青藏高原细粒棘球绦虫的分子鉴定与遗传变异分析

doi:10.11843/j.issn.0366-6964.2013.09.015

Abstract

Abstract:

The aim of current study was to identify the genotype and genetic variation of Echinococcus granulosus in the Tibetan plateau of China, and provide the basic molecular data needed for studies of the molecular diagnosis, epidemiology, prevention and control of echinococcus diseases. The whole mitochondrial ND5 gene sequences of 43 isolates from the Tibetan plateau were analyzed. We found out that 42 isolates were belonged to E. granulosus G1 genotype, while one isolate from Tibetan sheep was identified as E. granulosus G6 genotype according to phylogenetic tree. There were 43 mutation sites in G1 isolates, which were separated in 27 haplotypes. The haplotype diversity and nuclear diversity were 0.940±0.028 and 0.001 93±0.001 14, respectively; And mean genetic distance between haplotypes was 0.002 6. Parsimony network showed a radialized expansion from a main founder haplotype H₉. The analysis of mismatch distribution showed a unimodal structure. Additionally, significant negative neutrality indices were detected. All these results suggested that the main genotype of E. granulosus in the Tibetan plateau was G1 with low genetic variability.

CLC Number:

S852.734

HU Dan-dan, WANG Ning, ZHONG Xiu-qin, WANG Jia-hai, YAN Ning, YANG Ai-guo, JIANG Zhong-rong, GUO Li, DENG Shi-jin, DAWA Tsering, KONG Wei-shu, LIU Tian-yu, ZHOU Xuan, XIE Yue,et al. Molecular Identification and Genetic Variation of Echinococcus granulosus in the Tibetan Plateau[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1438-1444.

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Molecular Identification and Genetic Variation of Echinococcus granulosus in the Tibetan Plateau

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