公牛MBL2基因多态性与精液品质及后裔生产性能的相关性研究

doi:10.11843/j.issn.0366-6964.2013.10.008

Abstract

Abstract:

The polymorphisms of MBL2 gene and their correlation to semen quality and progeny performance were investigated in order to find markers for semen quality and progeny performance in Chinese Holstein bulls. The polymorphisms of MBL2 gene were analyzed by the method of PCR-SSCP and sequencing in 218 adult Chinese Holstein bulls. The associations between mutation sites of MBL2 gene and semen quality and progeny performance were analyzed. The results showed that, there were five mutations, g.101 G/A, g.135 C/A, g.136 G/A and g.147 T/C in exon 1 and a novel SNP g.32 C/T in exon 2 (GenBank accession number: KC 832932). The g.101 G/A and g.135 C/A resulted in 31 Arg→31 Gln and 42 Pro→42 Gln, respectively. The site of g.101 G/A was significantly associated with sperm density (P＜0.05); the g.135 C/A was significantly associated with sperm motility and sperm density (P＜0.01); the g.136 G/A and g.147 T/C SNPs had very significant effect on sperm motility, sperm density, estimated breeding value of milk ( EBVM), estimated breeding value of fat (EBVF), estimated breeding value of protein ( EBVP) and estimated breeding value of somatic (EBVS) (P＜0.05); The g.32 C/T SNP had very significant effect on EBVS (P＜0.01). Five haplotype combinations were identified in exon 1 and the individuals with MN haplotype combination share the highest sperm motility, sperm density, EBVM, EBVF and EBVP to be positive for semen quality and dairy herd milk performance. The results indicate that the polymorphisms of MBL2 gene is correlated with semen quality and progeny performance in Chinese Holstein bulls.

CLC Number:

S823
S813.1

BAI Jing-yan, WU Jie, JIANG Xiao-qiang, WANG Gen-lin. Association of Polymorphisms of MBL2 Gene with Semen Quality and Progeny Performance of Bulls[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1561-1568.

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References

［1］张沅.奶牛遗传改良—牧场效应的重要基础［J］.中国乳业，2013，133：20-24.

［2］CORBET N J，BUMS B M，CORBET D H，et al. Genetic variation in growth, hormonal and seminal traits of young tropically adapted bulls ［J］. Proc Assoc Adv Anim Breed Genet，2009，18：121-124.

［3］LIU X Y，JU Z H，WANG L L，et al. Six novel single-nucleotide polymorphisms in SPAG 11 gene and their associations with sperm quality traits in Chinese Holstein bulls ［J］. Anim Reprod Sci，2011，129：14-21.

［4］YANG W C，TANG K Q，YU J N，et al. Effects of Mbo II and BspM I polymorphisms in the gonadotropin releasing hormone receptor (GnRHR) gene on sperm quality in Holstein bulls ［J］. Mol Biol Report，2011，38：3411-3415.

［5］SANG L，DU Q Z，YANG W C，et al. Polymorphisms in follicle stimulation hormone receptor, inhibin alpha, inhibin bata A, and prolactin genes, and their association with sperm quality in Chinese Holstein bulls ［J］. Anim Reprod Sci，2011，126：151-156.

［6］MARINA R S F，ANTONIO R，RACHEL J H，et al. Candidate genes associated with testicular development, sperm quality, and hormone levels of inhibin, luteinizing hormone, and insulin-like growth factor 1 in Brahman bulls ［J］. Biol Reprod，2012，87(3)：1-8.

［7］IP W K, TAKAHASHI K, EZEKOWITZ R A，et al. Mannose-binding lectin and innate immunity ［J］. Immunol Rev, 2009, 230：9-21.

［8］MOTOHIRO N，BRUCE Y M，MISATO O，et al. Subcellular localization and physiological significance of intracellular mannan-binding protein ［J］. J Biol Chem，2007，282(24)：17908-17920.

［9］WANG X J，JU Z H，HUANG J M，et al. The relationship between the variants of the bovine MBL2 gene and milk production traits, mastitis, serum MBL-C levels and complement activity ［J］. Vet Immunol Immunopathol，2012，148：311-319.

［10］ZHAO Z L，WANG C F，LI Q L，et al. Novel SNPs of the mannan-binding lectin 2 gene and their association with production traits in Chinese Holsteins ［J］. Genet Mol Res，2012，11(4)：3744-3754.

［11］VANDEGEIJN F E，ROOS A，DEMAN Y A，et al. Mannose-binding lectin levels during pregnancy: a longitudinal study ［J］. Hum Reprod，2007，22(2)：362-371.

［12］BODAMER O A，MITTERER G，MAURER W，et al. Evidence for an association between mannose-binding lectin 2 (MBL2) gene polymorphisms and pre-term birth ［J］. Genet Med，2006，8(8)：518-524.

［13］CHRISTIANSEN O B，NIELSEN H S，LUND M，et al. Mannose-binding lectin-2 genotypes and recurrent late pregnancy losses ［J］. Hum Reprod，2009，24(2)： 291-299.

［14］OGER P，LEDEE N，BULLA R，et al. Higher interleukin-18 and mannose-binding lectin are present in uterine lumen of patients with unexplained infertility ［J］. Reprod Biomed Onlin，2009，19(4)：591-598.

［15］SEYFARTH J，GARRED P，MADSEN H O. Extra-hepatic transcription of the human mannose-binding lectin gene (MBL2) and the MBL associated serine protease 1-3 genes ［J］. Mol Immunol，2006，43：962-971.

［16］WING J B，JACK D L，LEE M E，et al. Mannose-binding lectin is present in human semen and modulates cellular adhesion of Neisseria gonorrhoeae in vitro ［J］. Clin Exp Immunol，2009，157：408-414.

［17］赵成龙，杨茂成，夏祖灼. 种公牛育种值评定方法的比较［J］. 南京农业大学学报，1992，15(4)：68-74.

［18］SARFATY C K，JUNG M O, IN-WHA K, et al. A “Silent” polymorphism in the MDR1 gene changes substrate specificity ［J］. Science,2007, 315: 525-528.

［19］ANTON A K. SNPs, silent but not invisible ［J］. Science, 2007, 315: 466-467. 10.1126/science.1138239.

［20］SHABALINA S A, SPIRIDONOV N A, KASHINA A. Sounds of silence: synonymous nucleotides as a key to biological regulation and complexity key to biological regulation and complexity ［J］. Nucleic Acids Res, 2013, 41(4)：2073-2094.

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Association of Polymorphisms of MBL2 Gene with Semen Quality and Progeny Performance of Bulls

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