牛源犬新孢子虫对BABL/c孕鼠胎盘激素和细胞因子调节功能的影响

doi:doi: 10.11843/j.issn.0366-6964.2016.08.021

Abstract

Abstract:

To invest the affection on regulating function of pregnant BALB/c mice placental hormones and cytokines caused by bovine Neospora caninum，in present study，we established the pregnant BALB/c mice model infected with bovine Neospora caninum，and pregnant BALB/c mice non-infected as negative control group.For collecting serum，mice of each group were sacrificed on the 12th，14th，16th，18th day after infection.The placental hormones CRH，CG，PRL and E3 in serum，and cytokines IFN-γ，IL-4 and TGF-β in placental cell suspension from pregnant mice were detected using ELISA method.Compared with control group，the results showed that CRH levels in pregnant BALB/c mice serum was significantly increased (P<0.05) on the 12th day and decreased significantly (P<0.01) on the 18th day after infection；while GC levels was decreased significantly (P<0.01) on the 12th day and increased significantly (P<0.05) on the 16th day；PRL levels was decreased significantly (P<0.05) on the 18th day；E3 levels was significantly increased (P<0.05) on the 12th day and decreased significantly (P<0.05) on the 16th day.At the same time，IFN-γ levels in pregnant BALB/c mice placental cell suspension was increased significantly (P<0.05) on the 18th day；while IL-4 and TGF-β levels were both increased significantly (P<0.05) on the 12th day.These results indicated that infection by bovine Neospora caninum in pregnant mice would lead to imbalance of placental hormones and cytokines regulative function and seriously affect placental blood flow，placenta mass transport，fetal growth and development.All these will lead to adverse pregnancy outcomes.

CLC Number:

S852.723

JIA Li-jun，MA Li，ZHANG Shou-fa，LI Nan-li，ZHAO Peng，LI Ji-xu. Placental Hormones and Cytokines Regulation in Pregnant BALB/c Mice Infected by Bovine Neospora caninum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1689-1695.

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Placental Hormones and Cytokines Regulation in Pregnant BALB/c Mice Infected by Bovine Neospora caninum

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