selected for screening the differentially expressed genes by RNA-Seq technology,then functional analysis was conducted. The partial differentially expressed genes were verified by qRT-PCR. The results showed that 42 differentially expressed genes were enriched in GO and KEGG databases, which included 18 up-regulated genes and 24 down-regulated genes. Moreover, expressions of 5 differentially expressed genes randomly selected were verified by qRT-PCR, which confirmed that mRNA expression levels were consistent with RNA-Seq sequencing results. GO analysis results indicated that the differentially expressed genes were mainly involved in cellular processes, metabolic processes,response to stimulus, immune system, and so on. KEGG analysis results showed that the differentially expressed genes were mainly enriched in cellular processes, signal transduction, disease, metabolism pathways. This study found many genes and critical signaling pathways which affected the pathogenicity of ALV-J by transcriptome analysis and would provide new ideas for understanding the pathogenic mechanism of ALV-J at molecular level.

Key words: Wenshang Barred chicken, ALV-J, RNA-Seq, differentially expressed gene

CLC Number:

S831.2

ZHANG Huiyong, WU Jingsheng, YANG Jianbo, YU Yan, XUE Qian, YIN Jianmei, ZHU Yunfen, ZHU Jing, SU Yijun, LI Guohui, HAN Wei. Application of RNA-Seq Technology for Screening the Differentially Expressed Pathogenic Genes in Wenshang Barred Chicken Liver Infected by Subgroup J Avian Leukosis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 2003-2011.

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Application of RNA-Seq Technology for Screening the Differentially Expressed Pathogenic Genes in Wenshang Barred Chicken Liver Infected by Subgroup J Avian Leukosis Virus

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