Abstract:

Factor XI deficiency caused by an insertion of 15 nucleotides in bovine Factor XI gene is a newly identified genetic defect that results in blood coagulation disorder in Japanese Black cattle. To analyze the distribution frequency of the defect gene in cattle population in China in current study, two new methods were developed(capillary electrophoresis-based genotyping and allele-specific amplification) to sensitively discriminate the alleles. Using these methods, 452 individuals were genotyped from 14 cattle breeds or populations, including Japanese Black bulls, a breeding herd and a commercial herd with Japanese Black cattle blood, four Chinese local breeds (Luxi, Yanbian, Menggu, Fuzhou), two composite breeds (Chinese Holstein, Xinjiang Brown), and six foreign breeds (Simmental, Charolais, Limousine, Angus, Montbeliard, Norwegian Red). The results showed that the defect allele frequency was 0.182, 0.389 and 0.242 in Japanese Black bulls, the breeding herd and commercial herd with Japanese Black cattle blood, respectively, 1 carrier was found in Yanbian cattle, and the mutation was not found in other cattle populations. The result suggest that the mutation only exist in cattle populations in Northeast Asia and Northeast region in China. The mutation will be detected and eliminated during crossing, improving and breeding the local cattle by Japanese Black cattle and then the unfavorable allele frequency will be reduced.