PDF2和ODF1转录组测序筛选牛卵泡发育相关基因

doi:10.11843/j.issn.0366-6964.2018.02.009

Abstract

Abstract:

This study aimed to screen genes associated with bovine follicular development and investigate their expression patterns.Nine young cows were collected.The small follicle at onset of predeviation (PDF2) and the largest follicle at onset of deviation (ODF1) follicles were collected after estrus synchronization. The total RNA was extracted from separated granulosa cells for the transcriptome sequencing. The Gene Ontology analysis was carried out after searching the bovine Refseq database and screening differentially expressed genes. MAPK13,CYP19A1,GREB1,SERPINE2,GSTA5 genes were selected randomly for verification using qRT-PCR technology. Results suggested that 15 413 genes were obtained from PDF2 and ODF1 transcriptomes (RPKM value ≥ 0.5), of which 651 differentially expressed genes were selected (Fold change ≥ 2). GO assignments were used to classify the functions of the 651 genes, and which could be categorized into 3 main catrgories, biological process (41.66%), molecular function (17.24%), cellular component (41.10%). Combining with Genecards, 13 down-regulated and 17 up-regulated genes screened were associated with follicle development. qRT-PCR results indicated that the expression of CYP19A1, GREB1 and SERPINE2 in dominant follicles were very significantly greater than that in subordinate follicles (P<0.01). MAPK13 mRNA amounts in subordinate follicles were very significantly greater than that in dominant follicles (P<0.01). GSTA5 mRNA amounts in subordinate follicles were significantly greater than that in dominant follicles (P<0.05). qRT-PCR results were entirely consisted with transcriptome sequencing results, and further proved the reliability of the transcriptome sequencing, which provided a foundation for future investigation of the regulatory mechanisms involved in follicular development in cattle.

CLC Number:

S823.2

LI Peng-fei, MENG Jin-zhu, HAO Qing-ling, BI Xi-lin, WANG Kai, ZHU Zhi-wei,Lü Li-hua. Screening and Analysing of Genes Associated with Follicular Development in Bovine ODF1 and PDF2 Transcriptome[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 300-309.

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Screening and Analysing of Genes Associated with Follicular Development in Bovine ODF1 and PDF2 Transcriptome

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