体外成熟液中添加PAF对牦牛卵母细胞发育能力及基因表达的影响

doi:10.11843/j.issn.0366-6964.2020.03.011

Abstract

Abstract: The objectives of this study were to evaluate the effects of platelet-activating factor (PAF) supplementation in in vitro maturation (IVM) medium on yak (Bos grunniens) oocyte development competence and gene expression. A total of 1 025 yak cumulus-oocyte complexes (COCs) were assigned to 4 groups. COCs in different groups were matured in IVM medium with different concentrations of PAF (0, 10^-8, 10^-7, and 10^-6mol·L^-1), followed by in vitro fertilization (IVF) with cattle sperm and in vitro culture (IVC). The maturation rate, cleavage rate and blastocyst rate of oocytes, and the relative expression level of pro-apoptosis (BAX), anti-apoptosis (BCL-2), epidermal growth factor (EGF) and its receptor (EGFR), transcription factors (OCT-4 and NANOG) and c-fos genes in COCs and embryos were compared among different groups. The results showed that oocytes in IVM medium with 10^-7mol·L^-1 PAF had higher maturation rate((92.16±0.19)%), cleavage rate((77.20±0.85)%) and blastocyst rate((46.71±0.68)%) than those in the other groups (P<0.05). The results of qPCR showed that the expression level of BCL-2, EGF, EGFR, c-fos were significantly up-regulated (P<0.05), but the expression level of pro-apoptotic gene (BAX) was significantly down-regulated in matured COCs (P<0.05) in 10^-7 mol·L^-1 PAF group. The expression level of EGF, EGFR and OCT-4 in blastocysts were significantly up-regulated in 10^-7 mol·L^-1 PAF group (P<0.05). The expression level of BCL-2, BAX and NANOG genes in 2-, 4-, 8-cell, morula and blastocyst stages were not different among different groups, but the expression of BCL-2 was down-regulated and BAX was up-regulated in morula and blastocyst. In conclusion, the optimal PAF concentration in IVM medium (10^-7mol·L^-1) could improve oocyte maturation and embryo development, and regulate the expression of apoptosis and proliferation related genes of the yak.

Key words: yak, platelet-activating factor, oocyte, in vitro fertilization, qPCR

CLC Number:

S823.85

WANG Qin, XIONG Yan, ZI Xiangdong. Effects of PAF Supplementation during in vitro Maturation Medium on Yak Oocyte Development Competence and Gene Expression[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 514-523.

