绵羊胃肠道游离氨基酸与小肽分布及其转运载体相关基因表达的研究

doi:10.11843/j.issn.0366-6964.2017.03.009

Abstract

Abstract:

This study aimed to clarify quantitative distributions of free amino acids (FAA) and small peptides (PAA) in gastrointestinal tract of sheep and the mRNA expression of CAT1 (cationic amino acid transporter), EAAT3 (acidic amino acid transporter), y⁺LAT2, ASCT2(neutral amino acid transporters), PepT1 (small peptide transporter), APN (aminopeptidase) and DPEP2 (dipeptidase) in gastrointestinal tract of sheep. A total of 6 healthy Small-tail Han sheep at the age of 18 months were slaughtered to collect chyme and tissues of rumen, duodenum, jejunum, ileum and cecum. Then concentrations of the most of FAA and PAA in supernatant of chyme were detected and the mRNA expression of related genes were quantified by RT-PCR. The results showed that: 1) The concentrations of the most of FAA from high to low was jejunum, duodenum, ileum, rumen, cecum. The concentrations of FAA in jejunum and duodenum were significant higher than that in other parts(P<0.05). Proportions of FAA in these parts were different. The concentrations of the most of PAA from high to low was duodenum, jejunum, ileum, cecum, rumen. Except for cecum and rumen, they were significant different from each other(P<0.05). Proportions of PAA in these parts were different. The proportion of PAA in total amino acids was the significantly lowest in jejunum(P<0.05). 2) CAT1 mRNA in jejunum had the highest abundance, but there were no significant difference among 5 parts. EAAT3, y⁺LAT2, PepT1 and APN mRNA had a similar expression pattern that they all had the highest abundance in ileum. ASCT2 mRNA had the highest abundance in duodenum, and DPEP2 mRNA had the highest abundance in cecum. Results indicate that concentrations and proportions of FAA in these parts are different, the same as PAA. The major secretion site of FAA is jejunum. The major absorption site of FAA is ileum. The major production site of PAA is duodenum. The major digestion site of FAA is jejunum. The major absorption site of FAA in mesenteric system is ileum. The absorbility of cationic amino acids in 5 parts are similar. Acidic amino acids and the most of neutral amino acids are absorbed major in ileum, but some of neutral amino acids are absorbed major in jejunum. This study provides the theory basis for amino acid nutrition in small intestine of sheep and for digestion and absorption regular pattern of protein of sheep.

CLC Number:

S826
S815

SUN Ya-jun, NI Zhi-he, CHEN Yu-lin, ZHANG En-ping, WANG Yong-jun. Study on Distribution of Free Amino Acid and Small Peptide and the Expression of Related Transporter Genes in Gastrointestinal Tract of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 462-473.

