基于棉籽粕源发酵饲料的鸡血浆代谢组学研究

doi:10.11843/j.issn.0366-6964.2013.05.010

Abstract

Abstract:

The objective of this study was to investigate the effect of fermented feed from cottonseed meal mixed substrate on chickens plasma metabolites via metabolomic technology based on high performance liquid chromatography-mass spectromentry (LC-MS). The data of plasma metabolites profile collected by LC-MS, which included four groups of control group (CG), Candida tropicalis group (CT), Saccharomyces cerevisiae group (SC) and compound fermentation group (CF), were transformed and the peak characterization was obtained by the commercial software automatically. The total difference of metabolites among CG, CT, SC and CF were found through partial least square-discriminant analysis (PLS-DA), and the differential metabolites were selected according to the value of variable importance in the projection（VIP>1.5）and P (P<0.05). The results showed that significant difference of plasma metabolites were found after adding the fermented feed to diets and the relative contents of these metabolites were all increased significantly (P<0.01) compared with the control group. These metabolites were mainly phosphatidylcholine (PC), phophatidylethanolamine (PE), cholesterol ester (CE), sphingomyelin (SM), diacylglycerol (DG) and triacylglycerol (TG) in CT; PC, CE, SM, DG, TG in SC; and PC, PE, DG in CF. The influence of microorganism fermented feed from cottonseed meal mixed substrate on chicken plasma metabolites mainly was revealed in the significant increase of lipid metabolites. The PC and DG were common difference metabolites in all treatment groups.

CLC Number:

S831.4

NIE Cun-xi, ZHANG Wen-ju, YAN Li-dong, JIANG Li-xin, MA Gui-jun. A Metabolomics Study on Chicken Plasma Based on Fermented Feed from Cottonseed Meal Mixed Substrate[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 737-744.

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A Metabolomics Study on Chicken Plasma Based on Fermented Feed from Cottonseed Meal Mixed Substrate

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