Myf5基因在马身猪和大白猪背最长肌中的发育性表达研究

doi:10.11843/j.issn.0366-6964.2014.12.024

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (12): 2081-2087.doi: 10.11843/j.issn.0366-6964.2014.12.024

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Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs

LI Wei¹，GAO Peng-fei¹，SHI Jian-zhong²，ZHANG Yan-qing¹，XU Kai¹，LIU Hong²，GUO Xiao-hong¹，LI Bu-gao¹，CAO Guo-qing^1*

(1.College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China；2.Datong Pig Breeding Farm，Datong 037000，China)

Received:2014-04-17 Online:2014-12-23 Published:2014-12-23

Abstract

Abstract:

The aim of this study was to investigate the expression patterns of Myf5 gene at the levels of mRNA and protein.The expression patterns of Myf5 gene at the levels of mRNA and protein in longissimus dorsi at 7 developmental stages (1-，30-，60-，90-,120-，150-，and 180-day-old) of Mashen and Large White pigs were studied by quantitative real-time PCR and Western-blot.The results showed that the highest mRNA relative expression of Myf5 gene in longissimus dorsi was at 1-day-old，then at 30-day old both in Mashen and Large White pigs.The mRNA relative expression of Myf5 gene decreased sharply at 60-day-old，then keep stable lower levels afterwards.The expression of Myf5 protein in longissimus dorsi of Mashen pig at 1-day-old was the highest and the lowest at 30-day-old.During the stages from 60- to 180-day-old，the relative expression of Myf5 peotein in Mashen pig was in waves with trend of increase and decrease.In Large White pig，the relative expressions of Myf5 protein at the stages before 60-day-old were significant greater than those at the stages after 60-day-old，wherease the expressions at detected stages before 60-day-old or after 60-day-old were not significant different.At the same stages，the relative expression of Myf5 protein in Mashen pig was always greater than that in Large White pig at all detectd stages apart from that at 60-day-old.The expression patterns of Myf5 at the levels of mRNA and protein were associated with the genetic background and the developmental stages of invididuals.

CLC Number:

LI Wei，GAO Peng-fei，SHI Jian-zhong，ZHANG Yan-qing，XU Kai，LIU Hong，GUO Xiao-hong，LI Bu-gao，CAO Guo-qing. Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2081-2087.

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Study on the Developmental Expression of Myf5 Gene in Longissimus Dorsi Tissue of Mashen and Large White Pigs

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