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References

［1］陈圆, 毛华明. 瘤胃微生物的小肽营养研究进展［J］. 饲料博览, 2005(5): 22-24.
CHEN Y, MAO H M. Research progress of small peptide nutrition in rumen microbials［J］.Feed Review, 2005(5): 22-24.(in Chinese)
［2］王梦芝, 曹恒春, 李国祥, 等. 反刍动物的理想氨基酸与小肠氨基酸供给模式的研究［J］. 饲料工业,2007,28(8):42-46.
WANG M Z, CAO H C, LI G X, et al. Ideal amino acid demand and supply pattern in small intestine of ruminant［J］. Feed Industry, 2007, 28(8):42-46.(in Chinese)
［3］徐凤霞. 绵羊瘤胃内寡肽代谢规律的研究［D］. 泰安：山东农业大学, 2004.
XU F X. A study on ruminal oligopeptides metabolism rulein sheep［D］. Tai'an: Shandong Agricultual University, 2004.(in Chinese)
［4］王丽娟. 蛋白饲料在鸡胃肠道中的肽类释放规律及其吸收特点研究［D］. 北京：中国农业大学, 2003.
WANG L J.Releasing law and absorption characteristics of peptides from protein feeds in gastrointestinal tract of chickens［D］. Beijing: China Agricultural University, 2003.(in Chinese)
［5］ERASMUS L J, BOTHA P M, MEISSNER H H. Effect of protein source on ruminal fermentation and passage of amino acids to the small intestine of lactating cows［J］. J Dairy Sci, 1994, 77(12):3655-3665.
［6］郭娟. 肠道微生态对大鼠小肠刷状缘酶活性影响的研究［D］. 武汉：华中农业大学, 2009.
GUO J. Effect of intestinal microecology on the activities of brush border enzymes activities in rat small intestine［D］. Wuhan: Huazhong Agricultural University, 2009.(in Chinese)
［7］许庆彪. 奶牛瓣胃上皮细胞对小肽的吸收机制及小肽转运载体1的特征研究［D］. 杭州：浙江大学, 2015.
XU Q B. The mechanism of small peptide absorption in bovine omasal epithelial cells and charactrization of bovine peptide transporter 1［D］．Hangzhou: Zhejiang University, 2005.(in Chinese)
［8］FERRARIS R P, DIAMOND J M. Crypt/villus site of substrate-dependent regulation of mouse intestinal glucose transporters［J］. Proc Natl Acad Sci U S A, 1993, 90(12):5868-5872.
［9］MCDOUGALL E I. Studies on ruminant saliva. 1. The composition and output of sheep’s saliva［J］. Biochem J, 1948, 43(1):99-109.
［10］解莹明. 奶牛消化道内小肽结合氨基酸的数量分布及其转运载体表达的研究［D］.杭州：浙江大学, 2014.
XIE Y M. Distribution of small peptide and its transporter gene expression in gatrointestine of dairy cows［D］．Hangzhou: Zhejiang University, 2014.(in Chinese)
［11］LING J R, ARMSTEAD I P. The in vitro uptake and metabolism of peptides and amino acids by five species of rumen bacteria［J］. J Appl Bacteriol, 1995, 78(2):116-124.
［12］SOTO R C, MUHAMMED S A, NEWBOLD C J, et al. Influence of peptides, amino acids and urea on microbial activity in the rumen of sheep receiving grass hay and on the growth of rumen bacteria in vitro［J］. Anim Feed Sci Technol, 1994, 49(1-2):151-161.
［13］徐晓锋, 金曙光, 欧宇, 等. 不同蛋白源日粮对绵羊瘤胃氨基酸和肽释放影响的研究［J］. 内蒙古农业大学学报:自然科学版, 2004, 25(2):9-12.
XU X F, JIN S G, OU Y, et al. The effects of different protein sources on concentration of amino acids and peptide in the rumen of sheep［J］.Journal of Inner Mongolia Agricultural University：Natural Science Edition, 2004, 25(2):9-12.(in Chinese)
［14］赵健亚. 不同SC/NSC日粮对湖羊胃肠道及血液营养生化指标的影响［D］. 扬州：扬州大学, 2005.
ZHAO J Y. Effect of different proportions ofdietary carbohydrate on gastrointestinal and blood nutritional or biochemical indices in Hu sheep［D］. Yangzhou: Yangzhou University, 2005.(in Chinese)
［15］JOHNSON L R,BARRET K E, GISHAN F K, et al. Physiology of the gastrointestinal tract ［M］. New York: Raven, 1994:1577-1622.(in Chinese)
［16］陈鼎. 小尾寒羊和滩羊肠道发育及其主要消化酶活性变化规律研究［D］. 杨凌：西北农林科技大学, 2008.
CHEN D. Regulation of intestine and its digestive enzymes activities between small-tailed Han sheep and tan sheep［D］.Yangling: North West Agriculture and Forestry University, 2008. (in Chinese)
［17］JOHNS J T, BERGEN W G. Studies on amino acid uptake by ovine small intestine［J］. J Nutr, 1973, 103(11):1581-1586.
［18］PHILLIPS W A, WEBB K E Jr, FONTENOT J P. Characteristics of threonine, valine and methionine absorption in the jejunum and ileum of sheep［J］. J Anim Sci, 1979, 48(4):926-933.
［19］吴信, 印遇龙, 李恒章. 精氨酸的代谢调控及其在猪营养中的研究进展［J］. 饲料与畜牧, 2011(9):8-12.