References 40

[1]	罗晓林.中国牦牛[M].成都:四川科学技术出版社,2019:105-154.LUO X L. China yak[M]. Chengdu:Sichuan Science and Technology House, 2019:105-154.(in Chinese)
[2]	YU S J,JU X H,WANG L B,et al.Successful embryo transfer in Tianzhu white yak using standard protocol[J].Sci China Ser C Life Sci,2007,50(5):655-659.
[3]	ZI X D,YIN R H,CHEN S W,et al.Developmental competence of embryos derived from reciprocal in vitro fertilization between yak (Bos grunniens) and cattle (Bos taurus)[J].J Reprod Dev,2009,55(5):480-483.
[4]	孙永刚,徐惊涛,才让东智,等.体外受精生产犏牛胚胎与移植试验研究[J].畜牧兽医学报,2013,44(5):719-726.SUN Y G,XU J T,CAIRANG D Z,et al.The study on cattle×yak in vitro fertilization and embryo transfer[J].Acta Veterinaria et Zootechnica Sinica,2013,44(5):719-726.(in Chinese)
[5]	XIAO X,ZI X D,NIU H R,et al.Effect of addition of FSH,LH and proteasome inhibitor MG132 to in vitro maturation medium on the developmental competence of yak (Bos grunniens) oocytes[J].Reprod Biol Endocrinol,2014,12:30.
[6]	郑杰,蒲思颖,杨远潇,等.基于高通量测序的犏牛囊胚玻璃化冷冻损伤机制研究[J].畜牧兽医学报,2017,48(10):1871-1881.ZHENG J,PU S Y,YANG Y X,et al.Exploring mechanism for vitrification damage of the cross-bred blastocysts of the yak via high-throughput sequencing[J].Acta Veterinaria et Zootechnica Sinica,2017,48(10):1871-1881.(in Chinese)
[7]	ZI X D,LIU S,XIA W,et al.Transcriptional profiles of crossbred embryos derived from yak oocytes in vitro fertilized with cattle sperm[J].Sci Rep,2018,8:11571.
[8]	YANG R F,XIONG X R,ZI X D.Effect of cysteine,insulin-like growth factor-1 and epidermis growth factor during in vitro oocyte maturation and in vitro culture of yak-cattle crossbred embryos[J].J Appl Anim Res,2019,47(1):463-466.
[9]	熊显荣,李键,字向东,等.犏牛高效生产新模式的建立[J].中国兽医学报,2019,39(5):1007-1013.XIONG X R,LI J,ZI X D,et al.Establishment of new model for cattle-yak efficient production[J].Chinese Journal of Veterinary Science,2019,39(5):1007-1013.(in Chinese)
[10]	BENVENISTE J,HENSON P M,COCHRANE C G.Leukocyte-dependent histamine release from rabbit platelets:the role of IgE,basophils,and a platelet-activating factor[J].J Exp Med,1972,136(6):1356-1377.
[11]	BATTYE K M,AMMIT A J,O'NEILL C,et al.Production of platelet-activating factor by the pre-implantation sheep embryo[J].J Reprod Fert,1991,93(2):507-514.
[12]	MINHAS B S,ZHU Y P,KIM H N,et al.Embryonic platelet activating factor production in the rabbit increases during the preimplantation phase[J].J Assist Reprod Genet,1993,10(5):366-370.
[13]	VANDENBERGHE L T M,HEINDRYCKX B,SMITS K,et al.Intracellular localisation of platelet-activating factor during mammalian embryo development in vitro:a comparison of cattle,mouse and human[J].Reprod Fert Dev,2019,31(4):658-670.
[14]	RYAN J P,O'NEILL C,AMMIT A J,et al.Metabolic and developmental responses of preimplantation embryos to platelet activating factor (PAF)[J].Reprod Fert Dev,1992,4(4):387-398.
[15]	FUKUDA A I,BREUEL K F. Implantation:effect of platelet activating factor on embryonic development and implantation in the mouse[J].Hum Reprod,1996,11(12):2746-2749.
[16]	O'NEILL C.Evidence for the requirement of autocrine growth factors for development of mouse preimplantation embryos in vitro[J].Biol Reprod,1997,56(1):229-237.
[17]	O'NEILL C.Autocrine mediators are required to act on the embryo by the 2-cell stage to promote normal development and survival of mouse preimplantation embryos in vitro[J].Biol Reprod,1998,58(5):1303-1309.
[18]	ROUDEBUSH W E,MASSEY J B,KORT H I,et al.Expression and presence of the platelet-activating factor receptor in human embryos[J].Fertil Steril,2003,79(5):1246-1248.
[19]	LEE S H,KIM D Y,NAM D H,et al.Role of messenger RNA expression of platelet activating factor and its receptor in porcine in vitro-fertilized and cloned embryo development[J].Biol Reprod,2004,71(3):919-925.
[20]	SAKELLARIOU M,DRAKAKIS P,ANTONOPOULOU S,et al.Intravenous infusion of PAF affects ovulation,fertilization and preimplantation embryonic development in NZB×NZW F1 hybrid mice[J].Prostaglandins Other Lipid Mediat,2008, 85(3-4):125-133.
