牛囊胚ICM克隆多能性标记基因与表面标记的研究

doi:10.11843/j.issn.0366-6964.2015.07.009

Abstract

Abstract:

This study was designed to investigate the pluripotency-related markers of bovine ES cells so as to provide a basis for optimizing culture conditions of bovine ES cells and promoting its research progress.The inner cell mass (ICM) were separated from bovine IVF blastocysts，seeded on mouse embryonic fibroblasts cultured in 2i/LIF medium and then passaged mechanically.The pluripotent markers of the ICM-derived colonies were detected at passage 1 and passage 10 by immunostaining.Additionally，the ICM and trophectoderm (TE) isolated from bovine blastocysts by magnetic activated cell sorting were used for qRT-PCR to validate the differences in gene expression between ICM and TE.We have derived ICM colonies from bovine IVF blastocysts and cultured them to passage 10，which displayed typical stem cell morphology and expressed specific markers such as OCT4，SOX2，NANOG，SSEA1，SSEA4 and TRA-1-60.Moreover，the results of qRT-PCR showed that OCT4，SOX2 and NANOG were differentially expressed between ICM and TE，SOX2 showed the most significant difference.In conclusion，2i/LIF culture medium is helpful for the culture of the ICM-derived colonies from bovine IVF blastocysts，and SOX2 is more likely to be a candidate pluripotency-related marker gene of bovine ICM-derived colonies.

CLC Number:

S823.2

CUI Li-sha,ZHAO Xue-ming,HAO Hai-sheng,DU Wei-hua,MA You-ji，ZHU Hua-bin,WANG Zong-li. The Research of Pluripotency-related Marker Genes and Surface Markers in Bovine ICM Colonies[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1141-1149.