WU X, YIN Y L, LI H Z. Metabolic regulation of arginine and its research progress in swine nutrition［J］.New Feed, 2011(9):8-12.(in Chinese)
［20］张娜, 贾良梁. 甘氨酸在低蛋白肉鸡日粮中发挥关键作用［J］. 国外畜牧学:猪与禽, 2016，36(1):73-75.
ZHANG N, JIA L L. Glycine plays key role in low-protein broiler diets［J］.Animal Science Abroad:Pigs and Poultry, 2016,36(1):73-75.(in Chinese)
［21］MORALES A, BUENABAD L, CASTILLO G, et al. Low-protein amino acid-supplemented diets for growing pigs: effect on expression of amino acid transporters, serum concentration, performance, and carcass composition.［J］.J Anim Sci, 2015, 93(5):2154-2164.
［22］崔一喆, 王秋菊, 苏景, 等. 早期断奶对仔猪空肠和回肠兴奋性氨基酸载体1表达的影响［J］. 动物营养学报, 2016, 28(2):555-563.
CUI Y Z, WANG Q J, SU J, et al. Effects of early weaning on jenunal and ileal excitary amino acid aarrier 1 expression of piglets［J］. Chinese Journal of Animal Nutrition, 2016, 28(2):555-563.(in Chinese)
［23］FRIGERIO F, CASIMIR M, CAROBBIO S, et al. Tissue specificity of mitochondrial glutamate pathways and the control of metabolic homeostasis［J］. Biochim Biophys Acta, 2008, 1777(7-8):965-972.
［24］STOLL B, BURRIN D G, HENRY J, et al.Substrate oxidation by the portal drained viscera of fed piglets［J］. Am J Physiol, 1999, 277(1 Pt 1):E168-E175.
［25］骆巍, 杨钟, 孟丽云, 等. 5个不同家畜品种骨骼肌氨基酸含量分析［J］. 中国畜牧兽医, 2014, 41(8):117-122.
LUO W, YANG Z, MENG L Y, et al. Analysis of amino acids in skeletal muscles of 5 different livestock breeds［J］.China Animal Husbandry & Veterinary Medicine, 2014, 41(8):117-122.(in Chinese)
［26］MANN G E, YUDILEVICH D L,SOBREVIA L. Regulation of amino acid and glucose transporters in endothelial and smooth muscle cells［J］. Physiol Rev, 2003, 83(1):183-252.
［27］石常友, 宾石玉, 褚武英, 等. 藏猪肠道不同部位CAT1、EAAC1和PepT1 mRNA的特异性分布［J］. 广西师范大学学报:自然科学版, 2009, 27(3):71-75.
SHI C Y, BIN S Y, CHU W Y, et al. CAT1, EAAC1 and PepT1 mRNA in small intestine in different parts of in specific distribution in tibetan［J］. Journal of Guangxi Normal University:Natural Science Edition, 2009, 27(3):71-75.(in Chinese)
［28］谭会泽, 王修启, 苏海林, 等. 鸡不同肠段碱性氨基酸转运载体mRNA表达的差异性研究［J］. 畜牧兽医学报, 2007, 38(3):247-252.
TAN H Z, WANG X Q, SU H L, et al. Difference of cationic amino acid transporters mRNA expression in different intestinal segments of chicken［J］. Acta Veterinaria et Zootechnica Sinica, 2007, 38(3):247-252.(in Chinese)
［29］周英昊. 陕北白绒山羊碱性氨基酸转运载体基因cDNA克隆及时空表达分析［D］. 杨凌：西北农林科技大学, 2015.
ZHOU Y H. cDNA cloning and space-time expression analysis of cationic amino acid transporters gene in Shanbei white cashmere goat［D］. Yangling: North West Agriculture and Forestry University, 2015.(in Chinese)
［30］PAN M, MALANDRO M, STEVENS B R. Regulation of system y+ arginine transport capacity in differentiating human intestinal Caco-2 cells［J］. Am J Physiol, 1995, 268(4 Pt 1): G578-585.
［31］张爱华. 肉仔鸡肠道主要营养素转运载体mRNA表达发育规律的研究［D］. 北京：中国农业科学院, 2014.
ZHANG A H. Ontogenetic regulation of nutrient transporters in the small intestine of broilers［D］.Beijing: China Agricultural University, 2014.(in Chinese)
［32］ROMEO E, DAVE M H, BACIC D, et al. Luminal kidney and intestine SLC6 amino acid transporters of B0AT-cluster and their tissue distribution in Mus musculus［J］. Am J Physiol Renal Physiol, 2006, 290(2):F376-383.
［33］DEVÉS R, BOYD C A.Transporters for cationic amino acids in animal cells: discovery, structure, and function［J］. Physiol Rev, 1998, 78(2):487-545.
［34］KEKUDA R, TORRES-ZAMORANO V, FEI Y J, et al. Molecular and functional characterization of intestinal Na(+)-dependent neutral amino acid transporter B0［J］. Am J Physiol, 1997, 272(6 Pt 1):G1463-G1472.
［35］CHEN H, WONG E A, WEBB K E Jr. Tissue distribution of a peptide transporter mRNA in sheep, dairy cows, pigs, and chickens［J］. J Anim Sci, 1999, 77(5):1277-1283.

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Study on Distribution of Free Amino Acid and Small Peptide and the Expression of Related Transporter Genes in Gastrointestinal Tract of Sheep

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