基于cox1基因对中国青藏高原地区细粒棘球绦虫遗传多态性的研究

doi:10.11843/j.issn.0366-6964.2015.03.015

Abstract

Abstract:

The genetic variability of Echinococcus granulosus was investigated to uncover the genetic structure in the Tibet plateau，China.The complete mitochondrial cox1 genes about 1 609 bp from 47 isolates of E.granulosus collected from populations in the Tibet plateau were sequenced.Further sequences (retrieved from NCBI database) for the cox1 gene from the Tibet plateau and the Middle East were compared with the sequences analyzed in this study.The BLAST analysis of the sequences indicated 47 isolates belonged to G1 genetype (E.granulosus sensu stricto) and 10 haplotypes (C1-C10) were detected.The major haplotype C1 in the Tibet plateau showed a 100% homology with the reference haplotype in the Middle East and presented a worldwide distribution.The haplotype diversity (Hd) and nucleotide diversity (π) observed in the Tibet plateau were both lower than these in Middle East.The average negative values of Tajima's D and Fu’s Fs were negative in the two geographic populations and the populations were not genetically differentiated.These results suggest that E.granulosus in the Tibet plateau occurred demographic expansion or bottleneck effects after the introduction of founder haplotype in the Middle East.The population was developed under the geographical isolation.

CLC Number:

S852.734

WANG Ning，GU Xiao-bin，WANG Tao，YANG Guang-you. Genetic Variability of Echinococcus granulosus Determined by the Mitochondrial Cytochrome c Oxidase Subunit 1 Gene in the Tibet Plateau of China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(3): 453-460.

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Genetic Variability of Echinococcus granulosus Determined by the Mitochondrial Cytochrome c Oxidase Subunit 1 Gene in the Tibet Plateau of China

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