[21]	BÓDIS J,PAPP S,VERMES I,et al."Platelet-associated regulatory system (PARS)" with particular reference to female reproduction[J].J Ovarian Res,2014,7:55.
[22]	OLTVAL Z N,MILLIMAN C L,KORSMEYER S J.Bcl-2 heterodimerizes in vivo with a conserved homolog,Bax,that accelerates programed cell death[J].Cell,1993,74(4):609-619.
[23]	YANG M Y,RAJAMAHENDRAN R.Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro[J].Anim Reprod Sci,2002,70(3-4):159-169.
[24]	REED J C.Proapoptotic multidomain Bcl-2/Bax-family proteins:mechanisms,physiological roles,and therapeutic opportunities[J]. Cell Death Differ,2006,13(8):1378-1386.
[25]	CHEN P,PAN Y,CUI Y,et al.Insulin-like growth factor I enhances the developmental competence of yak embryos by modulating aquaporin 3[J].Reprod Domest Anim,2017,52(5):825-835.
[26]	RICHANI D,GILCHRIST R B.The epidermal growth factor network:role in oocyte growth,maturation and developmental competence[J].Hum Reprod Update,2018,24(1):1-14.
[27]	CAVALERI F,SCHÖLER H R.Nanog:a new recruit to the embryonic stem cell orchestra[J].Cell,2003,113(5):551-552.
[28]	郭诗萌.猪早期胚胎发育中体外培养体系优化及OCT4、NANOG功能研究[D].哈尔滨:东北农业大学,2019.GUO S M.Optimization of in vitro culture system and function of OCT4 and NANOG on early embryonic development in pig[D].Harbin:Northeast Agricultural University,2019.(in Chinese)
[29]	CURRAN T,FRANZA B R JR.Fos and jun:the AP-1 connection.Cell,1988,55(3):395-397.
[30]	YU C H,ZHANG X Y,WANG L,et al.Interleukin-6 regulates expression of Fos and Jun genes to affect the development of mouse preimplantation embryos[J].J Obstet Gynaecol Res,2018,44(2):253-262.
[31]	HENSLEIGH H C,HUNTER A G.In vitro maturation of bovine cumulus enclosed primary oocytes and their subsequent in vitro fertilization and cleavage[J].J Dairy Sci,1985,68(6):1456-1462.
[32]	PFAFFL M W.A new mathematical model for relative quantification in real-time RT-PCR[J].Nucleic Acids Res,2001,29(9):e45.
[33]	VANDENBERGHE L T M,HEINDRYCKX B,SMITS K,et al.Platelet-activating factor acetylhydrolase 1B3(PAFAH1B3) is required for the formation of the meiotic spindle during in vitro oocyte maturation[J].Reprod Fert Dev,2018,30(12):1739-1750.
[34]	LI X M,SAGAWA N,IHARA Y,et al.The involvement of platelet-activating factor in thrombocytopenia and follicular rupture during gonadotropin-induced superovulation in immature rats[J].Endocrinology,1991,129(6):3132-3138.
[35]	KORDAN W,STRZEŻEK J.Effect of platelet-activating factor on motility parameters and plasmalemma integrity of boar spermatozoa[J].Anim Sci Pap Rep,2002,20(1):37-45.
[36]	RYAN J P,SPINKS N R,O'NEILL C,et al.Implantation potential and fetal viability of mouse embryos cultured in media supplemented with platelet-activating factor[J].J Reprod Fertil,1990,89(1):309-315.
[37]	VIERGUTZ T,LÖHRKE B.Role of the platelet-activating factor and its receptor in the proliferative regulation of bovine ovarian granulosa cells[J].Cell Prolif,2007,40(6):949-960.
[38]	何翃闳,潘阳阳,张慧珠,等.FSH对牦牛卵母细胞EGF、EGFR表达及其细胞凋亡的影响[J].畜牧兽医学报, 2018,49(9):1899-1907.HE H H,PAN Y Y,ZHANG H Z,et al.The effects of follicle-stimulating hormone (FSH) on the expression of EGF and EGFR in yak oocytes and apoptosis[J].Acta Veterinaria et Zootechnica Sinica,2018,49(9):1899-1907.(in Chinese)
[39]	赵天,李谷月,潘阳阳,等.Oct 4在牦牛卵母细胞及早期胚胎发育过程中的表达[J].畜牧兽医学报,2017,48(3):577-584.ZHAO T,LI G Y,PAN Y Y,et al.The expression of Octamer-binding transcription factor 4 gene (Oct 4) in yak (Bos grunneins) oocytes and preimplantation embryos produced by in vitro fertilization[J].Acta Veterinaria et Zootechnica Sinica, 2017, 48(3):577-584.(in Chinese)
[40]	KUES W A,SUDHEER S,HERRMANN D,et al.Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo[J].Proc Natl Acad Sci U S A,2008,105(50):19768-19773.

Effects of PAF Supplementation during in vitro Maturation Medium on Yak Oocyte Development Competence and Gene Expression

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