猪ADAR1基因cDNA全长克隆、序列信息及组织表达分析

doi:10.11843/j.issn.0366-6964.2019.06.003

Abstract

Abstract: This experiment was conducted to clone the full-length cDNA of porcine ADAR1 gene, and explore its expression in different tissues and backfat of pigs with different ages.The cDNA full-length sequence of ADAR1 gene was cloned using rapid-amplification of cDNA ends (RACE) and analyzed by bioinformatics. The real-time PCR was used to detect ADAR1 mRNA expression in different tissues and backfat of Large White pigs with different ages. A 6 259 bp cDNA sequence of porcine ADAR1 was cloned, which encoded 1 145 amino acids. Its amino acid sequence shared an identity equal or more than 85% with other mammals including human, chimpanzee, macaque, gibbon, cow, goat and sheep. The predicted ADAR1 had 2 Zα binding domains, 3 double-stranded RNA binding domains and a deaminase domain. The real-time PCR result showed that ADAR1 was expressed in heart, liver, spleen, lung, kidney, brain, muscle, small intestine and backfat. Furthermore, it was expressed in backfat of individuals with the ages of 7, 60, 120 and 180 days, showing an overall trend of going down firstly and going up then with the individual development. In this study, the full-length cDNA sequence of ADAR1 gene was successfully cloned in pigs, which was widely expressed in pigs and its expression levels were different in backfat of pigs with different ages. These findings provided a theoretical foundation for further function study of ADAR1.

CLC Number:

S828.2

ZHANG Yuebo, OUYANG Fengzheng, WANG Ligang, HOU Xinhua, LIU Xin, YAN Hua, ZHANG Longchao, WANG Lixian. The Full-length Cloning, Sequence Information and Expression Analysis of Porcine ADAR1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1135-1144.

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The Full-length Cloning, Sequence Information and Expression Analysis of Porcine ADAR1 Gene

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