猪IRS4基因多态性、表达差异及其与脂肪沉积性状的关联分析

doi:10.11843/j.issn.0366-6964.2015.07.002

Abstract

Abstract:

This study aimed to detect polymorphisms of porcine IRS4 gene and its expression pattern in 17 tissues，and to identify gene markers associated with the QTL for fat deposition traits.Comparative sequence analysis of the IRS4 revealed 3 SNPs，including g.96C>G in the promoter region，a synonymous substitution g.1829T>C and a missense substitution g.1794T>C (p.Phe432Leu).Through RT-PCR assay，we found that IRS4 gene was abundantly expressed in hypothalamus and pituitary，but weakly expressed in other tissues.Furthermore，we genotyped the 3 SNPs in White Duroc×Erhualian F₂ intercross，Sutai pigs，Erhualian pigs and DLY［Duroc×(Landrace×Yorkshire)］three-way crossbred pigs and Chinese local wild boar by PCR-RFLP，then calculated allele frequencies and estimated the associations between those SNPs and backfat thicknesses (BFTs) at shoulder，chest，waist and hip，abdominal fat weight (AFW)，intramuscular fat content (IMF) and loin eye area (LEA) in these populations.The SNP g.96C>G was almost fixed in the F₂ intercross，so it was first excluded as a causative mutation (QTN) underlying this QTL.For both SNPs g.1794T>C and g.1829T>C，all western breeds (White Duroc and DLY) only had TT genotype，while，in the Erhualian population，the frequencies of CC genotypes at g.1794T>C and g.1829T>C were 65% and 100%，respectively.The results of association analysis showed that g.1794T>C was highly associated with all investigated traits in the F₂ intercross (P<0.01)，but associated only with IMF in Sutai pigs（P<0.05）and had no significant effect on Erhualian’s phenotypes；g.1829T>C was significantly associated with several fatness traits (e.g.BFT and LEA) in the F₂ intercross and Sutai pigs.Moreover，bioinformatics analysis demonstrated that the potential influence of the missense variant g.1794T>C on IRS4 protein function was limited.The results suggest that the SNP g.1794T>C is unlikely to be QTN，and the SNP g.1829T>C associated with fat deposition traits across populations could be used as marker for selection of these traits.

CLC Number:

S828.2

MA Huan-ban，LIU Xian-xian，YANG Jie，HUANG Wei-bing，DUAN Yan-yu，GUO Yuan-mei，MA Jun-wu. Porcine IRS4 Gene：Polymorphism，Differential Expression and Its Associations with Fat Deposition Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1084-1094.

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Porcine IRS4 Gene：Polymorphism，Differential Expression and Its Associations with Fat Deposition Traits

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