一种新型无钩豆状囊尾蚴的鉴定和18S DNA序列分析

doi:10.11843/j.issn.0366-6964.2019.11.012

Abstract

Abstract: This study was designed to identify the difference between no-hook Cysticercus pisiformis (C. pisiformis) and hook C. pisiformis. Scolex's ultrastructures of C. pisiformis were observed by scanning electron microscope; PCR amplification and sequences analysis of 18S DNA of C. pisiformis were conducted and evolutionary tree comparison was performed among C. pisiformis and other animals' cysticercus in this study. The results showed that different ultrastructures of no-hook and hook C. pisiformis were mainly observed on rostellum, but the ultrastructures of suck and somite were alike. Most of the C. pisiformis taken out from the vesicles were in relative rest state. The rostellum of no-hook C. pisiformis looked like several oblate pastries stacked up together. Its surface was flatter and had a bigger nodule in the center, in which the diameter was about 20 μm. There were several layers of gear like patterns around the rostellum,forming an overlapping gear like structure. The front end of rostellum of hook C. pisiformis was blunt, and was connected to the chicken claw like hamula through the tegument muscular columns. The hamula were about 120-150 μm long, and its surface was smooth, rich luster and possessed hard appearance. Using 18S-P1 and 18S-P2 primers, the same target gene bands were obtained by PCR in the target genes of no-hook and hook C. pisiformis, culture medium of competent cells and bacterial plasmid. The sequencing results showed that the difference of the 750 bp sequences amplified by 18S-P1 primers was more significant than that of the 666 bp sequences amplified by 18S-P2 primers. Compared with evolution of Cysticercus of other animals, the no-hook C. pisiformis was related to C. saginate, while the hook C. pisiformis was related to C. solium. In conclusion, although both no-hook and hook C. pisiformis belong to the same genus, no-hook C. pisiformis is a new C. pisifomis being discovered.

CLC Number:

S852.734

PAN Yaoqian, KONG Lingyun, LI Peng, YUE Feng, WU Yuping, LIU Xingyou. Identification and 18S DNA Sequence Analysis of a New Type No-hook Cysticercus pisiformis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2283-2289.

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Identification and 18S DNA Sequence Analysis of a New Type No-hook Cysticercus pisiformis

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