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References

［1］KIM E Y，NOH E J，PARK H Y，et al.Establishment of bovine embryonic stem cell lines using a minimized feeder cell drop［J］.Cell Reprogram，2012，14(6)：520-529.
［2］RATHJEN J，LAKE J A，BETTESS M D，et al.Formation of a primitive ectoderm like cell population，EPL cells，from ES cells in response to biologically derived factors［J］.J Cell Sci，1999，112(Pt5)：601-612.
［3］EVANS M J，KAUFMAN M H.Establishment in culture of pluripotential cells from mouse embryos［J］.Nature，1981，292（5819）：154-156.
［4］THOMSON J A，KALISHMAN J，GOLOS T G，et al.Isolation of a primate embryonic stem-cell line［J］.Proc Natl Acad Sci U S A，1995，92(17)：7844-7848.
［5］THOMSON J A，ITSKOVITZ-ELDOR J，SHAPIRO S S，et al.Embryonic stem cell lines derived from human blastocysts［J］.Science，1998，282(5391)：1145-1147.
［6］LI P，TONG C，MEHRIAN-SHAI R，et al.Germline competent embryonic stem cells derived from rat blastocysts［J］.Cell，2008，135(7)：1299-1310.
［7］STICE S L，STRELCHENKO N S，KEEFER C L，et al.Pluripotent bovine embryonic cell lines direct embryonic development following nuclear transfer［J］.Biol Reprod，1996，54(1)：100-110.
［8］CIBELLI J B，STICE S L，GOLUEKE P J，et al.Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells［J］.Nat Biotechnol，1998，16(7)：642-646.
［9］IWASAKI S，CAMPBELL K H，GALLI C，et al.Production of live calves derived from embryonic stem-like cells aggregated with tetraploid embryos［J］.Biol Reprod，2000，62(2)：470-475.
［10］MITALIPOVA M，BEYHAN Z，FIRST N L.Pluripotency of bovine embryonic cell line derived from precompacting embryos［J］.Cloning，2001，3(2)：59-67.
［11］SAITO S，SAWAI K，UGAI H，et al.Generation of cloned calves and transgenic chimeric embryos from bovine embryonic stem-like cells［J］.Biochem Biophys Res Commun，2003，309(1)：104-113.
［12］WANG L，DUAN E，SUNG L Y，et al.Generation and characterization of pluripotent stem cells from cloned bovine embryos［J］.Biol Reprod，2005，73(1)：149-155.
［13］CAO S，WANG F，CHEN Z，et al.Isolation and culture of primary bovine embryonic stem cell colonies by a novel method［J］.J Exp Zool A Ecol Genet Physiol，2009，311(5)：368-376.
［14］FURUSAWA T，OHKOSHI K，KIMURA K，et al.Characteristics of bovine inner cell mass-derived cell lines and their fate in chimeric conceptuses［J］.Biol Reprod，2013，89(2)：28.
［15］CONG S，CAO G，LIU D.Effects of different feeder layers on culture of bovine embryonic stem cell-like cells in vitro［J］.Cytotechnology，2014，66(6)：995-1005.
［16］NAGATOMO H，KAGAWA S，KISHI Y，et al.Transcriptional wiring for establishing cell lineage specification at the blastocyst stage in cattle［J］.Biol Reprod，2013，88(6)：158.
［17］MUÑOZ M，RODRÍGUEZ A，FRUTOS C，et al.Conventional pluripotency markers are unspecific for bovine embryonic-derived cell-lines［J］.Theriogenology，2008，69(9)：1159-1164.
［18］LA BLOMBERG，BPVL TELUGU.Twenty years of embryonic stem cell research in farm animals［J］.Reprod Dom Anim，2012，47(Suppl4)：80-85.
［19］OZAWA M，HANSEN P J.A novel method for purification of inner cell mass and trophectoderm cells from blastocysts using magnetic activated cell sorting［J］.Fertil Steril，2011，95(2)：799-802.
［20］OZAWA M，SAKATANI M，HANKOWSKI K E，et al.Importance of culture conditions during the morula-to-blastocyst period on capacity of inner cell-mass cells of bovine blastocysts for establishment of self-renewing pluripotent cells［J］.Theriogenology，2012，78(6)：1243-1251.
［21］VERMA V，HUANG B，KALLINGGAPPA P K，et al.Dual kinase inhibition promotes pluripotency in finite bovine embryonic cell lines［J］.Stem Cells Dev，2013，22(11)：1028-1042.
［22］BRACKETT B G，OLIPHANT G.Capacitation of rabbit spermatozoa in vitro［J］.Biol Reprod，1975，12（2）：260-274.
［23］ROSENKRANS C F，JR FIRST N L.Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro［J］.J Anim Sci，1994，72(2)：434-437.
［24］GONG G，ROACH M L，JIANG L，et al.Culture conditions and enzymatic passaging of bovine ESC-like cells［J］.Cell Reprogram，2010，12(2)：151-160.
［25］BUEHR M，MEEK S，BLAIR K，et al.Capture of authentic embryonic stem cells from rat blastocysts［J］.Cell，2008，135(7)：1287-1298.
［26］SCHMITTGEN T D，LIVAK K J.Analyzing real-time PCR data by the comparative C (T) method［J］. Nat Protoc，2008，3（6）：1101-1108.
［27］GOOI H C，FEIZI T，KAPADIA A，et al.Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains［J］.Nature，1981，292(5819)：156-158.
［28］ANDREWS P W，DAMJANOV I，SIMON D，et al.Pluripotency embryonal carcinoma clones derived from human teratocarcinoma cell line Tera-2：differentiation in vivo and in vitro［J］.Lab Invest，1984，50(2)：147-162.
［29］GOISSIS M D，CIBELLI J B.Functional characterization of SOX2 in bovine preimplantation embryos［J］.Biol Reprod，2014，90(2)：30.
［30］SUMER H，LIU J，MALAVER-ORTEGA L F，et al.NANOG is a key factor for induction of pluripotency in bovine adult fibroblasts［J］.J Anim Sci，2011，89(9)：2708-2716.
［31］YINDEE K J S，JIANG G，KANOK P，et al.Establishment and long-term maintenance of bovine embryonic stem cell lines using mouse and bovine mixed feeder cells and their survival after cryopreservation［J］.Science Asia，2000，26：81-86.
［32］VRANA K E，HIPP J D，GOSS A M，et al.Nonhuman primate parthenogenetic stem cells［J］.Proc Natl Acad Sci U S A，2003，100(suppl 1)：11911-11916.
［33］HARRIS D，HUANG B，OBACK B.Inhibition of MAP2K and GSK3 signaling promotes bovine blastocyst development and epiblast-associated expression of pluripotency factors［J］.Biol Reprod，2013，88(3)：74.
［34］BERG D K，SMITH C S，PEARTON D J，et al.Trophectoderm lineage determination in cattle［J］.Dev Cell，2011，20(2)：244-255.

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The Research of Pluripotency-related Marker Genes and Surface Markers in Bovine ICM Colonies

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