F18大肠杆菌抗性型与敏感型苏太断奶仔猪十二指肠组织比较转录组分析

doi:10.11843/j.issn.0366-6964.2018.10.007

Abstract

Abstract:

To reveal the regulatory pathways and important candidate genes related to E. coli F18 resistance in Sutai pig (Duroc×Meishan pigs), and further explore the differences of hereditary basis in regulating E. coli F18 resistance between Chinese domestic and foreign pig breeds, Sutai and Meishan populations previously established with resistance and sensitivity to E. coli F18 were selected as experiment objects. Using transcriptome sequencing, we screened out the regulatory pathways and candidate genes related to E. coli F18 resistance in Sutai pig, then analyzed the expressions of candidate genes (proteins) in different serotype of E. coli F18 (F18ac and F18ab)-stimulated pig intestinal epithelial cell (IPEC-J2) by qPCR and Western blot. Additionally, the differential expressions of candidate genes in duodenal tissues between E. coli F18-resistant and E. coli F18-sensitive weaned piglets were detected by qPCR. The results showed that:1) there were 238 differentially expressed genes in duodenum between Sutai E. coli F18-resistant and E. coli F18-sensitive weaned piglets, which were mainly involved in toll-like receptor signaling pathway and glycosphingolipid biosynthesis-lacto and neolacto series pathway, including TLR5, IL-1β and FUT2 genes. 2) The mRNA expression levels of IL-1β, TLR5, FUT2 were all significantly up-regulated after F18ac-, F18ab-stimulated IPEC-J2, respectively (P<0.05), and the corresponding proteins expression were also obviously increased, which indicated that IL-1β, TLR5 and FUT2 might play important regulatory roles in the process of Sutai piglets responsing to E. coli F18 infection. 3) Tissue expressions analysis showed that the expression levels of TLR5, IL-1β, FUT2 genes in duodenum tissues between Sutai E. coli F18-resistant and E. coli F18-sensitive individuals were all significantly different (P<0.05). The expressions of TLR5, IL-1β genes in duodenum tissues between Meishan E. coli F18-resistant and E. coli F18-sensitive individuals showed extremely significant difference (P<0.01), but FUT2 expression was not significantly different (P>0.05). Combined with previous studies of molecular mechanism of resistance to E. coli F18 in Meishan and foreign pig breeds, this study revealed the differences of hereditary basis in regulating E. coli F18 resistance between Chinese domestic and foreign pig breeds. Toll-like receptor signaling pathway including CD14 and TLR5 probably played immune roles in regulating E. coli F18 resistance in Chinese domestic pig breeds such as Meishan pigs, while the glycosphingolipid biosynthesis-lacto and neolacto series pathway including FUT2 was associated with the formation of E. coli F18 receptor in foreign pigs.

CLC Number:

S828.2

WU Zheng-chang, FENG Hai-yue, HUANG Yan-jie, WU Li-si, WU Sheng-long, BAO Wen-bin. Comparative Transcriptome Analysis of Duodenal Tissues between Sutai E. coli F18-resistant and E. coli F18-sensitive Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2112-2123.

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Comparative Transcriptome Analysis of Duodenal Tissues between Sutai E. coli F18-resistant and E. coli F18-sensitive Weaned Piglets

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