Abstract:

The purpose of this study was to investigate the effects of miniature pig combined anaesthetic (XFM) and xylazine on c-jun protein expression in different encephalic regions of rat. One hundred and fourteen SD rats were randomly divided into blank control group (B group，n=6)，saline solution control group (C group，n=36)，XFM group (H group，n=36) and xylazine group (X group，n=36) . Rats in B group were raised normally without any treatment，rats in C group were injected with normal saline，and rats in H group，X group were received XFM，Xylazine treatment，respectively. Each group was divided into six subgroups except B group: anesthesia induction stage (stage Ⅰ)，disappear stage of righting reflex (stage Ⅱ)，15 min after righting reflex disappear (stage Ⅲ)，35 min after righting reflex disappear (stage Ⅳ)，recovery stage of righting reflex (stage Ⅴ) and recovery of linear crawling (stage Ⅵ). Rats were decapitated in relative time，then cerebral cortex，hippocampus，cerebellum and brainstem tissue were separated. Proteins were extracted from these brain tissues，and c-jun protein content was detected by Western blot. Results showed that: (1) In C group，c-jun protein expressions had no significant changes in cerebral cortex，hippocampus，cerebellum and brainstem of rats（P>0.05） compared with B group；(2) In H group，c-jun protein expression increased in these encephalic regions，and decreased in stage Ⅵ (P<0.01) compared with B group；(3) In X group，c-jun protein expression increased in these encephalic regions(P<0.01)，and decreased in late stages (P<0.01) compared with B group. And c-jun protein expression level of X group was lower than that of H group (P<0.01). (4) c-jun protein expressions in these encephalic regions had no significant difference (P>0.05). During rats anesthetized with XFM and xylazine，c-jun protein expression level increased in cerebral cortex，hippocampus，cerebellum and brainstem tissue with the deepening of anaesthesia induction，and decreased when recovery from anesthesia. The results suggest that the expression changes of c-jun protein induced by XFM and xylazine in the encephalic regions may be one of the anesthesia mechanisms.