LPS诱导条件下猪小肠上皮细胞TLR4及其信号通路基因表达变化分析

doi:10.11843/j.issn.0366-6964.2015.07.003

Abstract

Abstract:

In this study，we exposed pig intestinal epithelial cells (IPEC-J2) to 0.1 and 1 μg•mL^-1 LPS for 2，4 and 6 h，respectively.Then，we estimated the relative mRNA expression of TLR4 and TLR4 signaling pathway-related genes (CD14，MyD88， TNF-α，IL-1β，IFN-α) using qPCR，which preliminary revealed the mechanism of the molecules related to inflammatory reactions in pig intestinal epithelial cells induced by enterotoxigenic Escherichia coli.Both concentrations of LPS upregulated the expression of TLR4 and its signaling pathway-related genes，and the expression level of all genes sharply increased from 4 to 6 h.The fold change of mRNA expression induced by 1.0 μg•mL^-1 LPS was significantly higher than that induced by 0.1 μg•mL^-1 LPS.The former stimulated the intestinal tract to produce stronger immune responses and more rapid development of inflammatory reactions.Above results suggested that LPS was released in the pig intestinal tract with E.coli infection，and upregulated the LPS receptor TLR4，leading to activation of the TLR4 signaling pathway.Given the dependency on myeloid differentiation factor 88 (MyD88) during signaling，stable upregulation of MyD88 and the cytokine cascade results in the release of large amounts of proinflammatory cytokines，causing inflammatory reactions，diarrhea and edema disease.

CLC Number:

S828.2

SUN Li，XIA Ri-wei，YIN Xue-mei，YU Li-huai，ZHU Guo-qiang，WU Sheng-long，BAO Wen-bin. Analysis of Differential Expression of TLR4 and TLR4 Signaling Pathway Genes under Lipopolysaccharide-induced Pig Intestinal Epithelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1095-1101.

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Analysis of Differential Expression of TLR4 and TLR4 Signaling Pathway Genes under Lipopolysaccharide-induced Pig Intestinal Epithelial Cells

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