RNA-Seq分析baeSR和acrB对鼠伤寒沙门菌相关毒力基因表达的影响

doi:10.11843/j.issn.0366-6964.2019.11.016

Abstract

Abstract: The aim of this study was to investigate the effects of baeSR and acrB gene deletions on the expression of virulence genes associated with Salmonella typhimurium. The differentially expressed genes of ciprofloxacin-resistant strain (CR), acrB gene-deficient strain (CRΔacrB), baeSR and acrB gene double-deletion strain (CRΔbaeSRΔacrB) were screened by using RNA-Seq technique. GO enrichment and KEGG pathway enrichment analysis were carried out on the selected genes to further explore the main functions of differentially expressed genes and the biological processes involved, and 7 genes with significant differences were selected for verification by real-time PCR. The results showed that, a total of 1 320 differentially expressed genes (absolute value of Fold change>2, Q-value<0.005) were screened between CRΔacrB and CR, of which 426 were up-regulated and 894 were down-regulated. There were 1 377 differentially expressed genes (absolute value of Fold change ≥ 2, Q-value<0.005) were screened between CRΔbaeSRΔacrB and CR, of which 405 were up-regulated and 972 were down-regulated. The virulence-related genes screened by the two comparison groups were mainly enriched in pathways such as two-component system, flagellar component, Salmonella infection, and bacterial invasion of epithelial cells. The results of real-time PCR are basically consistent with those of RNA-Seq. This study preliminarily analyzed and explored the role of baeSR and acrB genes in the virulence of Salmonella typhimurium, laying a foundation for further study on the pathogenic mechanism of Salmonella typhimurium.

CLC Number:

R378.22

LI Rui, GAO Haixia, XU Jun, WANG Rui, WANG Wenjing, ZHANG Ruiliang, ZHAO Xia, LI Lin. RNA-Seq Analysis of the Effects of baeSR and acrB on the Expression of Virulence Genes Associated with Salmonella typhimurium[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2318-2325.

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RNA-Seq Analysis of the Effects of baeSR and acrB on the Expression of Virulence Genes Associated with Salmonella typhimurium

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